Research Article of International Journal of Animal Research
Molecular study for bovine herpes virus type 1 detection in Iranian cattle
Rassoul Hashemzehi1, Ayse Kilic2, Arman Akbarpour3, Esmaeil Mahmoudi3, Asghar Arshi4*
1. Department of Genetics, Fars Science & Research Branch, Islamic Azad University, Shiraz, Iran
2. Sivrice Vocational High School, University of Firat, 23119 Elazig-Turkey
3. Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
4. Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Bovine herpes virus type 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis, is a DNA virus. This pathogen is represents the most common viral pathogen found in cattle semen. The aim of the present study was to set up a of BHV-1 detection assay in bovine blood in Lorestan province using PCR assay. The blood samples of 285 cattle in Khoramabad, Azna, Aligoodarz, Borujerd and Poldokhtar were collected, total DNA was extracted and the region encoded the gI glycoprotein was amplified by PCR using specific primers. Out of 285 blood samples, 56 (19.64%) were positive for BHV-1 (468 bp). The highest and lowest frequencies of the bacterial infection were observed in Khoramabad and Borujerd cities with 21 and 12%, respectively. The results of this study demonstrated that PCR assay represent an excellent (suitable) alternative or additional tool for BHV-1 isolates detection. Finally the study revealed a high incidence of BHV-1 in the blood of Iranian cattle. Thus all cattle must be tested periodically for BHV-1 infection and antimicrobial drugs, to prevent BHV-1 occurrence in cattle must be used. The cattle must be free BHV-1 infection prior to use.
Keywords: Bovine herpes virus type 1, gI gene, PCR, Iran.