Insulin response of methanol fraction of Hensia crinita extract


Phytochemical profiling, oral glucose tolerance, body weight changes and insulin response of methanol fraction of Hensia crinita extract on Wistar Rats


Iwara A. Iwara*, Godwin O. Igile, Eyong Ubana Eyong, John M. Edward, Collins Igajah and Patrick .E. Ebong

Department of Biochemistry, Faculty of Basic Medical Sciences, University of Calabar, P.M.B 1115, Calabar, Nigeria.


Research Journal of Pharmacology and Pharmacy-2d CodePhytochemical profiling, oral glucose tolerance, body weight changes and insulin response fraction of Hensia crinita (H.C) leave extract was investigated in this study. Using  Gas Chromatography –mass spectrometry (GC-MS) analytical method for phytochemicals analysis, the methanol fraction of H.C showed the presence of fourteen compounds and revealing with respect to percentage abundance, high levels of Oleic acid (27.13%), Hexadecanoic acid (11.5%), ethylbenzene(9.92%), nonane (9.85%), 7-oxalic{4.1.0}heptane (9.83%), 1,2,3-trimethylbenzene (8.05%), Oxalic acid (7.42%)  as well as other compounds. Body weight showed significant increase (P<0.05) treated groups when compared to the control. Oral glucose tolerance level was also increased in the extract treated groups. A significant increase (P<0.05) in serum insulin level was observed in extract treated compared to diabetic control. Both ethanol crude extract and methanol fraction showed positive responses to oral glucose test, body weight changes and insulin levels activity and therefore may be incorporated in herbal decoctions in the management of diabetes.


Keywords: Phytochemical, antidiabetic, Oleic acid, Hensia crinita

Free Full-text PDF