Privilege of Gum Arabic inclusion in semen extender compared with egg yolk on the Herri ram’s subsequent fertility outcomes

Privilege of Gum Arabic inclusion in semen extender compared with egg yolk on the Herri ram’s subsequent fertility outcomes

Mohamed Ali1 and Moustafa M. Zeitoun1,2*

1Department of Animal Production and Breeding, Qassim University, College of
2Department of Animal and Fish Production, College of Agriculture, Alexandria University, Egypt.

International Journal of Animal ResearchThis experiment was designed to scrutinize effects of inclusion of Gum Arabic versus egg yolk in either fresh or frozen extender for Herri ram’ semen on subsequent pregnancy rate. Semen ejaculates were collected from two rams. Two trials were attempted. Trial I, semen ejaculates were diluted with freshly prepared extenders either contained 10% EY (Control) or 6% GA (GA). Ewes were randomly selected and subdivided into 2 groups and inseminated. Trial II, extenders were frozen (-20°C) for 30 days before using, thawed and semen ejaculates from same rams were diluted and used in inseminations. Ewes were subdivided into 2 groups and inseminated with semen diluted in either control or GA6 extender. Results revealed that using fresh extender surpassed frozen one in achieving better pregnancy of the artificial insemination. Pregnancy rate derived of inseminations prepared by GA6 extender was significantly higher (P<0.0) than control extender. Therefore, using 6% of Gum Arabic in fresh extender can ultimately improve the fertility rate in Herri sheep.

Keywords: Gum Arabic inclusion, Semen extender, Egg yolk, Herri ram’s subsequent fertility outcomes

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How to cite this article:
Mohamed Ali and Moustafa M. Zeitoun. Gum Arabic inclusion, Semen extender, Egg yolk, Herri ram’s subsequent fertility outcomes. International Journal of Animal Research, 2017; 1:15. DOI: 10.28933/ijar-2017-10-2401


1 Akhter, S., Ansari, M.S., Andrabi, S.M., Ullah, N., Qayyum, M., 2008. Effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (Bubalus bubalis) bull semen, Reprod. Domest. Anim. 43; 272-278.
2 Ali, B.H., Al Moundhri, M.S., 2006. Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food Chem. Toxicol. 44, 1173-1183.
3 Ali, M., 2014. Relevance of type of breeding and single versus double sponge combined with PMSG on Herri ewes estrus exhibition and pregnancy rate. Global Vet. 13 (2): 166-172.
4 Ali, M., Musa M.M., Alfadul, S., Al-Sobayil, K., 2017. Consequences of adding Gum Arabic as a cryoprotectant on motility and viability of frozen stallion semen. Cryobiology, in press.
5 Amann, R.P., Hammerstedt, R.H., 1980. Validation of a system for computerized measurements of spermatozoa1 velocity and percentage of motile sperm. Biol Reprod., 2; 647-656.
6 Bousseau, S., Brillard, J.P., Guienne, M., Guerin, B., Camus, A., Lechat, M., 1998. Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin-based diluents. Theriogenology 50; 699-706.
7 Culham, A., Hill, G., 2009. Sheep Teaching & Research Center, Dept. of Animal Science, Michigan State University, 48824-1225.
8 Evans, G., Maxwell, C., 1987. Salmon’s artificial insemination of sheep and goats. Sidney: Butterworth pp. 66-76,108-109 & 158-161.
9 Gordon, I., 1983. Fixed time sheep artificial insemination. In: Controlled breeding in farm animals. Oxford, UK: Pergamon. pp. 197-208.
10 Hinson, J.A., Reid, A.B., McCullough, S.S., James, L.P., 2004. Acetaminophen-induced hepatotoxicity: role of metabolic activation, reactive oxygen/nitrogen species, and mitochondrial permeability transition. Drug Metab. Rev. 36, 805-822.
11 Hirai, M., Cerbito, W.A., Wijayagunawardane, M.P.B., Braun, J., Leidl., W., Ohosak, K., Matsuzawa, Q.T., Miyazawa, K., Sato., K., 1997. The effect of viscosity of semen diluents on motility of bull spermatozoa. Theriogenology, 47:1463-1478.
12 McNamee, B.F., O’Riordan, E.D., O’Sullivan, M., 2001. Effect of partial replacement of gum Arabic with carbohydrates on its microencapsulation properties. J. Agricul. Food Chem. 49, 3385-3388.
13 Moussa, M., Matinet, V., Trimeche, A., Tainturier, D., Anton, M., 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen, Theriogenology 57; 1695-1706.
14 Olesen, I., 1993. Effects of cervical insemination with frozen semen on fertility and litter size of Norwegian sheep. Livestock Prod. Sci. 37: 169-184.
15 Pervage , S., Hassan, M. R., Ershaduzzaman, M., Khandoker, M. A. M. Y., 2009. Preservation of liquid semen and artificial insemination in native sheep. J. Bangladesh Agril. Univ. 7(2): 305-308.
16 Petrie, A., Watson, P. 2002., Statistics for veterinary and animal science. Chapter 8 and 11. Blackwell science, 90-138.
17 Ye, A. Q., Edwards, P. J. B., Gilliland, J., Jameson, G. B., Singh, H., 2012. Temperature-dependent complexation between sodium caseinate and gum Arabic. Food Hydro. 26, 82-88.