A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates
Research Article of American Journal of Microbiology and Immunology A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions: E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords: Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2 ...
Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2.
Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA)
Research Article of American Journal of Microbiology and Immunology Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA) Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) This descriptive study was done on 101 milk samples obtained from clinically mastitic dairy cows in Assiut Governorate, Egypt. Staphylococcus aureus (S. aureus) was the main causative agent of clinical mastitis (34.65%) followed by S. saprophyticus (10.89%), S. intermedius and S. epidermidis (8.91%, for each). The other causative agents (non Staph. Spp.) were identified. Sensitivity test of S. aureus isolates was performed against 11 antimicrobial agents, where found that 21 S. aureus strains (60%) were methicillin resistant S. aureus (MRSA). Ten MRSA strains were subjected for: I- slime-producing factor on Congo Red Agar (CRA) plates phenotypically, as 6 isolates (60%) were positive for slime production. II- PCR which was optimized targeting mecA, icaA and icaD genes, where 5 isolates (50%) were positive for mecA gene. Six isolates (60%) and 8 isolates (80%) were positive for icaA and icaD genes, respectively. Five strains (50%) were positive for both icaA and icaD genes. Also 3 strains (30%) were positive for all mecA, icaA and icaD genes. Conclusion, it was concluded that bovine staphylococcal mastitis was the most predominant issue where S. aureus was the main cause. Detection of mecA gene in S. aureus isolates indicating that several cases suffering from S. aureus mastitis have an MRSA problem. Genotypic determination of mecA gene proved the most reliable method for detection of methicillin-resistant S. aureus. The present work paid an attention to the 3 MRSA strains (30%) were positive to all tested genes rather than slime production as the worst isolated strains all over this study (multidrug resistant, slime producing as well as carrying mecA, icaA and icaD genes). In vitro Enrofloxacin, Gentamicin and Doxycycline the most effective drugs for Staph. spp. clinical mastitis and should be recommended for treatment of such cases of bovine mastitis. Keywords: Cows, clinical mastitis, methicillin-resistant Staphylococcus aureus, ...
Cows, clinical mastitis, methicillin-resistant Staphylococcus aureus, mecA, icaA, icaD, slime factor.
Research Article of International Journal of Traditional and Complementary Medicine Antimicrobial activity of crude leaf extracts from medicinal plants against Enterococcus faecalis Rachuonyo HO1* Gatheri GW2 Nyamache AK1 1Department of Microbiology, Kenyatta University 2Department of Plant sciences, Kenyatta University Review method: Single-blind; Peer reviewer comments: 1. Enterococcus faecalis is a Gram negative, commensal enteric bacterial pathogen usually located in the intestinal tracts of either animals or human beings. The pathogenic members of enteric bacteria’s are usually associated to infections that are characterized by enteric fevers, abdominal pain, and diarrhoea and vomiting. This study aimed at determining the effects of the selected medicinal plants extracts against Clinical isolate of Enterococcus faecalis obtained from Kenyatta University health Centre. Kirby Bauer method was used to determine the antimicrobial activity of the plants extracts against Enterococcus faecalis. All the plant extracts showed antimicrobial activity against Enterococcus faecalis with Tagetes minuta producing the largest average zones of inhibition of 18.67±1.03mm when compared to the other plant extracts. Vernonia lasiopus was more active at low concentrations (MIC 3.9µg/ml; MBC 5.0µg/ml) while Aloe secundiflora was less active (MIC 7.0µg/ml; 9.7µg/ml). Ciprofloxacin (5µg/ml) was used as a positive control producing an average zone of inhibition of 21.67±2.66mm while Methanol (33.50±2.56mm) and 4% Dimethyl sulphoxide (0.00±0.00mm) were used as negative control. Qualitative phytochemical screening showed the presence of four phytochemicals namely; saponins, tannins, alkaloids and flavonoids. The study provides insight about the antimicrobial activity of the plant extracts and if they can be used in treatment of as an antimicrobial agent against infections caused by Enterococcus faecalis. Keywords: Qualitative phytochemical screening; Antimicrobial agents; Enterococcus faecalis; Ciprofloxacin; Clinical isolate ...
Qualitative phytochemical screening; Antimicrobial agents; Enterococcus faecalis; Ciprofloxacin; Clinical isolate
Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of Glori lily (Gloriosa superba L.)
