Evaluation of ameliorative potential of isolated flavonol fractions from Thuja occidentalis in lung cancer cells and in Benzo(a)pyrene induced lung toxicity in mice
Research Article of International Journal of Traditional and Complementary Medicine Evaluation of ameliorative potential of isolated flavonol fractions from Thuja occidentalis in lung cancer cells and in Benzo(a)pyrene induced lung toxicity in mice Avinaba Mukherjee1, Sourav Sikdar1 and Anisur Rahman Khuda-Bukhsh1* 1Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani-741235, West Bengal, India. Review method: Single-blind; Peer reviewer comments: 2. Relative anticancer potentials of six flavonol fractions (F1-F6) isolated from Thuja occidentalis extract were first evaluated against lung cancer cells A549 in vitro, and fraction- F4 showing the maximum protective ability, was then tested in mice intoxicated with Benzo(a)pyrene (BaP), a known carcinogen with major effect on lung, to learn if this could also have ameliorative action against lung toxicity and tissue damage in mice in vivo. Chemical nature of F1-F6 fractions was confirmed with specific flavonol confirmatory test and mass spectral analysis; all fractions were tested for their possible anticancer effects against A549 cells. Results suggested that fraction 4 (F4) had the strongest anticancer effect. When treated to BaP intoxicated mice, F4 induced recovery of damaged lung tissue, presumably through inhibition of ROS generation, and enhanced production of major antioxidant molecules, that in turn blocked PI3K-activated expressions of Akt. Decrease in Bcl2/Bax ratio, over-expression of p53 gene and activation of caspase 3 were observed in tissues of F4 treated mice, further confirming apoptotic cell death as its major target. The F4 fraction of Thuja occidentalis extract showed remarkable apoptotic potential against lung cancer cells and ameliorative ability against BaP induced lung toxicity. Keywords: Flavonol; benzo[a]pyrene toxicity; lung toxicity; PI3K/Akt pathway; reactive oxygen species (ROS), Thuja occidentalis ...
Flavonol; benzo[a]pyrene toxicity; lung toxicity; PI3K/Akt pathway; reactive oxygen species (ROS), Thuja occidentalis.
Review Article of Journal of Herbal Medicine Research Bak Foong Pill and Bak Foong Capsule Siyao Ma* Shen Zhen Tian Yue Pharmaceutical Co. ,LTD., Shenzhen, Guangdong, China Review method: Single-blind; Peer reviewer comments: 1. ABSTRACT Bak Foong pill is a well known prescription to treat gynecological disorders in China. It comprises with more than 20 Chinese herbs including Wuji, Antler, Turtle Shell, Oysters, Sang Cuttlebone, Ginseng, Astragalus, Angelica, White Peony Root, Cyperus, Aspartame, Licorice, Rehmannia, Chuanxiong, silver Bupleurum, Salvia, Yam and Gorgon et al.. Based on the theory of Chinese Medicine, its indications include Qi and blood deficiency, frail, weak waist, irregular menstruation and uterine bleeding vaginal discharge. Previous studies mainly focused on reporting Bak Foong pill. In this review, we will introduce Bak Foong capsule, a new type of Bak Foong preparation, and compare the difference between Bak Foong Pill and Bak Foong capsule based on their prescription composition, indications and modern clinical application. In conclusion, Bak Foong capsule has the same function as Bak Foong pill although Bak Foong capsule has less Chinese herbs. More studies on Bak Foong capsule will help us treat diseases like uterine bleeding vaginal discharge and know the possible difference between Bak Foong pill and Bak Foong capsule. Keywords: Bak Foong Pill, Bak Foong capsule, Wujibaifeng pill, Wujibaifeng capsule ...
