Research Article of Research Journal of Pharmacology and Pharmacy
Antioxidant and sub-chronic toxicity studies on some biochemical parameters of the extract of Rhipsalis neves armondii K. Schuum. (Cactaeceae)
Theophine Chinwuba Akunne1*, Martha N. Ofokansi1, Maureen O. Akunne2, Bonaventure C. Obi1, Okereke E. Kingsley1, Emmanuel U. Onumajuru1, Precious Onunze1
1Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, 410001 Nsukka, Enugu State, Nigeria
2Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, University of Nigeria, 410001 Nsukka, Enugu State, Nigeria
Background: Rhisalis neves armondii K. Schuum. (Cactaceae) aerial parts preparation is used to treat inflammatory disorders and cancer in Nigerian ethnomedicine hence the need to study its antioxidant properties and to evaluate the toxicity profile.
Methods: The dried, ground, aerial parts of Rhipsalis neves armondii were extracted with a mixture of methanol and dichloromethane (1:1). The obtained extract (RNE) was employed for antioxidant and sub chronic toxicity studies. Antioxidant activity was determined using two in vitro models: ferricyanide reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, while ascorbic acid was used as standard antioxidant agent. Sub chronic toxicity tests were performed on some hematological and biochemical parameters such as total leukocyte count, differential white blood count and levels of the liver marker enzymes, in rats.
Results: The results showed that the RNE exhibited potent reducing power activity with mean absorbance value of 1.34 compared to 0.85 of ascorbic acid. The extract also produced a concentration-dependent percentage increase in DPPH radical scavenging activity at tested doses with IC40 values of 360 µg/ml and 130 µg/ml doses for RNE and ascorbic acid, respectively. There was no significant difference (p<0.05) in the liver marker enzymes (ALT, AST and ALT) and hematological parameters (neutrophils, lymphocyte, monocyte, eosinophil) of the animals at tested doses of the extract. The results also showed an LD50 of the extract greater than 5000 mg/kg body weight.
Conclusion: The result indicated that the extract of Rhipsalis neves armondii possesses antioxidant activity with good toxicological and safety profile.
Keywords: Rhipsalis neves armondii; antioxidant; sub-chronic toxicity; biochemical parameters.lytes.