THE INTRAVARIETAL RELATIONSHIP AMONG DIFFERENT ACCESSIONS OF DOLICHOS BIFLORUS WAS CHECKED BASED ON PROTEIN PROFILING USING SDS-PAGE


The intravarietal relationship among different accessions of Dolichos biflorus was checked based on protein profiling using SDS-PAGE


Smita Srivastava, Prem Kumar Dantu

Plant Biotechnology Laboratory, Department of Botany, Dayalbagh Educational Institute (Deemed University) Dayalbagh, Agra, India


American Journal of Biotechnology and Bioinformatics

In this study, the phylogenetic relationship within the selected Eleven Indian (Dolichos biflorus (horse gram) varieties was analyzed for total soluble seed protein. Twenty-five bands were documented through SDS PAGE based on 100 seed weight of each variety and were studied for genetic diversity. Jaccard’s similarity matrix was acquired and used in UPGMA cluster analysis based on the polymorphism generated by the presence (1) or absence (0) of protein bands. Thus, the dendrogram showed four major groups that corre-spond to an earlier study on polymorphisms of 11 accessions of Indian Dolichos. Signifi-cant correspondence between the clustering pattern and the pedigree was observed; thus, a high genetic diversity could be kept within the Dolichos varieties. A similarity matrix among the targeted genotypes and phylogenetic analysis is considered a unique feature in the present work. Therefore, the current investigation was carried out to analyse Protein diversity of unexplored  Dolichos   genotypes at the molecular level, construct a dendro-gram based on similarity band matrix and generate efficiency in genetic divergence analy-sis among  Dolichos. This study underlines the importance of using genetic diversity based in the Dolichos breeding program.


Keywords: Cluster analysis; Dolichos biflorus; Dendrogram; Seed proteins; SDS-PAGE;

Free Full-text PDF


How to cite this article:
Smita Srivastava and Prem Kumar Dantu. The intravarietal relationship among different accessions of Dolichos biflorus was checked based on protein profiling using SDS-PAGE. American Journal of Biotechnology and Bioinformatics, 2021; 4:9. DOI: 10.28933/ajobb-2020-12-2505


References:

