Microwave assisted Subcritical water extraction of Berberine hydrochloride from the roots of Berberis Aristata using Harmony search algorithm


Microwave assisted Subcritical water extraction of Berberine hydrochloride from the roots of Berberis Aristata using Harmony search algorithm

Hemanth Kumar Manikyam1 , C.Ramesh2 , Krishna Mohan Poluri3, Assif Assad4

1. Department of chemistry, North East Frontier Technical University , Arunachal Pradesh, India
2. School of pharmaceutical Management, IIHMR University, Jaipur, Rajasthan India.
3. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, India.
4. Department of Mathematics, Indian Institute of Technology Roorkee, Roorkee, India.


The aim of present study is to optimize the conditions of Berberine extraction from roots of Berberis Aristata by Microwave assisted subcritical water extraction (MASCW).MASCW method of extraction had shown reproducibility in respect to time, solvent usage, yield and extraction repeatability. Isoquinone Berberine, is one of the wide spread representatives belonging to family of protoberberine alkaloids abundantly present in roots and stem parts of Indian barberry. The subcritical factors like time, solvent/meal ratio, extraction repeatability, size of particles and temperature were investigated and were in accordance with experimental data and predicted data. Harmony search Algorithm (HSA) was used to study the effect of five subcritical parameters on the yield of Berberine .The extraction of berberine from roots of Berberis aristata was carried at 110-1700C using MASCW method using different variations of five subcritical parameters. The results show that all five factors (Time, Temperature, size of particles, repeatability of extraction and sample/liquid Ratio) have statistically significant effects on the berberine concentration. The results had suggested that experimental data concentration, 223.82 µg/ml and predicted data concentration, 214.854 µg/ml of berberine are in significant correlation at subcritical parameters like temperature 1700C, particle size of 0.65 mm, time of 70 mins, solvent/meal ratio of 12 and at maximum three repetitions. Thus the extracted berberine was precipitated as berberine hydrochloride by acid and further evaluated for NMR structural studies which confirms with standard berberine hydrochloride.

Keywords:  berberine hydrochloride, microwave assisted subcritical water extraction, Harmony search algorithm, phytochemistry, herbal remedies


Free Full-text PDF


How to cite this article:
Hemanth Kumar Manikyam, C.Ramesh, Krishna Mohan Poluri, Assif Assad. Microwave assisted Subcritical water extraction of Berberine hydrochloride from the roots of Berberis Aristata using Harmony search algorithm. Journal of Herbal Medicine Research, 2017, 2:19. DOI: 10.28933/jhmr-2017-11-0101


References:

1. Dostal J, Man S, Seckarova P, Hulova D, Necas M, Potacek M, et al. Berberine and coptisine free bases. Journal of Molecular Structure. 2004; 687(1–3):135–142. doi: 10.1016/j.molstruc.2003.09.018.
2. Birdsall TC, Kelly GS. Berberine: therapeutic potential of an alkaloid found in several medicinal plants. Alternative Medicine Review. 1997; 2(2):94–103.
3. Hayashi K, Minoda K, Nagaoka Y, Hayashi T, Uesato S. Antiviral activity of berberine and related compounds against human cytomegalovirus. Bio-org Medical Chemistry Letter. 2007; 17(6):1562–1564. Doi: 10.1016/j.bmcl.2006.12.085. [PubMed]
4. Guo JT, Li XY, Yang JH, Li L, Jian CG. Extraction of berberine hydrochloride by microwave-assisted pretreatment and Soxhlet extraction (in Chinese). Chemical Industrial. Engineering. Programme. 12: 89-92 (2003)
5. Geem, Z. W., Kim, J. H., & Loganathan, G. V. (2001). A new heuristic optimization algorithm: Harmony search. Simulation, 76(2), 60–68.
6. Liu B, Li WJ, Chang YL, Dong WH, Ni L. Extraction of berberine from rhizome of Coptidis chinenesis Franch. using supercritical fluid extraction. J. Pharm. Biomed. Anal. 41: 1056-1060 (2005)
7. Li Lei. Study on extraction process of berberine (in Chinese). Chem. Indus. Times 7: 47-52 (2008)
8. Miller LA, Carrier RL, Ahmed I. Practical considerations in development of solid dosage forms that contain cyclodextrin. Journal of Pharmacology Sciences. 2007; 96(7):1691–1707. doi: 10.1002/jps.20831. [PubMed]
9. Ong ES, Len SM. Pressurized hot water extraction of berberine, baicalein and glycyrrhizin in medicinal plants. Analytica Chimica Acta 482: 81-89 (2003)
10. R. Diao and Q. Shen, “Feature selection with harmony search,” Systems, Man, and Cybernetics, Part B: Cy-bernetics, IEEE Transactions on, vol. 42, no. 6, pp. 1509–1523, 2012.
11. Tripathi AN, Chauhan L, Thankachan PP, Barthwal R. Quantum chemical and nuclear magnetic resonance spectral studies on molecular properties and electronic structure of berberine and berberrubine. Magnetic resonance in chemistry. 2007; 45(8):647–655. doi: 10.1002/mrc.2019. [PubMed]
12. W. Geem, K. S. Lee, and Y. Park, “Application of harmony search to vehicle routing,” American Journal of Applied Sciences, vol. 2, no. 12, pp. 1552–1557, 2005.
13. Wang FM, Chen JH, Liu J, Lee FSC, Wang XR, Yang HH. Analysis of alkaloids in Coptis chinensis Franch. by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Analytica Chimica Acta 613: 184-195 (2008)
14. Xu PH, Gao Y, Zhang XQ, Zhang L, Xu K. Purification technology of total alkaloids from Rhizoma coptidis. Lishizhen Medical Materia Medica. Res. 18: 3079-3080 (2007)
15. Xiao GQ, Long LP, Wang JL, Li H. Comparison of microwave, ultrasonic wave and their combined extraction technology of the total alkaloid from Coptis Chinensis Franch. Chinese journal of spectroscopy laboratory. 3: 844-849 (2010)
16. Zuo F, Nakamura N, Akao T, Hattori M. Pharmacokinetics of berberine and its main metabolites in conventional and pseudo germ-free rats determined by liquid chromatography/ion trap mass spectrometry. Drug Metabolism and Disposition. 2006; 34(12):2064–2072. doi: 10.1124/dmd.106.011361. [PubMed]