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Detection of SARS-CoV-2 by real time Reverse Transcriptase-Polymerase Chain Reaction assay in pleural effusion

Case Report of International Journal of Case Reports Detection of SARS-CoV-2 by real time Reverse Transcriptase-Polymerase Chain Reaction assay in pleural effusion Gatti E. MD1, Ulisciani S. MD2, Guglielmo M.  MD1*, Tinivella M. MD2, Farina C. MD2, Ascenzi C. MD2, Righi L. MD3, De Renzi G. MD4, Ciacco C. MD1 1Respiratory Physiopathology Department and COVID Department, San Luigi Gonzaga   Hospital, Orbassano, Torino, Italy. 2COVID Department, San Luigi Gonzaga Hospital, Orbassano, Torino, Italy 3Pathology Unit, Department of Oncology, University of Turin at San Luigi Gonzaga Hospital, Orbassano, Torino, Italy. 4Microbiology Department, San Luigi Gonzaga Hospital, Orbassano, Torino, Italy. SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2) is a novel coronavirus identified for the first time in Wuhan (China) in 2019, responsible of the current pandemic infection known as Coronavirus-19 disease (COVID-19). Wide range of clinical presentation of COVD -19 has been observed, from asymptomatic carriers to ARDS. The common signs and symptoms of SARS-CoV-2 infection include fever, fatigue, dry cough, and dyspnoea; the severity of the disease is due to the impairment of the respiratory function. The radiological findings include a large variety of lesions; bilateral interstitial pneumonia is the most concerning presentation of COVID-19. Pleural involvement has been described in a minority of cases: pleural thickening had been observed in 32% of cases whereas pleural effusion is uncommon being described in only 5%. Furthermore, pleural involvement has been significantly associated with a worse prognosis. Coronavirus 2 (SARS-CoV-2), beyond the nasopharyngeal swab, has been detected in other samples; up to now, data about RT-PCR specific results in the pleural fluid of patients suffering from coronavirus disease 2019 5 (COVID-19) are very limited. The current gold standard for diagnosis is nucleic acid detection by real time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) in nasopharyngeal swab. In this report, a case of a positive RT-PCR for Sars-Cov-2 in ...

BEYOND PSEUDO-ACCOMODATIVE CORNEA LASIK (PAC LASIK). A PERSONAL INSIGHT

Case Report of International Journal of Case Reports BEYOND PSEUDO-ACCOMODATIVE CORNEA LASIK (PAC LASIK). A PERSONAL INSIGHT Tjahjono D. Gondhowiardjo, MD, PhD1,2* 1Dept. of Ophthalmology, Faculty of Medicine, University of Indonesia - Cipto Mangunkusumo “Kirana” Hospital, Jakarta, Indonesia. 2JEC Hospitals and Clinics, Jakarta, Indonesia. Laser in-situ keratomieleusis (LASIK) is a common intervention for young, active, ametropic individuals to improve their visual acuity. pseudo-accommodative cornea (PAC), a variant of LASIK, to correct ametropia among presbyopic patients is proven in maintaining good distant vision; yet, the satisfactory spectacle free reading vision is limited to the ageing progression. However, successful treatments do not guarantee patient’s satisfaction. Assesment of the objective topographic indicators, visual acuity, higher order abrasion, and contrast sensitivity; revealed the clarification of a mild headache as a personal subjective experience after the treatment. The role of the persistent, dominant eye, the brain perception, seems to be critical factor to a patient’s satisfaction. To a certain degree, the interplay amongst the optical part and it’s supporting tissue, within and between the eyeballs, as well as its relationship to the neurosensory parts of the visual systems after Lasik surgery have not yet been assessed and reported elsewhere. Keywords: Eyeballs; Lasik surgery; Neuro sensory parts; supporting tissue; topographic indicators; visual acuity ...

The Oklo spontaneous nuclear reactor case: a fireball startup creating the South Atlantic

Review Article of International Journal of Natural Science and Reviews The Oklo spontaneous nuclear reactor case: a fireball startup creating the South Atlantic Florent Pirot Independent researcher There is a need to clarify continental formation processes and the Oklo natural nuclear reactor happens to be in the exact position where such a natural phenomena needs to be to explain continental separation. New research points of the author are shown to fit with findings of the French Commissariat à l'énergie atomique here. Keywords: Oklo spontaneous nuclear reactor case; fireball startup; South Atlantic ...

