Estimation of Some Phytochemicals in Swietenia macrophylla Leaves

Estimation of Some Phytochemicals in Swietenia macrophylla Leaves

*1Ushie O. A, 2Neji, P. A, 3Muktar, M, 2Ogah, E, 1Longbab, B.D and 1Olumide.V.B

1Department of Chemical Science, Federal University Wukari, Nigeria
2Department of Science Laboratory Technology, Binyaminu Usman Polytechnic, Hadejia. Jigawa State, Nigeria
3Dept of Chemical Sciences, Cross River University of Technology Calabar, Nigeria

Journal of Pharmaceutical Research and Reviews

Extract of the leaf for the detection of the phytochemicals were obtained by soaking 100g of the sample in 250ml ethanol for forty-eight hours with frequent agitation. The phytochemical screening of Swietenia macrophylla showed that tannin, phenol, flavonoid, terpenoids and alkaloids are present in the leaf extract. Quantitative determination of the detected secondary metabolites was carried out to know their percentages in the S. macrophylla leaves.The quantitative estimation of phytochemicals revealed that the various phytochemical constituents present in the leaf extract. In leaf extract of Swietenia macrophylla, the alkaloid content was 0.045 (9%), flavonoids content was 0.062 (12.4%), phenol content was 0.032 (6.4%) and tannin content was 0.043 (8.6%).

Keywords: Estimation, Phytochemicals, Swietenia macrophylla Leaves

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How to cite this article:
Ushie O. A, Neji, P. A, Muktar, M, Ogah, E, Longbab, B.D and Olumide.V.B. Estimation of Some Phytochemicals in Swietenia macrophylla Leaves. Journal of Pharmaceutical Research and Reviews, 2018; 2:15.