Bak Foong Pill, Bak Foong capsule, Wujibaifeng pill, Wujibaifeng capsule
Review Article of Journal of Herbal Medicine Research The effects of Chinese Herbal Medicine on Blood- Brain Barrier Lihong Deng and Shao Liu* Zhongshan Asia Pacific Hospital, 3 Nanjiang Road, Zhongshan, Guangdong, China Review method: Single-blind; Peer reviewer comments: 1. ABSTRACT The blood-brain barrier (BBB), which can open under both physiology and pathophysiology, is an important diffusion barrier to brain. It can block many componds from blood and protect the brain. BBB is mainly built by cerebral endothelium, tight junctions, adherens junctions, astrocytes, and pericytes and extracellular matrix. Each element plays specific roles to protect the brain. Currently, one of the limitations to treat CNS diseases is that most drugs can not pass through the BBB and can not directly affect the focus of infection. So drugs which could help BBB open would help us to treat CNS diseases. Chinese herbal medicine has been wildly used in China to treat all kinds of diseases, and some of them, like Borneol, Acorus tatarinowii Schott, Moschus, Storax and Benzoin, has notably dual effects on the permeability of the BBB. They can both reduce and increase the the permeability of the BBB. More studies on the effects on Chinese herbal medicine’s effects on BBB may help us out of the limitation that brain drugs can not pass through the BBB. Keywords: blood-brain barrier, Chinese herbal medicine, Borneol, Acorus tatarinowii Schott, Moschus, Storax, Benzoin ...
blood-brain barrier, Chinese herbal medicine, Borneol, Acorus tatarinowii Schott, Moschus, Storax, Benzoin
Review Article of Journal of Herbal Medicine Research Chinese Herbal Medicine and Stroke- a Short Review Min Xu Hainan Radio & TV University, Longhua 2nd Cross Rd, Longhua, Haikou, Hainan, China Review method: Single-blind; Peer reviewer comments: 1. ABSTRACT Stroke is one of the primary cause of long- term disability. Although mounting studies focused on stroke research, currently, only available treatment for stroke is t-PA, with the limitation of a very narrow therapeutic window because of the risk of hemorrhage. So, new therapeutic methods for stoke are expected. Chinese herbal medicine has been used to treat stroke with a history of many centuries and is stilling wildly used in China. This review focuses on reporting the research progress of the Chinese herbal medicine including Acorus tatarinowii Schott, Borneol, Moschus and other herbal medicine in stroke. The Conclusion is Chinese herbal medicine including Acorus tatarinowii Schott, Borneol and Moschus could reduce stroke injury and help brain recovery after stroke. More studies in Chinese herbal medicine and stroke may help us to develop a useful treatment for stroke. Keywords: Chinese herbal medicine, stroke, Acorus tatarinowii Schott, Borneol, Moschus ...
Chinese herbal medicine, stroke, Acorus tatarinowii Schott, Borneol, Moschus
Anti-anxiety Activity of Citrus paradisi var. duncan Extracts in Swiss Albino Mice -A Preclinical Study
Research Article of Journal of Herbal Medicine Research Anti-anxiety Activity of Citrus paradisi var. duncan Extracts in Swiss Albino Mice -A Preclinical Study Vikas Gupta1*, Parveen Bansal1, Junaid Niazi2, Kamlesh Kohli3, Pankaj Ghaiye4 1University Centre of Excellence in Research, BFUHS, Faridkot, India 2Department of Pharmaceutical Chemistry, Bahra Institute of Pharmacy, Patiala, India 3Department of Pharmacology, Guru Gobind Singh Medical College, BFUHS, Faridkot, India 4Department of Pharmaceutics, ACPTE, Sangrur, India Review method: Single-blind; Peer reviewer comments: 1. Background: Fragrances from aromatic oils of Citrus have been particularly attributed with mood enhancing properties by aroma therapists. Volatile oils are available in very small amounts hence authors intend to establish antianxiety activity of leaf extracts that can be made available for commercial purposes. Objectives: The present study was designed to authenticate the already established anti-anxiety activity (by using elevated plus maze model) of various extracts of the leaves of Citrus paradisi var. Duncan by authors using different animal models. Methods: Swiss Albino mice were treated with different doses of the leaf extracts (50, 100, 200 and 400 mg / kg p.o.) and Diazepam (2mg/kg, p.o) was used as a positive control. Antianxiety activity was determined using Y maze model, light dark model and hole board methods. Results: Results of study show that methanol extract in higher doses (100, 200, 400 mg/kg) possesses marked anti anxiety activity and was comparable to the effect produced by diazepam. Conclusion: The plant can be developed as a commercial source of anxiolytic agent. Further studies are in-process to isolate the active constituent responsible for this activity and mechanism of action. Keywords: Anxiety, Grapefruit, Hole board test, Light dark model, Y maze ...
Anxiety, Grapefruit, Hole board test, Light dark model, Y maze