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Antimicrobial activity of crude leaf extracts against Enterococcus faecalis

Research Article of International Journal of Traditional and Complementary Medicine Antimicrobial activity of crude leaf extracts from medicinal plants against Enterococcus faecalis Rachuonyo HO1* Gatheri GW2 Nyamache AK1 1Department of Microbiology, Kenyatta University 2Department of Plant sciences, Kenyatta University Review method: Single-blind; Peer reviewer comments: 1. Enterococcus faecalis is a Gram negative, commensal enteric bacterial pathogen usually located in the intestinal tracts of either animals or human beings. The pathogenic members of enteric bacteria’s are usually associated to infections that are characterized by enteric fevers, abdominal pain, and diarrhoea and vomiting. This study aimed at determining the effects of the selected medicinal plants extracts against Clinical isolate of Enterococcus faecalis obtained from Kenyatta University health Centre. Kirby Bauer method was used to determine the antimicrobial activity of the plants extracts against Enterococcus faecalis. All the plant extracts showed antimicrobial activity against Enterococcus faecalis with Tagetes minuta producing the largest average zones of inhibition of 18.67±1.03mm when compared to the other plant extracts. Vernonia lasiopus was more active at low concentrations (MIC 3.9µg/ml; MBC 5.0µg/ml) while Aloe secundiflora was less active (MIC 7.0µg/ml; 9.7µg/ml). Ciprofloxacin (5µg/ml) was used as a positive control producing an average zone of inhibition of 21.67±2.66mm while Methanol (33.50±2.56mm) and 4% Dimethyl sulphoxide (0.00±0.00mm) were used as negative control. Qualitative phytochemical screening showed the presence of four phytochemicals namely; saponins, tannins, alkaloids and flavonoids. The study provides insight about the antimicrobial activity of the plant extracts and if they can be used in treatment of as an antimicrobial agent against infections caused by Enterococcus faecalis. Keywords: Qualitative phytochemical screening; Antimicrobial agents; Enterococcus faecalis; Ciprofloxacin; Clinical isolate ...

Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of Glori lily (Gloriosa superba L.)

Research Article of Journal of Herbal Medicine Research Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of Glori lily (Gloriosa superba L.) S. Deivasigamani Department of Agronomy, PGP College of Agricultural sciences, (Affiliated to Tamilnadu Agricultural University, Coimbatore-3)  Palani Nagar, Namakkal, Tamilmadu, India Review method: Single-blind; Peer reviewer comments: 1. Field experiment were conducted at farmer’s field, Devanur village, Ariyalur District of Tamil Nadu during 2008 to study the Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of glori lily. There were Five different treatments viz., Control  (T0), 100 per cent recommended  N (T1), 75 per cent recommended N (T2), 50 per cent recommended N (T3) and 25 per cent  recommended N (T4). The treatments were replicated four adopting RBD design. The growth and yield attributes of glori lily viz., plant height, leaf area index, number of primary branches plant-1, number of secondary branches plant-1, number of tertiary branches plant1,drymatter production (kg ha-1), days to fifty per cent flowering, number of pods plant-1, number of seeds pod-1 and hundred seed weight (g), were favorably  increased by the application of 100 per cent  recommended N (T1).  Seed and tuber yield (Kg ha-1) were greatly increased and the maximum seed and tuber yield were recorded. The uptake of NPK by glori lily was significantly increased by the above treatment combination. Application of 100 per cent recommended N (120 kg ha-1) (T1) gave the highest Gross income, Net income and return per rupee invested. Based on the above results, it can be concluded that inorganic use of 100 per cent recommended N (120 kg ha-1) is found to be an appropriate agro-technique for augmenting the productivity and profitability of glori lily without altering the soil fertility. It is a basic constituent in all proteins, enzymes, chlorophyll, certain parts ...

Diana’s Diary. A Case-Study of Hypnotherapy

Case Report of International Journal of Traditional and Complementary Medicine Diana’s Diary. A Case-Study of Hypnotherapy József P. Vas* H-3527. Miskolc, Soltész NK u. 1. 1/5, Hungary This case-study accounts on a 25 year’s old woman’s serious suffering and recovery due to successful hypnotherapy conducted by the author. Diana, the patient was a salesgirl in a little store. She was inflicted numerous stab-wounds with culpable homicide, and her life depended on few seconds only. After having had a chest surgery she was gradually becoming anxious while reliving her trauma again and again, and she became ill suffering from posttraumatic stress disorder (PTSD), and she had to take antidepressant and anxiolytic drugs. Her Jungian type of psychotherapy including hypnotic sessions with Guiding Spirit Method divided into two periods. In the course of the first period longing for a year she was relieving from anxious attacks and intrusive traumatic images as characterized by PTSD, and she has become balanced mentally. Although, she had been quite well after the first period of therapy, a new serious problem came to light since she aborted twice, and she was unable to be pregnant again. Then, the second period of therapy started for her request, and ended when she became pregnant again, and she had a newborn baby, later. This study is divided into two parts. In the first part the patient will be the leading character, whose subjective report serves as a basis of the case-study. In doing so an intimacy of the report is felt as it is told in Diana’s voice written in her diary. The therapist’s interpretation upon the therapeutic process can be read in the second part. The patient made reports based on her own experiences and gave her written consent for publication. The client’s name mentioned in this paper is fictional and ...