1 Aherne AS, Obrien NM (2002) Dietary flavonols: Chemistry, food content and metabolism. Nutrition 18: 75–81.
2 Akiyama H, Kazuyasu F, Yamasaki O, Oono T,Iwatsuki K (2001). Antibacterial action of several tannins against staphylococcus aureus. Journal of antimicrobial chemother, 48:487‐491.
3 Bajaj Y.S (1999). Medicinal and Aromatic Plants VSpringer. Biotechnology in Agriculture and Forestry. 24.
4 Bouayad N, Rharrabe K, Lamhamdi M, Nourouti NG, Sayah F (2011). Dietary effects of harmine, a β-carboline alkaloid, on development, energy reserves and a amylase activity of Plodia interpunctella Hübner [Lepidoptera: Pyralidae] Saudi Journal of Biological Sciences.;19(1):73–80.
5 Cheng, H.Y, Lin C.C, Lin T.C (2002) Antiherpes simplexvirus type 2 activity of casuarinin from thebark of Terminalia arjuna Linn. Antiviral Research, 55:447‐455.
6 Cowan, M.M., (1999): Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12: 564-582
7 Duthie, G.G,Duthie, S.J,, Kyle, J.A. M(2000)Plantpolyphenolsincancer and heart disease:Implications as nutritional antioxidants. Nutr Res Rev 13: 79–106
8 Edeoga, H. O., Okwu, D. E. & Mbaebie, B.O. (2005). Phytochemical constituents of some nigerian medicinal plants. .Afr. J Biotechnol.4:685–688.
9 Edeoga, H. O., Okwu, D. E. & Mbaebie, B.O. (2005). Phytochemical constituents of some nigerian medicinal plants. .Afr. J Biotechnol.4:685–688.
10 Hertog MGL, Kromhout D, Aravanis C, Blackburn H, Buzina R, Fidanza F, Giampaoli S, Jansen A, Menotti A, Nedeljkovic S, Pekkarinen M, Simic BS, Toshima H, Feskens EJM, Hollman PCH, Katan MB (1995) Flavonoids intake and long-term risk of coronary heart disease and cancer in seven countries study. Arch Intern Med 155: 381–386
11 Huang WY1, Cai YZ, Zhang Y.Natural phenolic compounds from medicinal herbs and dietary plants: potential use for cancer prevention.Nutr Cancer. 2010;62(1):1-20.
12 Iqbal, H., Ullah, R., Rooh, U., Khurram, M., Ulla, N., Baseer, A. et al., (2011). Phytochemical analysis of selected medicinal plants. Afr. J. Biotech. 10, 7487-7492.
13 Jenkins DJA, Diamandis, EP (2002) Flavonoids and steroid hormone-dependent cancers. J Chromatogr B, 777: 219– 232.
14 Karande, A. M., Kamble, H. V., Kumbhar, V. H., Kane, S. R., and C. S. Magdum Preliminary phytochemical screening of Glochidion ellipticum. European Journal of Experimental Biology, 2016, 6(4):41-45
15 Keli, S.O, Hertog M.G.L, Feskens, E.J.M, Kromhout, D (1996) Dietary flavonoids, antioxidant vitamins, and incidence of stroke. Arch Int Med 156: 637–642
16 Knekt P, Reunanen A, Jarvinen R, Maatela J (1996) Flavonoids intake and coronary mortality in Finland: A cohort study. Br Med J 312: 478–481.
17 Kolodziej H, Kiderlen AF (2005). Antileishmanialactivity and immune modulatory effects of tannins and related compounds onLeishmania parasitised RAW 264.7 cells.Phytochemistry 66:2056–2071.
18 Lin, L.U, Shu‐wen L, Shi‐bo J, Shu‐guang W (2004) Tannin inhibits HIV‐1 entry by targeting gp 41. Acta Pharmacol Sin, 25(2):213‐218.
19 Mann, J.1978. Secondary Metabolism. Oxford University press, London, pp. 154.
20 Mudasir, A.M, Rajesh. T.S., Rameashkannan M.V., Riyaz, A.P. & Muthu B. (2011). A
21 Comparative study of phytochemical analysis and antimicrobial properties of stigmas and stamens of Crocus Sativus L found in Kashmir. Adv Bio Tech. 11(6): 35-38
22 Narender, P.D, Ganga, R, Sambasiva, E, Mallikarjuna, T, Praneeth, V.S (2012). Quantification of phytochemical constituents and in vitro antioxidant activity ofMesua ferrea leaves. Asian Pac J Trop Biomed.;2(Suppl 2):S539–S542.
23 Patel, K, Gadewar, M, Tripathi, R, Prasad, S.K., and Dinesh Kumar Patel, A review on medicinal
24 importance, pharmacological activity and bioanalytical aspects of beta-carboline alkaloid ‘‘Harmine’’Asian Pac J Trop Biomed. 2012 Aug; 2(8): 660–664.
25 Sathya, V., Bharathidasan, R., Tamil, S.S., Solphia, R.N., Hakkiya, R & Prabakaran, M. (2013). Quantitative, phytochemical analysis and in vitro antibacterial activity of Bauchinia tomatosa, L. Journal of Natural Product Resources, 3(2),31-33
26 Sofowora, A. (1993) Medicinal Plants and Traditional Medcines Africa, Spectrum Books Ibadan
27 Stéphane Q, Tatiana V, Diana K, Michael J,Patrick P, Christian B et al. (2004). Main structural
28 and stereochemical aspects of theantiherpetic activity ofnonahydroxyterphenoyl‐containing Cglycosidicellagitannins. Chemistry &biodiversity, 1(2):247‐58
29 Subhashini, R., Mahadeva, U.S., Rao, P., Sumathi and Gayathri Gunalan (2010). A comparative phytochemical analysis of cocoa and green tea. Indian Journal of Science and Technology 3(2), 0974- 6846
30 Trease, G.E. and Evans, W.C (1989) Pharmacognsy 13th ed Baillere.Tindall London pp 176-180
31 Ushie O. A., Onen, A. I., Ugbogu, O. C., Neji, P. A. and Olumide.V. B (2016) Phytochemical Screening and Antimicrobial Activities of Leaf Extracts of Swietenia macrophylla ChemSearch Journal 7(2): 64 – 69
32 Ushie, O.A., Adamu, H.M.,Abayeh, O.J & Chindo, I.Y. (2013). Antimicrobial Activities of Chrysophyllum albidum Leaf extracts. International Journal of Chemical Sciences 6(1), 69-76 .
33 Yao, L. H.,Jiang, Y. M., Shi, J. Tom, F. A., Barber, A. Datta, S, N.,Singanusong, R. and &Chen, S. S. (2004) Flavonoids in Food and Their Health Benefits. Plant Foods for Human Nutrition 59: 113–122.