Research Article of Journal of Herbal Medicine Research Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of Glori lily (Gloriosa superba L.) S. Deivasigamani Department of Agronomy, PGP College of Agricultural sciences, (Affiliated to Tamilnadu Agricultural University, Coimbatore-3) Palani Nagar, Namakkal, Tamilmadu, India Review method: Single-blind; Peer reviewer comments: 1. Field experiment were conducted at farmer’s field, Devanur village, Ariyalur District of Tamil Nadu during 2008 to study the Different Levels of Nitrogenous Fertilizer Application for Growth, Flowering and Yield Attributes of glori lily. There were Five different treatments viz., Control (T0), 100 per cent recommended N (T1), 75 per cent recommended N (T2), 50 per cent recommended N (T3) and 25 per cent recommended N (T4). The treatments were replicated four adopting RBD design. The growth and yield attributes of glori lily viz., plant height, leaf area index, number of primary branches plant-1, number of secondary branches plant-1, number of tertiary branches plant1,drymatter production (kg ha-1), days to fifty per cent flowering, number of pods plant-1, number of seeds pod-1 and hundred seed weight (g), were favorably increased by the application of 100 per cent recommended N (T1). Seed and tuber yield (Kg ha-1) were greatly increased and the maximum seed and tuber yield were recorded. The uptake of NPK by glori lily was significantly increased by the above treatment combination. Application of 100 per cent recommended N (120 kg ha-1) (T1) gave the highest Gross income, Net income and return per rupee invested. Based on the above results, it can be concluded that inorganic use of 100 per cent recommended N (120 kg ha-1) is found to be an appropriate agro-technique for augmenting the productivity and profitability of glori lily without altering the soil fertility. It is a basic constituent in all proteins, enzymes, chlorophyll, certain parts ...
Nitrogenous Fertilizer, Growth, Flowering and Yield Attributes of Glori lily, Economics, Uptake and postharvest soil nutrient status.
Case Report of International Journal of Traditional and Complementary Medicine Diana’s Diary. A Case-Study of Hypnotherapy József P. Vas* H-3527. Miskolc, Soltész NK u. 1. 1/5, Hungary This case-study accounts on a 25 year’s old woman’s serious suffering and recovery due to successful hypnotherapy conducted by the author. Diana, the patient was a salesgirl in a little store. She was inflicted numerous stab-wounds with culpable homicide, and her life depended on few seconds only. After having had a chest surgery she was gradually becoming anxious while reliving her trauma again and again, and she became ill suffering from posttraumatic stress disorder (PTSD), and she had to take antidepressant and anxiolytic drugs. Her Jungian type of psychotherapy including hypnotic sessions with Guiding Spirit Method divided into two periods. In the course of the first period longing for a year she was relieving from anxious attacks and intrusive traumatic images as characterized by PTSD, and she has become balanced mentally. Although, she had been quite well after the first period of therapy, a new serious problem came to light since she aborted twice, and she was unable to be pregnant again. Then, the second period of therapy started for her request, and ended when she became pregnant again, and she had a newborn baby, later. This study is divided into two parts. In the first part the patient will be the leading character, whose subjective report serves as a basis of the case-study. In doing so an intimacy of the report is felt as it is told in Diana’s voice written in her diary. The therapist’s interpretation upon the therapeutic process can be read in the second part. The patient made reports based on her own experiences and gave her written consent for publication. The client’s name mentioned in this paper is fictional and ...
PTSD, the patient’s self-report, hypnotherapy, Guiding Spirit Method, work with symbols
Review Article of International Journal of Traditional and Complementary Medicine Physiological Benefits Of Yogic Practices: A Brief Review Madanmohan Trakroo1 , Ananda Balayogi Bhavanani 2 * 1 Professor and Head, Department of Physiology, MGMCRI and Director, CYTER, Sri Balaji Vidyapeeth, Pillayarkuppam, Pondicherry - 607 402. 2 Deputy Director, Centre for Yoga Therapy, Education & Research (CYTER), Sri Balaji Vidyapeeth, Pillayarkuppam, Pondicherry 607 402. Background: Yoga is becoming popular worldwide and many studies and systematic reviews are being published with scientific evidence of its health promoting abilities and therapeutic potential in various psychosomatic conditions. Aims and objective: This short review paper takes a bird's eye view of some health promoting benefits of yoga with regard to different systems as understood by modern medical physiology. Possible mechanisms for such physiological, biochemical and psychological effects are elucidated with references. Conclusion: Though most of the research studies and systematic reviews give evidence of in enhancing physiological functions of virtually every system, more exhaustive and rigorous studies are still advocated required to establish these positive benefits in conclusive terms. It is important to not forget the qualitative aspects of such benefits in our quest for quantitative findings when dealing with mind-body therapies and lifestyle modifications that have preventive, promotive, rehabilitative and curative potential as an adjunct therapy. Keywords: health, physiology, yoga, stress, yoga therapy ...
health, physiology, yoga, stress, yoga therapy