American Journal of Biotechnology and Bioscience

Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus)

In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple’s homogeneous healthy planting materials.

Enhanced Expression of Dermal Aquaporins by Polysaccharide from Camellia sinensis

The green tea polysaccharide was extracted from leaves of Camellia sinensis and hydrolyzed with enzyme. Skin moisturization effect of green tea polysaccharide was studied with 2D- PAGE analysis of epidermal hydration factors. Among up-regulated proteins extracted from Hacat cell treated with Green tea polysaccharide on 2DE gel, 5 aquaporins were observed. Among them the aquaporin 5 and 8 were increased 3.4 and 5.3 times respectively. The green tea polysaccharide was found to be effective for skin moisturization by enhanced expression of aquaporins.

Pre scaling up of bread wheat technology at Meket and wadla districts, North wollo zone

In Ethiopia where the livelihood of the people is highly dependent on agriculture improving the production and productivity of wheat will be one of the important agenda for the agricultural sector. The national average of wheat in the country, which is 14 Qt/ha, is 24%, is still below the average of South Africa yield and 48% below that of the world’s (FAO, 2004). Demonstration on farmers’ fields at Jama and Meket districts in 2014 cropping season showed that the improved variety sora had a better yield advantage over warkaye, Tossa and local with a better resistance to yellow rust. Having this pre scaling up of bread wheat variety sora with it full package had been started at Meket and wadla districts of north wollo zone, Amhara region. A total of 15 Qt seed had been provided for 25 farmers on 10 ha of land. Two clusters have been organized at the respective districts. 295 qt of seed had been produced and promoted for local seed system on established seed producer cooperatives. Farmers and development agents had invited to visit clustered farms through inviting on field days and experience had been shared. Thus further scaling up and out should be carried out on the study areas and similar agro ecological zones. Capacity building and continuous follow up should be done for the established cooperatives for sustaining seed production.

Performance of Sesame Variety in moisture-deficit areas of Wollo, Ethiopia

The field experiments was conducted at Kobo, Mersa and Chefa testing sites of Sirinka Agricultural Research Center in1999 and 2000 seasons for varieties adaptation and 2004 and 2005 seasons for genotypes selection. The experiments were conducted in randomized block design using three replications with the objectives of selecting adaptable variety for the lowland areas of Wollo. Analysis of variance for each environment revealed significant differences across the tested environments and from adaptation experiment variety Abasina is high seed-yielder (12.33 qt ha-1) and had low deviation from linear regression coefficient implying its stability for different environments. Therefore, Abasina was recommended for the lowland areas of northeastern Ethiopia and from genotype selection, Pungun yonggae showed stable performance and gave high seed and oil yield across the tested environments providing a seed and oil yield advantages of 99.0% and 94.9%, respectively over the standard check, while Kelafo 74 x C-22 sel 4 had specific adaptation to more favorable environments with a seed and oil yield advantages of 106.3 and 116.7%, respectively over the standard check. Therefore, based on their performances, Pungun yonggae and Kelafo 74 x C-22 sel 4 were officially released for production with the name of Borkena, and Ahadu respectively.

Demonstration of pearl millet technology at Raya kobo and Gubalafto districts, North wollo zone

Pearl millet [Pennisetum glaucum (L.) R.Br.] locally known as “Bajra” is a nutritious course grain cereal. Globally, it is grown on an area of 34.6 million ha with annual production of 28.8 million tons. Demonstration of improved variety kola 1 was conducted in 2016/17 cropping season at Gubalafto and Raya kobo districts to create demand on the pearl millet technology to farmers and development agents and thereby to assess their reactions towards the introduced technology. It was conducted on two FTC’s from kobo and Gubalafto districts. Though all agronomic practices were employed equally at both locations the performance of the variety varies significantly. The variety Kola 1 gave mean grain yield of 3.2 ton/ha and 1.8 ton/ha at Kobo and Hara respectively. The study indicated that cultivating pearl millet in moisture deficit areas can be an alternative for sustaining food security. Farmers have shown great interest and promised to adopt the technology from their neighbor farmers. Therefore technology promotions and popularization on the study and similar agro ecological areas should be carried out in collaboration with stake holders.

