Review Article of Journal of Herbal Medicine Research
Burns Inoculated with Staphylococcus aureus Healed by Leaf Sheath Extract of Pisang Ambon (Musa acuminata)
Samsuar1, Pratika Viogenta1, Laila Susanti1, Yuli Wahyu Trimulyani1, Sutriyani1, Mohammad Kanedi2*
1Department of Pharmacy, Faculty of Mathematics and Sciences, Tulang Bawang University, Bandar Lampung, Indonesia
2Department of Biology, Faculty of Mathematics and Sciences University of Lampung, Bandar Lampung, Indonesia
Review method: Single-blind; Peer reviewer comments: 1.
Using parts of banana to cure diseases is a common practice for people in homeland of genus Musa. However, scientific researches on biomedical effects of a specific banana cultivar still leaves many questions. This study is intended to find out the healing effect of leaf sheath extract of pisang Ambon or Gros Michel banana, a non-Cavendish cultivar of Musa acuminata, on burn wound intentionally innoculated with Staphylococcus aureus. Eighteen male rabbits were divided into six groups: P0, P1, P2, P3, P4, and P5 each consisting of three rabbits. P0 is rabbits that received no treatment as the negative control; P1, P2, P3 and P4 respectively are received banana extract of 6.25%, 12.5%, 55%, and 50%; P5 are given bioplacenton ointment as the positive control. To inflict burn wound, the withers area on the animal’s back were shaved and anticepted using 70% ethanol and 10% iodine. On the shaved skin, burns was made by placing a heated brass coin weighted 10 g and a diameter of 2 cm, at 200oC for 5 seconds. All treatments were given topically three times daily. The recovery of burns is measured every three days for 14 days and the healing criteria were based on wound diameter changes. The results showed the extracts of pisang Ambon at the concentration of 12.5% and 25% are able to totally heal the wound on the day 14. While extracts of 6.25 % and 50% has not provided a complete recovery on day 14 but still give a significant healing in comparison to negative control. Thus, leaft sheath extract of Gros Michel banana is effectively heal burns infected by S. aureus.
Keywords: Pisang Ambon, Gros Michel Banana, Musa acuminata, Staphylococus aureus, Burns, Wound Healing