Optimization of fermentation conditions for the production of 2,3-butanediol by Klebsiella pneumonia ZH-1 using response surface methodology


Optimization of fermentation conditions for the production of 2,3-butanediol by Klebsiella pneumonia ZH-1 using response surface methodology


LI Li, Feng HE, Jian-Guo XU and Qing-Ping HU *

College of Life Science, Shanxi Normal University, Linfen, Shanxi 041000, China


International Journal of Bioscience and Medicine

[Background] It has been studied that the yield of 2,3-butanediol (2,3-BD) producing strains is low and does not meet the requirements of industrial production of 2,3-BD. [Objective] It was important to improve the production of 2,3-BD by Klebsiella pneumonia ZH-1 in shaking flask. [Methods] The effects of temperature, pH and rotating speed on the production of 2,3-BD were studied by single factor test and response surface method. [Results] The optimal cultivation conditions stimulating the maximal production of 2,3-BD were as follow: initial pH, 7, temperature, 37 oC and rotating speed, 140 r/min. Under this optimized conditions, the predicted maximal 2,3-BD yield was 21.54 g/L, whereas the yield of 2,3-BD can reach to 22.04 g/L after the application of response surface methodology. [Conclusion] Response surface methodology was a promising method for optimization of 2,3-BD production.


Keywords: K. pneumonia ZH-1, response surface methodology, 2,3-butanediol

Free Full-text PDF