Research Article of American Journal of Biotechnology and Bioinformatics
The intravarietal relationship among different accessions of Dolichos biflorus was checked based on protein profiling using SDS-PAGE
Smita Srivastava, Prem Kumar Dantu
Plant Biotechnology Laboratory, Department of Botany, Dayalbagh Educational Institute (Deemed University) Dayalbagh, Agra, India
In this study, the phylogenetic relationship within the selected Eleven Indian (Dolichos biflorus (horse gram) varieties was analyzed for total soluble seed protein. Twenty-five bands were documented through SDS PAGE based on 100 seed weight of each variety and were studied for genetic diversity. Jaccard’s similarity matrix was acquired and used in UPGMA cluster analysis based on the polymorphism generated by the presence (1) or absence (0) of protein bands. Thus, the dendrogram showed four major groups that corre-spond to an earlier study on polymorphisms of 11 accessions of Indian Dolichos. Signifi-cant correspondence between the clustering pattern and the pedigree was observed; thus, a high genetic diversity could be kept within the Dolichos varieties. A similarity matrix among the targeted genotypes and phylogenetic analysis is considered a unique feature in the present work. Therefore, the current investigation was carried out to analyse Protein diversity of unexplored Dolichos genotypes at the molecular level, construct a dendro-gram based on similarity band matrix and generate efficiency in genetic divergence analy-sis among Dolichos. This study underlines the importance of using genetic diversity based in the Dolichos breeding program.
Keywords: Cluster analysis; Dolichos biflorus; Dendrogram; Seed proteins; SDS-PAGE;
How to cite this article:
Smita Srivastava and Prem Kumar Dantu. The intravarietal relationship among different accessions of Dolichos biflorus was checked based on protein profiling using SDS-PAGE. American Journal of Biotechnology and Bioinformatics, 2021; 4:9. DOI: 10.28933/ajobb-2020-12-2505
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