Genetic Polymorphism of Tumor Necrosis Factor (Tnf – Α) and Their Association With Dengue Infection


Genetic Polymorphism of Tumor Necrosis Factor (Tnf – Α) and Their Association With Dengue Infection

SANTOS, A.C.M.; MOURA, E.L.; SANTOS, B.R.C.; FERREIRA, J.M.; FIGUEIREDO, E.V.M.S.

Federal University of Alagoas

Research Journal of Pharmacology and Pharmacy-2d Code


Some studies have associated the genetic constitution of the host with the clinical manifestations of patients infected with dengue. The objective of this study was to investigate the association of polymorphisms SNP -308A / G promoter of the TNF-α gene region with the clinical and laboratory manifestations of dengue in Alagoas Agreste population. The genotypes of the subjects were identified by PCR in real time. Laboratory data and symptoms were collected from medical records of patients. Statistical analysis was performed with SPSS 20.0 Software. The study wasapproved by the Ethics Committee of the Federal University of Alagoas (report number 405940). Of the 67 patients, 58.2 % were female (n = 39), but without statistical difference (p = 0.179), mean age was 31.5 (SD ± 13.2). With regard to clinical manifestations, 86.6 % (n = 58) had fever, 80.6 % (n = 54) were headache, 55.2 % (n = 37) developed myalgia The genotypic profile of the TNF-α patients It was 3 % (N = 2) patients A / A, 17.9 % A / G (n = 12) and 79.1 % G / G (n = 53). Genotypic compared the clinical characteristics were not significantly associated. Since in comparison to the laboratory results, a significant association of G / G genotype with hematocrit (p = 0.02) was observed, hemoglobin (p> 0.001) and erythrocytes (p> 0.001). There was no association between other genotypes or between leukocytes (p = 0.13) and platelets (p = 0.39) with the G / G genotype, while 61.2 % of patients in this profile have submitted leukocyte levels below 4,000 / mm³. Of the patients, 58.2 % were women, results similar to work nacionais, as well as the clinical manifestation of febre. A study in Puerto Rico found that 71 % of patients had cefaleia7 in line with this study. The G/G genotype was prevalent in our cases, as well as in Rio de Janeiro population. This genotype seems to have implications on the variation of hematocrit, red blood cells and hemoglobin in our population, the same genotype showed protection against disease progression in Venezuela when combined with other SNPs. Fever and headache are the most common symptoms in our patients. The G / G genotype may be associated with variations in the values of blood components in our population.

Keywords: Dengue; Polymoprhism; TNF; Health research


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REFERENCES

 

  1. 1. Acioli-Santos, B et al. MBL2 gene polymorphisms protect against development of thrombocytopenia associated with severe dengue phenotype. Human immunology, v. 69, n. 2, p. 122-128, 2008.
  2. Chuansumrit, A et al. Tumour necrosis factor gene polymorphism in dengue infection: association with risk of bleeding. Paediatrics and international child health, v. 33, n. 2, p. 97-101, 2013.
  3. Fernandez‐Mestre, MT. et al. TNF‐α‐308A allele, a possible severity risk factor of hemorrhagic manifestation in dengue fever patients. Tissue antigens, v. 64, n. 4, p. 469-472, 2004.
  4. Ferreira, RAX et al. Circulating cytokines and chemokines associated with plasma leakage and hepatic dysfunction in Brazilian children with dengue fever. Acta tropica, 2015.
  5. Oliveira, ECL et; al. Alterações hematológicas em pacientes com dengue. Rev. Soc Bras Med Trop. 2009; 42(6):682-685.
  6. Fujimoto, DE; Koifman, S. Clinical and laboratory characteristics of patients with dengue hemorrhagic fever manifestations and their transfusion profile. Rev Bras.
  7. Gregory, CJ. et; al. Clinical and Laboratory Features That Differentiate Dengue from Other Febrile Illnesses in an Endemic Area – Puerto Rico, 2007 – 2008. Am. J. Trop. Med. Hyg., 2010; 82 (5), pp. 922-929.
  8. Xavier-Carvalho, C et al. Single nucleotide polymorphisms in candidate genes and dengue severity in children: A case–control, functional and meta-analysis study. Infection, Genetics and Evolution, v. 20, p. 197-205, 2013. Hematol Hemoter. 2014;36(2):115-120.