Anti-anxiety Activity of Citrus paradisi var. duncan Extracts in Swiss Albino Mice


Anti-anxiety Activity of Citrus paradisi var. duncan Extracts in Swiss Albino Mice -A Preclinical Study 

Vikas Gupta1*, Parveen Bansal1, Junaid Niazi2, Kamlesh Kohli3, Pankaj Ghaiye4JHMR-CODE

1University Centre of Excellence in Research, BFUHS, Faridkot, India
2Department of Pharmaceutical Chemistry, Bahra Institute of Pharmacy, Patiala, India
3Department of Pharmacology, Guru Gobind Singh Medical College, BFUHS, Faridkot, India
4Department of Pharmaceutics, ACPTE, Sangrur, India

Review method: Single-blind; Peer reviewer comments: 1.


Background: Fragrances from aromatic oils of Citrus have been particularly attributed with mood enhancing properties by aroma therapists. Volatile oils are available in very small amounts hence authors intend to establish antianxiety activity of leaf extracts that can be made available for commercial purposes. Objectives: The present study was designed to authenticate the already established anti-anxiety activity (by using elevated plus maze model) of various extracts of the leaves of Citrus paradisi var. Duncan by authors using different animal models. Methods: Swiss Albino mice were treated with different doses of the leaf extracts (50, 100, 200 and 400 mg / kg p.o.) and Diazepam (2mg/kg, p.o) was used as a positive control. Antianxiety activity was determined using Y maze model, light dark model and hole board methods. Results: Results of study show that methanol extract in higher doses (100, 200, 400 mg/kg) possesses marked anti anxiety activity and was comparable to the effect produced by diazepam. Conclusion: The plant can be developed as a commercial source of anxiolytic agent. Further studies are in-process to isolate the active constituent responsible for this activity and mechanism of action.


Keywords: Anxiety, Grapefruit, Hole board test, Light dark model, Y maze

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