Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin


Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin


Marwa A. Khalifa

Department of Animal and Poultry Physiology, Division of Animal and Poultry Production, Desert Research Center, Matariya, Cairo, Egypt.


International Journal of Animal ResearchThe aim of this investigation was to evaluate the efficiency of supplementing ram semen extender with melatonin on cryopreservation capacity of spermatozoa. A total of 80 ejaculates were collected from 5 Barki rams, 16 ejaculates each, by an artificial vagina throughout the period from January – February, 2017. Ejaculates of each collection session were pooled and diluted (1:10) with glycerolated Tris-citric egg yolk extender, and were split into 4 portions. The first portion served as control (melatonin-free), whereas the other 3 portions were supplemented with 0.1, 0.2 or 0.3 mM melatonin. The post-thaw objective assessment of cryopreserved spermatozoa, in all groups, was conducted by a computer-assisted sperm analysis (CASA) system. The results revealed that melatonin supplementation was positively correlated (P<0.01) with post-thaw total motility, progressive motility, viability, acrosome integrity, sperm cell membrane integrity, VSL, VAP and WOB (r= 0.88, 0.71, 0.78, 0.85, 0.96, 0.95, 0.68, 0.57 and 0.53, respectively. However, it was negatively correlated (P<0.01) with primary and secondary sperm abnormalities, as well as non-progressive motility and immotile spermatozoa (r= -0.73, -0.85, -0.49 and -0.76, respectively. These results elucidate that adding melatonin to ram semen extender substantially enhanced post-thaw sperm physical properties, which implies its powerful potential as an exogenous antioxidant supplement.


Keywords: Melatonin; ram; semen; cryopreservation; oxidative stress

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How to cite this article:
Marwa A. Khalifa. Improving cryopreservation capacity of ram spermatozoa by supplementing the diluent with melatonin . International Journal of Animal Research, 2017; 1:17. DOI:10.28933/ijar-2017-11-0202


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