American Journal of Biotechnology and Bioscience

Enhanced Expression of Dermal Aquaporins by Polysaccharide from Camellia sinensis

The green tea polysaccharide was extracted from leaves of Camellia sinensis and hydrolyzed with enzyme. Skin moisturization effect of green tea polysaccharide was studied with 2D- PAGE analysis of epidermal hydration factors. Among up-regulated proteins extracted from Hacat cell treated with Green tea polysaccharide on 2DE gel, 5 aquaporins were observed. Among them the aquaporin 5 and 8 were increased 3.4 and 5.3 times respectively. The green tea polysaccharide was found to be effective for skin moisturization by enhanced expression of aquaporins.

Pre scaling up of bread wheat technology at Meket and wadla districts, North wollo zone

In Ethiopia where the livelihood of the people is highly dependent on agriculture improving the production and productivity of wheat will be one of the important agenda for the agricultural sector. The national average of wheat in the country, which is 14 Qt/ha, is 24%, is still below the average of South Africa yield and 48% below that of the world’s (FAO, 2004). Demonstration on farmers’ fields at Jama and Meket districts in 2014 cropping season showed that the improved variety sora had a better yield advantage over warkaye, Tossa and local with a better resistance to yellow rust. Having this pre scaling up of bread wheat variety sora with it full package had been started at Meket and wadla districts of north wollo zone, Amhara region. A total of 15 Qt seed had been provided for 25 farmers on 10 ha of land. Two clusters have been organized at the respective districts. 295 qt of seed had been produced and promoted for local seed system on established seed producer cooperatives. Farmers and development agents had invited to visit clustered farms through inviting on field days and experience had been shared. Thus further scaling up and out should be carried out on the study areas and similar agro ecological zones. Capacity building and continuous follow up should be done for the established cooperatives for sustaining seed production.

Performance of Sesame Variety in moisture-deficit areas of Wollo, Ethiopia

The field experiments was conducted at Kobo, Mersa and Chefa testing sites of Sirinka Agricultural Research Center in1999 and 2000 seasons for varieties adaptation and 2004 and 2005 seasons for genotypes selection. The experiments were conducted in randomized block design using three replications with the objectives of selecting adaptable variety for the lowland areas of Wollo. Analysis of variance for each environment revealed significant differences across the tested environments and from adaptation experiment variety Abasina is high seed-yielder (12.33 qt ha-1) and had low deviation from linear regression coefficient implying its stability for different environments. Therefore, Abasina was recommended for the lowland areas of northeastern Ethiopia and from genotype selection, Pungun yonggae showed stable performance and gave high seed and oil yield across the tested environments providing a seed and oil yield advantages of 99.0% and 94.9%, respectively over the standard check, while Kelafo 74 x C-22 sel 4 had specific adaptation to more favorable environments with a seed and oil yield advantages of 106.3 and 116.7%, respectively over the standard check. Therefore, based on their performances, Pungun yonggae and Kelafo 74 x C-22 sel 4 were officially released for production with the name of Borkena, and Ahadu respectively.

Demonstration of pearl millet technology at Raya kobo and Gubalafto districts, North wollo zone

Pearl millet [Pennisetum glaucum (L.) R.Br.] locally known as “Bajra” is a nutritious course grain cereal. Globally, it is grown on an area of 34.6 million ha with annual production of 28.8 million tons. Demonstration of improved variety kola 1 was conducted in 2016/17 cropping season at Gubalafto and Raya kobo districts to create demand on the pearl millet technology to farmers and development agents and thereby to assess their reactions towards the introduced technology. It was conducted on two FTC’s from kobo and Gubalafto districts. Though all agronomic practices were employed equally at both locations the performance of the variety varies significantly. The variety Kola 1 gave mean grain yield of 3.2 ton/ha and 1.8 ton/ha at Kobo and Hara respectively. The study indicated that cultivating pearl millet in moisture deficit areas can be an alternative for sustaining food security. Farmers have shown great interest and promised to adopt the technology from their neighbor farmers. Therefore technology promotions and popularization on the study and similar agro ecological areas should be carried out in collaboration with stake holders.

Development of Rainfall Runoff Modeling in Matlab Environment

The aim of this study is to understand the basic concept and parameters of rainfall-runoff model, and how that concept applies in model as well. And familiar with how to build/develop the models of rainfall-runoff, with more about the practical situations by considering corresponding different parameters according to the seasonal conditions (for example snowfall in winter season and its role according to the temperature), hydrological conditions of site also. Matlab is used as a tool for the developing this model. This type of models are very much useful to calculate the, how much water will enter in to deeper layers of sub-soil surfaces, when rainfall occurs and to calculate the total amount water losses in different forms (for example evaporation from land and transpiration from vegetation and trees) also. Get good experience while working with Matlab to develop the individual group model and while observing the already given scrip for example model to understand as well. While working with this exercise, got good questions about concept and parameters concerned for model development and had good discussion with in the working group and with available assistance/staff.

Dr. Jamunarani Veeraraghavan
Baylor College of Medicine, One Baylor Plaza, Houston, Texas

Dr. Moustafa M. Zeitoun
Professor, Department of Animal Production, Faculty of Agriculture, University of Alexandria, El-Shatby, Alexandria, Egypt; Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University

Dr. K.S. Kanwal
Scientist-C, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, North East Unit, Vivek Vihar, Itanagar-791113, Arunachal Pradesh, India

Dr. Surabhi Mishra
Resarch Scientist, Dept. of Microbiology, University of Iowa, IA – 52242

Dr. Miriam-Rose Menezes
UTHealth Science Center Houston

Dr. Yan Zhou
Harvard Medical School, Boston, USA

Dr. Richa Madan Lomash
Research Fellow, Receptor Biology Unit (NINDS), NIH, Bethesda (MD), USA

Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio, Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti (IT)

Dr. Sirisha Adamala
Assistant Professor, Department of Applied Engineering, Vignan’s University

Dr. Abhishek Naik
Area Manager, Technology development department

Dr. Moustafa Mohamed Sabry Bakry
Plant Protection Research Institute Agricultural Research Center, Giza, Egypt

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1.R. M. AKPLOGAN, G. H. T. CACAÏ, S. S. HOUEDJISSIN, V.MÈGNIKPA, C. AHANHANZ.Influence of mercuric chloride and sodium hypochlorite on apical and axillary buds regeneration of Colocasia esculenta in tissue culture. American Journal of Biotechnology and Bioscience, 2018; 2:10. DOI: 10.28933/ajbb-2018-11-1908 
2.Bienvenu T. Badou, Arnaud Agbidinoukoun, Gilles T.H. Cacaï, René C. Dossoukpèvi and Corneille Ahanhanzo. Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus). American Journal of Biotechnology and Bioscience, 2018; 2:9.. DOI: 10.28933/ajbb-2018-05-1801 

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American Journal of Biotechnology and Bioscience