1. Ayyangar, G. N. R., & Nambiar, K. K. K. (1935). Studies in Dolichos lablab (Roxb) and (L.). The Indian field and garden bean. In First Proceedings of Indian Academy of Science: 57–867.
2. Biju, M. G., Prasanna, K. P., & Rajan, S. (2001). Genetic divergence in Hyacinth bean. Vegetable Science, 28(2), 163–164.
3. Boghara, M. C., Dhaduk, H. L., Kumar, S., Parekh, M. J., Patel, N. J., & Sharma, R. (2016). Genetic divergence, path analysis and molecular diversity analysis in cluster bean (Cyamopsis tetragonoloba L. Taub.). Industrial Crops and Products, 89, 468-477.
4. Chahota, R.K. (2009). Induction of early flowering and other important traits in horse gram (Macrotyloma uniflorum) using gamma irradiation. Final project report submitted to Bhabha Atomic Research Centre, Trombay, India.
5. Chaitanya, V., Reddy, R. V. S. K., Pandravada, S., & Sujatha, M. (2013). Genetic divergence in dolichos bean (Dolichos lablab L. var. typicus) genotypes for yield and yield contributing traits. Asian J Hort, 8(2), 733-736.
6. Chaudhary, J., & Dantu, P. K. (2013). Phylogenetic relationships within selected Indian soybean (Glycine max (L.) Merr.) varieties based on SDS-PAGE of seed proteins. Journal of Proteins and Proteomics.
7. Duranti, M., Consonni, A., Magni, Ch., Sessa, F., & Scarafoni, A. (2008). The major proteins of lupin seed: Characterisation and molecular properties for use as functional and nutraceutical ingredients. Trends in Food Science and Technology, 19(12), 624–633. doi:10.1016/j.tifs.2008.07.002
8. Emare, I., & Slinkard, A. E. (2008). Electrophoresis analysis of some Lathyrus L. species based on seed storage protein. Biologia Plantarum, 39, 553–559.
9. Ghafoor, A., Ahmad, Z., Qureshi, A. S., & Bashir, M. (2002). Genetic relationship in Vigna mungo (L.). Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE. Euphytica, 123(3), 367–378. doi:10.1023/A:1015092502466
10. Griffin, J. D., & Palmer, R. G. (1995). Variability of thirteen isozymes loci in the USDA soybean germplasm collections. Crop Science, 35(3), 897–904. doi:10.2135/cropsci1995.0011183X003500030045x
11. Haider, N., Nabulsi, I., & MirAli, N. M. (2010). Comparison of the efficiency of A–PAGE and SDS– PAGE, ISSRs and RAPDs in resolving genetic relationships among Triticum and Aegilops species. Genetic Resources and Crop Evolution, 57(7), 1023–1039. doi:10.1007/s10722-010-9545-9
12. Hussain, A., Ramirez, H., & Bushuk. (1986). Field bean (Phaseolus vulgaris L.) cultivar identification by electrophoregrams of cotyledon seed storage. Euphytica, 35(729–732).
13. Iqbal, S. H., Ghafoor, A., & Ayub, N. (2005). Relationship between SDS-PAGE markers and Ascochyta blight in chickpea. Pakistan Journal of Botany, 37, 87–96.
14. Jha, S. S., & Ohri, D. (1996). Phylogenetic relationships of Cajanus cajan (L.) Millsp. (pigeon pea) and its wild relatives based on seed protein profiles. Genetic Resources and Crop Evolution, 43(3), 275–281. doi:10.1007/BF00123279
15. Karihaloo, J. L., Kaur, M., & Singh, S. (2002). Seed protein diversity in Solanum melongena L., and its wild and weedy relatives. Genetic Resources and Crop Evolution, 49(6), 533–539. doi:10.1023/A:1021288108928
16. Keerthi, C. M., Ramesh, S., Byregowda, M., & Vaijayanthi, P. V. (2018). Simple sequence repeat (SSR) Marker Assay-Based Genetic Diversity among Dolichos Bean (Lablab purpureus L. Sweet) Advanced Breeding Lines Differing for Productivity per se Traits. International Journal of Current Microbiology and Applied Sciences, 7(5), 3736–3744. doi:10.20546/ijcmas.2018.705.433
17. Kleim, P., Beavis, W., Schupp, J., & Freestone, R. (1992). ‘Evaluation of soybean RFLP marker density in adapted germplasm.’ Thoer. Journal of Applied Genetics, 85, 205–212.
18. Kuhar, K., Kansal, R., Subrahmanyam, B., Koundal, K. R., Miglani, K., & Gupta, V. K. (2013). A Bowman–Birk protease inhibitor with antifeedant and antifungal activity from Dolichos biflorus. Acta Physiologiae Plantarum. UK: Laemmli. Cleavage of structure proteins assembly of the head of bacteriophage T4.Nature, 35(6), 1887–1903. doi:10.1007/s11738-013-1227-8
19. Mandal, R. K., & Mandal, S. (2000). Seed storage proteins and approaches for improvement of their nutritional quality by genetic engineering. Current Science, 79, 576–589.
20. Masood, M. S., Asghar, M., & Anwar, R. A. S. H. I. D. (2004). Genetic diversity in wheat landraces from Pakistan based on polymorphism for high molecular weight glutenin subunits (HMW-GS). Pak. J. Bot, 36(4), 835-843.
21. Nagger, E. I. MCh. (2001). Implications of seed proteins in Brassicaceae systematic. Biologia Plantarum, 44, 457–533
22. Perry, M. C., & McIntosh, M. S. (1991). Geographical patterns of variation in the USDA soybean germplasm collections 1. Morphological traits. Crop Science, 31(5), 1350–1355. doi:10.2135/cropsci1991.0011183X003100050054x
23. Sachdeva, S., Bharadwaj, C., Sharma, V., Patil, B. S., Soren, K. R., Roorkiwal, M., . . . and Bhat, K. V. (2018). Molecular and phenotypic diversity among chickpea (Cicer arietinum) genotypes as a function of drought tolerance. Crop and Pasture Science, 69(2). doi:10.1071/CP17284
24. Sammour, R., Mustafa, A. E., Badr, S., & Tahr, W. (2007). Genetic variations in accessions of Lathyrus sativus L. Acta Botanica Croatica. 1–13.
25. Sodani, S. N., Paliwal, R. V., & Jain, L. K. (2006). Phenotypic stability for seed yield in rainfed horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. Arid Legumes for Sustainable Agriculture and Trade (Vol. 2), 4(53.336), 340.
26. Upadhyay, D., Mehta, N., Singh, J., & Sahu, M. (2012). Correlation and path analysis in Dolichos bean (Dolichos lablab L.).
27. Vavilov NI (1951). The origin variation immunity and breeding of cultivated plants (translation by K Star Chester). Chron Bot, 13, 1-364.
28. Yang, Y. X., Wu, W., Zheng, Y. L., Yang, W. T., & Cai, Q. R. (2008). Genetic diversity of storage proteins in cultivated buckwheat. Pakistan Journal of Biological Sciences, 11(13), 1662–1668. doi:10.3923/pjbs.2008.1662.1668
29. Zohary, D. (1970). Centres of diversity and centers of origin. Centres of diversity and centers of origin.