Research Article of American Journal of Biotechnology and Bioinformatics
Preliminary Phytochemical Screening and In Vitro Anthelminthic Activity of Detarium Senegalense J.f Gmel (Fabaceae) Leaf Methanol Extract
1Ukwubile CA, 2Troy SM, 3 Ikpefan OE and 2Musa YD
1 Department of Science Laboratory Technology, Biology Unit, School of Science and Technology, Federal Polytechnic Bali, Nigeria.
2Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Nigeria.
3Department of Pharmacognosy and Herbal Medicine, Faculty of Pharmacy, Delta State University, Abraka, Nigeria.
Detarium senegalense is an importance medicinal plant used in North-east Nigeria and some African countries in traditional medicine. This present study evaluated the preliminary phytochemical screening and anthelminthic activity of methanol leaf extract. Phytochemical screening was carried out by solvent-solvent partitioning of leaf extract in chloroform, n-hexane, dioxane, ethyl acetate, acetone and methanol. The results showed that methanol fraction contained alkaloids, tannins, saponins, and cardiac glycosides while the ethyl acetate fraction contained only flavonoids with no anthracenes. Ethanol fraction demonstrated the highest anthelminthic activity with a decreased time of paralysis and death in the various groups of earthworm (Lumbricus terrestris) in dose-dependent manner at concentrations of 10 mg/mL, 20 mg/mL, 30 mg/mL, and 40 mg/mL. The time for paralysis and death for Group VII 400 mg/mL of extract were 5.0 ± 0.3min and 10.0 ± 1.02 min respectively. This result was compared with that of a standard anthelminthic drug piperazine citrate (USP) whose values were 12 ±1.01 min and 16 ± 1.02 min respectively for time of paralysis and death at p≤0.05 (one-way ANOVA). The results showed that D. senegalense leaf methanol extract possessed anthelminthic activities by osmotic shock at the clitellum portion of the earthworms, and this justifies the use of the plant extract in herbal medicine for the eradication of worms.
Keywords: Detarium senegalense, Phytochemicals, Anthelminthic, Earthworms, Piperazine citrate.
How to cite this article:
Ukwubile et al., Preliminary Phytochemical Screening and In Vitro Anthelminthic Activity of Detarium Senegalense J.f Gmel (Fabaceae) Leaf Methanol Extract. American Journal of Biotechnology and Bioinformatics, 2017; 1:2. DOI: 10.28933/ajobb-2017-09-2802
1. Victor,DS Elena. R.Mongelli.A, Antiparasitic activity of Meliaazedarach growing in Argtina, Molecular Medicinal Chemistry 1, 54-57, 2003.
2. Edwards.CA, Testing the effects of chemicals on earth worms. The advantages and limitations of field tests. Ecotoxicology of earth worms 1, 75-84, 1992.
3. Sollmann.T, Anthemintics: Their efficiency as tested on earth worms .J.Pharm ExpTher 12:129, 1918.
4. Adam, R.D., Thorne, G.w., Brawwald, E., et al. (1997). Phytochemical screening and in vitro anthelminthic activities of D. senegalense. Journal of medicinal microbiology, New York: 1088 – 1089.
5. Akah, P. Nworu, C., Mbaoji, F., et al. (2012). Anthelminthic evaluation of D. senegalense leaf extract. Journal of phytopharmacology, 3(2); 367 – 375.
6. Chaturerdi, M. Dwiredi, A. Barpete P. K., et al. (2009). Formulation and evaluation of polyherbal anthelminthic preparation. Journal of medical microbiology. 9(13) 329 – 331.
7. Cisse, M. I, Dieme, O. Diop, N., et al. (2010). principales Caraceristique et utilization au Senegal fruit. Journal of medical microbiology; 65(5), 293 – 306.
8. Coles, G. C., Rush, R.T (1992). Showing the spread of anthelminthic resistant nemstode sheep and goats in the United Kingdom. Journal of Veterinary parasitology.
9. Dash, G.K, Suresh, P.Kar et al., (2007). Evaluation of evolulus alsonoids Linn for anthelminthic and antimicrobial activities J. Nat. Rem., 182 – 185.
10. El- kamali,H. (2011). D.senegalense J.F Gmel, R. Lemmens, D. louppe and A. Oteng- Amoako (Eds), protabase, Wageningen Netherlands.
11. Gert, S. Dorny, P (1995). Anthelminthic resistance in helminth animal of man in the tropical Bulletindes- Seances; Academic-Royale-des-Science SD, 3:401-424.
12. Kamal, (2006). Effect of helminthes on the immune. Journal of medical microbiology. 11(3): 485 – 487.
13. Evans, W.C. (2016). Trease and Evans Pharmacognosy 16th Edition. Saunders Elsevier. Pp.54.
14. Sofowora, A.B. (2006). Medicinal Plants of West Tropical Africa. Spectrum Books Ibadan. Pp. 34.
15. Rook, Gaw, (2008). Review series on helminthes immune modulation and hygiene hypothesis, the breeder implication of the hygiene hypothesis. Journal of immunology. 126(1): 1365 – 2567.
16. Ukwubile, C.A et al. (2016). In vitro anthelminthic resistance activity of Pseudocedrella kotchsyii. International Biological and Biomedical Journal; 3: 12-17
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