Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark


Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark


Theophine Chinwuba Akunne1*, Bonaventure C. Obi1, Paul A. Akpa2, Sunday O. Udegbunam3, Adaobi J. Anaenugwu1

1Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
2Department of Pharmaceutics and Pharmaceutical Microbiology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria
3Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria


This study was aimed to evaluate antimicrobial and wound healing effects of root bark extract and fractions of Nauclea diderrichii Merrill (Rubiaceae). Dried root barks of N. diderrichii was extracted using methanol and dichloromethane (1:1) to obtain methanol-dichloromethane extract (MDE). The MDE was fractionated using chromatographic techniques to obtain n-hexane (HF), ethylacetate (EF) and methanol (MF) fractions. The antimicrobial effect was tested against selected organisms using agar diffusion and aInternational Journal of Traditional and Complementary Medicinegar dilution technique to determine the inhibition zone diameters (IZD) and minimum inhibitory concentrations (MICs). The excision wound model was used to assess the wound healing properties of MDE (5, 10 and 20%) and compared it with that of bacitracin and neomycin powder (BNP, standard) in rats. Phytochemical screening and acute toxicity test were performed using standard procedures. Results showed that extract and fractions exhibited antimicrobial effect with IZD (mm, 40 mg/ml) against tested organisms. The EF exhibited the best antimicrobial activity with IZD of 30, 8, 15, 8 and 12 against Staphylococcus aureus, Staphylococcus cohnii, Pseudomonas aeruginosa, Klebsiella aerogenes and Aspergillus flavus, respectively. The EF also gave an MIC (mg/ml) of 0.63, 20.00, 1.25, 20.00 and 0.63 for S. aureus, S. cohnii, P. aeruginosa, K. aerogenes, and A. flavus, respectively. The MDE showed a comparable wound healing effect with BNP, although with better effect on day 20 with 100% wound contraction while BNP gave 93.79%, post wound excision. Therefore, N. diderrichii root bark possesses antibacterial, antifungal and wound healing properties.


Keywords: Nauclea diderrichii, antibacterial and antifungal activity, phytochemical screening, wound healing.

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How to cite this article:
Theophine Chinwuba Akunne, Bonaventure C. Obi, Paul A. Akpa, Sunday O. Udegbunam, Adaobi J. Anaenugwu. Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark.International Journal of Traditional and Complementary Medicine 2017, 2:1. DOI:10.28933/ijtcm-2017-03-2101


References:

1. Chah KF, Eze CA, Emuelosi CE, Esimone CO. Antibacterial and wound healing properties of methanolic extracts of some Nigerian medicinal plants. J. Ethnopharmacol., 2006; 104:164-167.
2. Ramesh N, Viswanathan MB, Saraswathy A, Balakrishna K, Brindha P, Lakshmanaperumalsamy P. Phytochemical and antimicrobial studies of Begonia malabarica. J. Ethnopharmacol, 2002; 79: 129-132.
3. Fleischer TC, Ameade EPK, Sawer IK. Antimicrobial activity of the leaves and lowering tops of Acathospermum hispidum. Fitoterapia, 2003; 74: 130-132.
4. Patel JD, Shrivastava KM, Kumar V. Evaluation of some medicinal plants used in traditional wound healing preparations for antibacterial property against some pathogenic bacteria. Journal of Clinical Immunology and Immunopathology Research, 2009; 1(1):007-012.
5. Orwac C, Mutua A, Kindt R, Jamnadass R, Simons A. Agroforestree Database: a tree reference and selection guild version, 2009; 4.0. Available from http://www.worldagroforestry.org/af/treedbl
6. Lamidi M, Ollivier E, Gasquet M, Faure R, Nze-Ekekang L, Balansard G. Structural and antimalarial studies of saponins from Nauclea diderrichii bark. Adv Exp Med Biol. 1996;404:383.
7. Mustofa, Valentin A, Benoit-Vical F, Pélissier Y, Kone-Bamba D, Mallié M. Antiplasmodial activity of plant extracts used in West African traditional medicine. J Ethnopharmacol. 2000;73:145.
8. Di Giorgio C, Lamidi M, Delmas F, Balansard G, Ollivier E. Antileishmanial activity of quinovic acid glycosides and cadambine acid isolated from Nauclea diderrichii. Planta Med. 2006;72:1396.
9. Nwodo NJ, Agbo MO. Antitrypanosomal effects of methanolic extracts of Nuclea diderrichii (Merr.) and Spathodea campanulata stem bark. Journal of Pharmaceutical and Allied Sciences, 2010;7(5).
10. Trease GE, Evans WC, Test book of Pharmacognosy, 11th ed. Brailliare Tindall and Macmillian Publishers, London. 1989; Pp: 176- 180.
11. Harborne JB. Phytochemical Methods: a guide to modern techniques of plant analysis. 2nd edition. London, Chapman and Hall, 1988; pp:55–56.
12. Lorke D. A new approach to practical acute toxicity testing. Arch of toxicol, 1983; 53: 275- 287.
13. Akunne TC, Okafor SN, Okechukwu DC, Nwankwo SS, Emene JO, Okoro BN. Catfish (Clarias gariepinus) Slime Coat Possesses Antimicrobial and Wound Healing Activities. UK Journal of Pharmaceutical and Biosciences, 2016; 4(3):60-66.
14. Morton JJ, Malone MH. Evaluation of vulneary activity by an open wound procedure in rats. Arch. Int. Pharmacodyn, 1972; 196:117-126.
15. Okoli CO, Ezike AC, Akah PA, Udegbunam SO, Okoye TC, Mbanu TP, Ugwu E. Studies on wound healing and antiulcer activities of extract of aerial parts of Phyllanthus niruri L. (Euphorbiaceae). Am. J. Pharm. & Toxicol., 2009; 4(4):118-126.
16. Okoli CO, Akah PA, Okoli AS. Potentials of leaves of Aspillia Africana (composition) in wound care: an experiment evaluation. BMC Complement. Altern. Med., 2007; 7:24.
17. Nwonu P, Okoye TC, Akah PA, Ezike AC, Ejindu IA, & Eneh SE. Wound healing properties of stem bark extract of Tabebuia rosea. Journal of Pharmaceutical and allied sciences, 2010; 7(4):1115-1120.
18. Tohidi M, Khayami M, Nejati V. Meftahizade H. Evaluation of antimicrobial and wound healing of Pistacia atlantica and Pistacia khinjuk. Journal of Medical Plants Research, 2011; 5(17) pp. 4310-4314.
19. Velnar T, Bailey T, Smrkolj V. (2009). The wound healing process: an overview of the cellular and molecular mechanisms. The Journal of International Medical Research, 2009; 37 (5):1528-1542.
20. Özçelik B, Kartal M, Orhan I. (2011). Cytotoxicity, antiviral and antimicrobial activities of alkaloids, flavonoids, and phenolic acids. Pharmaceutical biology, 2011; 49(4), 396-402.
21. Okoye TC, Akah PA, Okoli CO, Ezike AC, Omeje EO, Odoh UE. Antimicrobial effects of a lipophilic fraction and kaurenoic acid isolated from the root bark extracts of Annona senegalensis. Evidence-Based Complementary and Alternative Medicine, 2012.
22. Barbakadze V, Mulkijanyan K, Gogilashvili L, Amiranashvil L, Merlani M, Novikova Z, Sulakvelidze M. Allantoin and pyrrolizidine alkaloids: free wound healing compositions from Symphytum asperum. Bull GA Nat. Acad. Sci., 2016; 3:159-164.
23. Panda P, Tripathy G. Wound healing activity of aqueous and methanolic bark extract of Vernonia arborea in Wistar rats. Nat Prod Radiance, 2009; 8:6–11.