“Organic food” – makes pharmaceutical industries work against insects – a danger for society in general

Review Article of Research Journal of Pharmacology and Pharmacy "Organic food" - makes pharmaceutical industries work against insects - a danger for society in general Florent Pirot Independent researcher The author’s works have shown in various ways the absence of dangerosity of pesticides for human health, as all the studies claiming to find effects forget the side contamination with alpha-emitting nanoparticulates (from radon, phosphated fertilizers, car fumes, coal ashes for instance) that interacts with the samples and destroys the scientific validity of the results. This paper looks at the issue of organic food in a different way, because organic food also thrives on the sheer claim that it benefits to insects, and the author’s results here show that this is not the case too. Keywords: "Organic food"; pharmaceutical industries work ...

CHALLENGES IN PURSUING NURSING EDUCATION AT THE GRADUATE LEVEL: MOTIVATORS, BARRIERS, AND PERSISTENCE

Research Article of Global Journal of Nursing Challenges in Pursuing Nursing Education at the Graduate Level: Motivators, Barriers, and Persistence Collette Loftin PhD, RN*, Angela Phillips DNP, APRN, Marietta Branson DHSc, RN West Texas A&M University As the United States [U.S.] nursing shortage continues, the need for a highly educated work force grows. Although, the Carnegie Report [1] advocated for a Bachelor of Science in Nursing [BSN] degree as entry into practice with a required Master of Science in Nursing [MSN] earned within 10 years, the necessity to pursue a graduate degree in nursing has not been as widely encouraged as the baccalaureate degree. Master’s prepared nurses are essential across a variety of health care settings to serve in leadership, management, and advanced primary provider positions, as well as in academic settings as faculty members and researchers. In 2017, approximately 17% of the nursing workforce held a master’s degree. This was up from 13.8% in 2013 [2]. While the percentage of nurses earning a master’s degree has risen gradually, the need for additional highly educated nurses persists as rapid advancements in health care technology, including telehealth and informatics, occur. During 2016 and 2017, the nursing program at the authors’ institution tasked the recruitment and retention committee with increasing enrollment in the graduate nursing program. The committees stated goal was increasing enrollment in all role specializations (nurse practitioner, education, and management). The committee identified two areas of particular concern: the need for family nurse practitioners in the nearby rural communities and a shortage of nursing faculty in the area as these areas had become challenging. Although the nursing program had been successful at retaining a majority of its students, recruitment of new students was challenging and thus became the key focus. Keywords: Challenges; Nursing Education; Graduate Level; Motivators; Barriers; Persistence ...

EXPLAINING THE DISTINCTIVE PORTUGUESE-JEWISH GENETIC HERITAGE : AN INTERDISCIPLINARY DEMONSTRATION THAT THE GARDEN OF EDEN OF THE BIBLE (ATLANTIS) WAS THE AZORES, DESTROYED BY A FLOW OF COSMIC FIREBALLS

Review Article of American Journal of Genetics Explaining the distinctive Portuguese-Jewish genetic heritage : an interdisciplinary demonstration that the Garden of Eden of the Bible (Atlantis) was the Azores, destroyed by a flow of cosmic fireballs Florent Pirot Independent researcher Genetics demonstrate a distinctive Jewish-Portuguese heritage, especially in central Portugal, and the explanation can be given through an interdisciplinary analysis, involving geophysics, language, and economics. The text of the Bible (and the parallel existence of the Atlantis concept) can be put into meaning thanks to the strict application of natural justice concepts and of a basic hypothesis on a cattle herding economy that involved high degrees of inequality (caste-like), the victims finding justice in a cosmic fireball event that destroyed the nobility, triggered as well volcanic eruptions, and pushed them out, with a tsunami, as they had climbed on pumice stones. Because of the high natural radioactivity involved, from the fireballs and volcanic fallout, and of salt in the sea, memories were pretty much erased and the event reconstituted only simplistically. Keywords: distinctive Portuguese-Jewish genetic heritage; cosmic fireballs ...