Review Article of International Journal of Hospital Pharmacy
Bioassay guided fractionation-an emerging technique influence the isolation, identification and characterization of lead phytomolecules
Neelesh Malviya*1, Sapna Malviya 2
* 1 Smriti College of Pharmaceutical Education, Indore
2 Modern Institute of Pharmaceutical Sciences (MIPS), Indore
Natural products have thus played an important role in drug discovery in the past and promise to provide still more drugs in the future. The search for a new drug from nature is based on a biological and ecological rationale. Natural products have provided many effective drugs. These include older drugs such as quinine and morphine and newer drugs such as paclitaxel, camptothecin, etoposide, mevastatin, and artemisinin. The discovery of novel drugs from nature is also important because many isolated molecules are quite complex, and would not be obtained by a simple synthetic approach. Most bioactive compounds of natural origin are secondary metabolites, i.e. species-specific chemical agents that can be grouped into various categories. A typical protocol to isolate a pure chemical agent from natural origin is bioassay-guided fractionation, meaning step-by-step separation of extracted components based on differences in their physicochemical properties, and assessing the biological activity, followed by next round of separation and assaying.
Keywords: Drug discovery, Natural products, bioassay-guided fractionation