Acute toxicity, anti-inflammatory and analgesic effects of Dilleniaceae


Acute toxicity, anti-inflammatory and analgesic effects of aqueous extract of Tetracera alnifoliaWilld. (Dilleniaceae)


Nsonde Ntandou GF a,b*, BoumbaL.S.b,d,  Gouollaly T.c,d,Makambila M.C.d, Ouamba J.M.c,  Abena A.A. a

 

  1. Laboratoire de Biochimie et Pharmacologie, Faculté des Sciences de la Santé, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo
  2. Laboratoire de Physiologie et Physiopathologie Animales, Faculté des Sciences et Techniques, Université Marien NGOUABI, Brazzaville, BP 69, Congo
  3. Unité de Chimie du Végétal et de la Vie, Faculté des Sciences, Université Marien NGOUABI, Brazzaville, B.P. 69, Congo
  4. Institut National de Recherche en sciences de la Santé, Cité Scientifique (ex-ORSTOM), Route de l’Auberge de Gascogne, Makélékélé, Brazzaville- Congo.

 


Research Journal of Pharmacology and Pharmacy-2d CodeThis work is part of the valorization of the traditional use of Tetracera alnifolia (Dilleniaceae). In this study we evaluated acute toxicity, anti-inflammatory and analgesic effects in rodent, and chemically screened its aqueous leaves extract. The phytochemical classic tests allowed to identify his various chemical families. Standard procedures by using OECD Guideline were followed to evaluate toxicity in mice. To assess anti-inflammatorythe carrageneen induced paw oedema and the pellet of cotton were used. Acetic acid and rat paw pressure methods served for the evaluation of the analgesic. This plant is very rich in flavonoids, cardiotonic heterosids and saponosides, and rich in alkaloids, steroids, terpenoides and tannins. Leaves aqueous extract is considered low-toxic with an LD50 greater than 2000 mg / kg. At this dose the extract exhibits signs of toxicity inclunding dyspnea and motor impairment. At the doses of 200 and 400 mg/kg the extract showed a significant effect on carrageenan-induced edema (p< 0.001), acetic acid induced abdominal cramps (p< 0.001) and on the nociceptive freshold(p< 0.001) and latency response of paw pressure (p< 0.001). These effects are due to presences of its secondary metabolites. This pharmacological results confirm the traditional uses of this plant underlined on stomach pain, swelling and gout.


Keywords: Traditional medicine, Tetracera alnifolia, Aqueous extract, Phytochemistry, Acute toxicity, Antiinflammatory, Analgesic.

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