Dr. Jamunarani Veeraraghavan
Baylor College of Medicine, One Baylor Plaza, Houston, Texas

Dr. Moustafa M. Zeitoun
Professor, Department of Animal Production, Faculty of Agriculture, University of Alexandria, El-Shatby, Alexandria, Egypt; Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University

Dr. K.S. Kanwal
Scientist-C, G.B. Pant National Institute of Himalayan Environment and Sustainable Development, North East Unit, Vivek Vihar, Itanagar-791113, Arunachal Pradesh, India

Dr. Surabhi Mishra
Resarch Scientist, Dept. of Microbiology, University of Iowa, IA – 52242

Dr. Miriam-Rose Menezes
UTHealth Science Center Houston

Dr. Yan Zhou
Harvard Medical School, Boston, USA

Dr. Richa Madan Lomash
Research Fellow, Receptor Biology Unit (NINDS), NIH, Bethesda (MD), USA

Department of Medical, Oral and Biotechnological Sciences, University G. d’Annunzio, Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti (IT)

Dr. Sirisha Adamala
Assistant Professor, Department of Applied Engineering, Vignan’s University

Dr. Abhishek Naik
Area Manager, Technology development department

Dr. Moustafa Mohamed Sabry Bakry
Plant Protection Research Institute Agricultural Research Center, Giza, Egypt

Manuscript Title: The title should be a brief phrase.

Author Information: List full names and affiliation of all authors, including Emails and phone numbers of corresponding author.

Abstract: The abstract should be less than 500 words. Following abstract, a list of keywords and abbreviations should be added. The keywords should be no more than 10. Abbreviation are only used for non standard and long terms.

Introduction: The introduction should included a clear statement of current problems.

Materials and Methods: This section should be clearly described.

Results and discussion: Authors may put results and discussion into a single section or show them separately.

Acknowledgement: This section includes a brief acknowledgment of people, grant details, funds

References: References should be listed in a numbered citation order at the end of the manuscript. DOIs and links to referenced articles should be added if available. Abstracts and talks for conferences or papers not yet accepted should not be cited. Examples Published Papers: 

1.R. M. AKPLOGAN, G. H. T. CACAÏ, S. S. HOUEDJISSIN, V.MÈGNIKPA, C. AHANHANZ.Influence of mercuric chloride and sodium hypochlorite on apical and axillary buds regeneration of Colocasia esculenta in tissue culture. American Journal of Biotechnology and Bioscience, 2018; 2:10. DOI: 10.28933/ajbb-2018-11-1908 
2.Bienvenu T. Badou, Arnaud Agbidinoukoun, Gilles T.H. Cacaï, René C. Dossoukpèvi and Corneille Ahanhanzo. Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus). American Journal of Biotechnology and Bioscience, 2018; 2:9.. DOI: 10.28933/ajbb-2018-05-1801 

Tables and figures: Tables should be used at a minimum with a short descriptive title. The preferred file formats for Figures/Graphics are GIF, TIFF, JPEG or PowerPoint.

Publication fee: The authors will be contacted about the publication fee after a manuscript have been accepted.

Proofreading and Publication: A proof will be sent to the corresponding author before publication. Authors should carefully read the proof to avoid any errors and return the proof to the editorial office. Editorial office will publish the article shortly and send a notice to authors with the links of the paper.

Open Access
American Journal of Biotechnology and Bioscience is a peer reviewed open access journal publishing research manuscripts, review articles, case reports, editorials, letters to the editor in Biotechnology and Bioscience (Indexing information).

Peer Review
To ensure the quality of the publications, all submitted manuscripts will be peer-reviewed by invited experts in the field. The decisions of editors will be made based on the comments of the reviewers.

Rapid Publication
Time to first decision: within 2 days for initial decision without review, 18 days with review; Time to publication: Accepted articles will be published online within 2 days, and final corrected versions by authors will be accessible within 5 days.  More details....

Rapid Response Team
Please feel free to contact our rapid response team if you have any questions. Our customer representative will answer your questions shortly.

American Journal of Biotechnology and Bioscience