Biogenic synthesis of Adhatoda vasica L. Nees mediated silver nanoparticles

Biogenic synthesis of Adhatoda vasica L. Nees  mediated silver nanoparticles and their antibacterial, anticancer activity on Hep-G2 cell lines

 International Journal of Traditional and Complementary MedicineArumugam Sengottaiyan 1,*†, Chinnappan Sudhakar 1†, Kandasamy Selvam 2*, Thangaswamy Selvankumar 1, Muthusamy Govarthanan 1,3, Balakrishnan Senthikumar 2 and Koildhasan Manoharan 4

1 PG & Research Department of Biotechnology, Mahendra Arts and Science College (Autonomous), Kalippatti, Namakkal 637501, Tamil Nadu, India. 2 Centre for Biotechnology, Muthayammal College of Arts and Science, Rasipuram, Namakkal 637 408, Tamil Nadu, India 3 Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570752, South Korea 4 Raja Duraisingam Government Arts & Science College, Sivagangai.

The first two authors equally contributed this work

The present investigation has been studied with the green synthesis of silver nanoparticles (AgNPs) using medicinally valued Adhatoda vasica -Nees and to evaluate the antibacterial and anticancer activity against HEP-G2 (Human epithelium cells of liver cancer) cell lines. The UV-Vis spectroscopy results show a strong resonance centered on the surface of silver nanoparticles at 420 nm. Fourier transform infrared (FT-IR) spectroscopy study demonstrates A. vasica aqueous extract acted as the reducing and stabilizing agent during the synthesis. The X-ray diffraction (XRD) analysis confirmed that the synthesized AgNPs are single crystalline face-centered cubic in structure, average crystal size 21 nm. Scanning electron microscope–energy dispersive spectroscopy (SEM-EDS) image confirmed synthesis of relatively uniform nanoparticles. The EDS analysis of the nanoparticles dispersion, using a range of 2-4 keV, confirmed the presence of elemental silver, without any contamination. The antibacterial activities were carried out against pathogenic bacteria. The maximum zone of inhibition was observed in the synthesized AgNPs (10µg/mL) against Staphylococcus sp. (16mm), Klebsiella sp. (14.5mm). The cytotoxicity activity as evidence by MTT assay with HEP-G2 cell lines. The synthesized AgNPs are ready for the application in the field of nanomedicine against pathogenic bacteria and very good anticancer drug.

Keywords: Adhatoda vasica, silver nanoparticles, antibacterial, cytotoxicity, HEP-G2 cell lines.

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