Physiological Benefits Of Yogic Practices: A Brief Review

Review Article of International Journal of Traditional and Complementary Medicine Physiological Benefits Of Yogic Practices: A Brief Review Madanmohan Trakroo1 , Ananda Balayogi Bhavanani 2 * 1 Professor and Head, Department of Physiology, MGMCRI and Director, CYTER, Sri Balaji Vidyapeeth, Pillayarkuppam, Pondicherry - 607 402. 2 Deputy Director, Centre for Yoga Therapy, Education & Research (CYTER), Sri Balaji Vidyapeeth, Pillayarkuppam, Pondicherry 607 402. Background: Yoga is becoming popular worldwide and many studies and systematic reviews are being published with scientific evidence of its health promoting abilities and therapeutic potential in various psychosomatic conditions. Aims and objective: This short review paper takes a bird's eye view of some health promoting benefits of yoga with regard to different systems as understood by modern medical physiology. Possible mechanisms for such physiological, biochemical and psychological effects are elucidated with references. Conclusion: Though most of the research studies and systematic reviews give evidence of in enhancing physiological functions of virtually every system, more exhaustive and rigorous studies are still advocated required to establish these positive benefits in conclusive terms. It is important to not forget the qualitative aspects of such benefits in our quest for quantitative findings when dealing with mind-body therapies and lifestyle modifications that have preventive, promotive, rehabilitative and curative potential as an adjunct therapy. Keywords:  health, physiology, yoga, stress, yoga therapy ...

Factors Affecting the Utilization of Antenatal Care among Pregnant Women in Moba Lga of Ekiti State, Nigeria

Research Article of International Journal of Traditional and Complementary Medicine Factors Affecting the Utilization of Antenatal Care among Pregnant Women in Moba Lga of Ekiti State, Nigeria Owoseni Joseph Sina Department of Sociology, Ekiti State University Ado Ekiti, Nigeria. This study investigated factors that influence Utilization of antenatal cares among pregnant women in Moba Local Government Area of Ekiti state. Descriptive research design was used for the study. The population for the study consisted of all pregnant women who are receiving antenatal cares in maternity centres and hospitals in Moba Local Government Area of Ekiti State. The Simple random sampling technique was used to select the sample. This study concluded that knowledge and attitude of pregnant women towards the utilization of an antenatal care services are encouraging and determine to a large extent their interest in using the services. It was recommended that government should endeavour to provide both human and material resources needed for effective utilization of antenatal care services and the services should be made completely free so that women from low socio-economic statuses would be able to assess it. Keywords:  Utilization, Antenatal Care, Pregnancy, Women, Ekiti ...

Insecticidal Efficacy of Plumeria Species Leaf Extract on Two Economically Important Insects Populations: Mosquito (Anopheles) and Bean Weevils (Callosobruchus Maculatus)

Research Article of Journal of Herbal Medicine Research Insecticidal Efficacy of Plumeria Species Leaf Extract on Two Economically Important Insects Populations: Mosquito (Anopheles) and Bean Weevils (Callosobruchus Maculatus) Aguoru, C. U.*,  Offia, K.L. and Olasan, J. O. Department of Biological Sciences, University of Agriculture, Makurdi. Nigeria. Review method: Single-blind; Peer reviewer comments: 1. Insecticidal efficacy of two Plumeria species (P. rubra and P. obtusa) foliar extracts was tested on mosquito and beans weevil populations. The extent of efficacy was analysed using linear regression model. The aim was to determine if the plant, as a safer biological control method, could possibly solve the problem of increasing populations of these insects implicated in malaria ailment and food shortages respectively. Completely randomized experimental designs were set up with 4 interaction effects. Each treatment had 5 levels of concentration applied on the test organism and replicated 4 times. Number of dead organisms was recorded at a constant time interval. Percentage mortality was computed at 48hrs. Correlation coefficient R and Coefficient of determination R2 were computed. Equations connecting the dependent variable (mortality) and independent variable (extract concentration) were formulated with corresponding t-values at 95% confidence interval.  Residual statistics was also computed. Explanatory power of the extract-mortality model was depicted by analysis of variance. Reliability Statistics of the overall result was tested using the Cronbach's Alpha. As a result, P. obtusa yielded mosquito mortality rate of 86.2% while P. rubra was 88.75%.  P. obtusa and P. rubra accounted for 90% and 95% weevil mortality respectively.  Correlation coefficients were very high (Plumeria–mosquito =0.970; Plumeria-weevil =0.968) yielding high coefficients of determination R2 (94% and 93.7% respectively). The two linear regression equations thus allows for accurate predictability of the mortality rate of the insects with 95% confidence limit. Mosquito mortality rate= -7.812 + 38.875 x (Extract concentration). Weevil mortality rate=-4.875 + 39.250 x (Extract concentration). The explanatory power ...