American Journal of Biotechnology and Bioscience


Influence of mercuric chloride and sodium hypochlorite on apical and axillary buds regeneration of Colocasia esculenta in tissue culture

Taro (Colocasia esculenta L. SCHOTT) is a staple food in many southern countries and an ancient starchy crop consumed by more than 400 million people. It is treated by diseases and pests that affect seed availability. Thus, the techniques of in vitro culture mostly used to overcome the problem of seeds production meet enormous difficulties of infection and necrosis of the explants. This study aims to determine the optimal use of mercuric chloride and sodium hypochlorite for disinfection of apical and axillary buds of taro. For this purpose, three doses of sodium hypochlorite (8%, 10% and 12%) and mercuric chloride (0.08%, 0.1% and 0.15%) were used with three immersion times ( 25 min, 30 min and 45 min) for sodium hypochlorite and (5min, 7min and 10min) for mercuric chloride. A binary logistic analysis was performed to understand or predict the effect of different doses of NaOCl and HgCl2 on the behavior of apical and axillary buds of taro. The results showed that 8% sodium hypochlorite with immersion time of 25 minutes is favorable for the disinfection of both apical and axillary explants of taro. For mercuric chloride, only the dose of 0.15% is effective for apical bud survival. The present study offers an opportunity to make available the seed of taro through the organogenesis of the species without any risk of infection.

Effects of system benzylaminopurine-adenine sulphate in combination with naphthalene acetic on in vitro regeneration and proliferation of pineapple (Ananas comosus (L.) Mill var. comosus)

In vitro micropropagation is now used in the production of healthy and homogenous pineapple planting materials. In order to increase the in vitro proliferation of pineapple, the synergetic effect of adenine sulphate (AdS) and benzylaminopurine (BAP) in presence or not of naphthalene acetic acid (NAA) was evaluated. Median axillary buds of crowns bursted 6 to 8 weeks old were cultured on Murashige and Skoog medium supplemented with BAP (0, 1 and 2 mg/l), NAA (0 and 0.5 mg/l) with or without AdS (40 mg/l). Five buds per treatment with four replicates were used. The addition of BAP to the medium stimulated regeneration (65 to 82%) and multiplication (3.04 to 6.13 plantlets/explant) which were further enhanced by the addition of NAA (98%; 6.42 plantlets/explant). AdS highly stimulated proliferation in presence of 2 mg/l BAP and NAA (14.96 plantlets/explant), plantlet mass (1.143g) but limited plantlet growth in height which was more induced in the absence of regulators (4.88 cm) and with AdS alone (5.63 cm). Thus, BAP is necessary for the proliferation of pineapple and the addition of 0.5 mg/l NAA to 2 mg/l BAP and 40 mg/l AdS optimizes the multiplication and growth parameters of pineapple plantlets. These results allow the mass propagation of pineapple’s homogeneous healthy planting materials.

Enhanced Expression of Dermal Aquaporins by Polysaccharide from Camellia sinensis

The green tea polysaccharide was extracted from leaves of Camellia sinensis and hydrolyzed with enzyme. Skin moisturization effect of green tea polysaccharide was studied with 2D- PAGE analysis of epidermal hydration factors. Among up-regulated proteins extracted from Hacat cell treated with Green tea polysaccharide on 2DE gel, 5 aquaporins were observed. Among them the aquaporin 5 and 8 were increased 3.4 and 5.3 times respectively. The green tea polysaccharide was found to be effective for skin moisturization by enhanced expression of aquaporins.

Pre scaling up of bread wheat technology at Meket and wadla districts, North wollo zone

In Ethiopia where the livelihood of the people is highly dependent on agriculture improving the production and productivity of wheat will be one of the important agenda for the agricultural sector. The national average of wheat in the country, which is 14 Qt/ha, is 24%, is still below the average of South Africa yield and 48% below that of the world’s (FAO, 2004). Demonstration on farmers’ fields at Jama and Meket districts in 2014 cropping season showed that the improved variety sora had a better yield advantage over warkaye, Tossa and local with a better resistance to yellow rust. Having this pre scaling up of bread wheat variety sora with it full package had been started at Meket and wadla districts of north wollo zone, Amhara region. A total of 15 Qt seed had been provided for 25 farmers on 10 ha of land. Two clusters have been organized at the respective districts. 295 qt of seed had been produced and promoted for local seed system on established seed producer cooperatives. Farmers and development agents had invited to visit clustered farms through inviting on field days and experience had been shared. Thus further scaling up and out should be carried out on the study areas and similar agro ecological zones. Capacity building and continuous follow up should be done for the established cooperatives for sustaining seed production.

Performance of Sesame Variety in moisture-deficit areas of Wollo, Ethiopia

The field experiments was conducted at Kobo, Mersa and Chefa testing sites of Sirinka Agricultural Research Center in1999 and 2000 seasons for varieties adaptation and 2004 and 2005 seasons for genotypes selection. The experiments were conducted in randomized block design using three replications with the objectives of selecting adaptable variety for the lowland areas of Wollo. Analysis of variance for each environment revealed significant differences across the tested environments and from adaptation experiment variety Abasina is high seed-yielder (12.33 qt ha-1) and had low deviation from linear regression coefficient implying its stability for different environments. Therefore, Abasina was recommended for the lowland areas of northeastern Ethiopia and from genotype selection, Pungun yonggae showed stable performance and gave high seed and oil yield across the tested environments providing a seed and oil yield advantages of 99.0% and 94.9%, respectively over the standard check, while Kelafo 74 x C-22 sel 4 had specific adaptation to more favorable environments with a seed and oil yield advantages of 106.3 and 116.7%, respectively over the standard check. Therefore, based on their performances, Pungun yonggae and Kelafo 74 x C-22 sel 4 were officially released for production with the name of Borkena, and Ahadu respectively.

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American Journal of Biotechnology and Bioscience

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