Bio-enhancement effect of Bos primigenius indicus urine isolates on Curcumin anticancer activity using different human cell line models of A549, Hep-G2, MCF-7, Jurkat and K562


Bio-enhancement effect of Bos primigenius indicus urine isolates on Curcumin anticancer activity using different human cell line models of A549, Hep-G2, MCF-7, Jurkat and K562k


Hemanth Kumar Manikyam1, C.Ramesh2, Krishna Mohan Poluri3, Haranath Reddy KASIREDDY4, Charitha Devi Mekala5

1. Department of chemistry, North East Frontier Technical University, Arunachal Pradesh, India;
2. School of pharmaceutical Management, IIHMR University, Jaipur, and Rajasthan India;
3. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India;
4. Sri Venkateswara Gosamrakshana Shala, Tirumala Tirupati Devastanams, Andhra Pradesh, India;
5. Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.


International Journal of Traditional and Complementary MedicineThe study is conducted to explore possible utilization of cow urine and understanding complete biochemical compounds involved in possible therapeutic applications of cow urine therapy which was used from ancient times. In the present experiment inductively coupled plasma mass spectrometry (ICP-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) methods was used to analyze the complete mineral and compound detection. The test conducted using MTT method using human cancer A549, Hep-G2, MCF-7, Jurkat and K562 cell lines as part of the in vitro preclinical characterization of Cow urine extracted Curcumin and pure Curcumin compounds and compared against positive control Sodium lauryl Sulphate (SLS). More than 100% increment in cell killing at a concentration of 1.25 mg/ml recorded in the cell line for the cow urine extracted Curcumin and less than 70% for the pure Curcumin. Positive control SLS exhibited nearly 100% of killing cells.


Keywords: Cow urine, Curcumin, Anticancer, Ayurveda, cell lines, Bio-enhancement

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How to cite this article:
Hemanth Kumar Manikyam, C.Ramesh, Krishna Mohan Poluri, Harinath Reddy Kasireddy, Charitha Devi Mekala.Bio-enhancement effect of Bos primigenius indicus urine isolates on Curcumin anticancer activity using different human cell line models of A549, Hep-G2, MCF-7, Jurkat and K562 .International Journal of Traditional and Complementary Medicine 2017, 2:2. DOI: 10.28933/ijtcm-2017-11-2001


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