Review Article of Journal of Herbal Medicine Research
Pharmacological studies on the renal and hepatic effect of methanol leaf extract of Lupinus arboreus in rats
*1Ohadoma SC, 2Akah PA, 2Nworu CS, 2Okoye TC
1Department of Pharmacology, College of Medicine, Imo State University, PMB 2000, Owerri, Nigeria.
2Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, P.O. Box 3219, Nsukka.
Review method: Single-blind; Peer reviewer comments: 1.
Objective: To investigate the renal and hepatic effect of methanol leaf extract of Lupinus arboreus using experimental rats. Methods: Three groups comprising five rats each were used. Group II and III received 50 and 100 mg/kg of extract respectively. Group I served as negative control and received only normal saline (5 ml/kg). All administration was done once daily for 28 days. Urea and creatinine for renal effect were determined using Quimica Clinica applicado (QCA Test Kit, Spain); while hepatic marker enzymes were evaluated using Assay Kits (Randox Laboratories Ltd., United Kingdom BT 294 QY). Histopathological evaluation was carried out using light microscopy. Student’s t-test, ANOVA and Turkey-Kramer test were employed to assess significance of difference due to administration of extract and the control. Results: Treatment with extract did not produce significantly (P>0.05) changes in the hepatic marker enzymes when compared with the control. The mean creatinine levels showed non-significantly (P>0.05) differences when compared with the control. At 100 mg/kg, the extract exhibited significantly (p<0.05) elevation of blood urea levels, as well as urea: creatinine ratio, compared with the negative control. Histopathological assessment revealed that the liver architecture was preserved and no occurrence of structural changes in the kidneys. Conclusion: The leaf extracts of Lupinus arboreus are devoid of deleterious renal and hepatic effects.
Keywords: Lupinus arboreus, Chikadoma, leaf extract, renal and hepatic effects.