American Journal of Microbiology and Immunology


Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.

Research Article of American Journal of Microbiology and Immunology Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.  IBEH.N.ISAIAH1 (BMLS, AIMLS, MNAAS) 1Department of Medical Microbiology, University of Benin Teaching Hospital, PMB 111 2Department of Microbiology, Faculty of Life Science, University Of Benin, PMB 1154, Introduction: Symptomatic and Asymptomatic urinary tract infection in women is of great importance to determine the causal and treatment of bacteruria in young women which may lead to disease states. Aim: to determine the occurrence of asymptomatic bacteriuria in young women and its relationship with urinary tract infection Methods: A microbiological analysis of urine was done on 3600 young women in urban Benin metroplolis from July 2009 to July 2010, a total of 1105 microorganism were isolated from both Symptomatic and Asymptomatic young women. Results: From the 1105 isolates from the descending order of occurrences Escherichia coli, Kleibseilla spp, Enterococcus faecalis and Staphylococcus aureus with Acinobacter spp with the least occurrence. Escherichia coli was the commonest cause of Asymptomatic urinary tract infection with 31.6 % and Acinectobacter sp 9.5% with the least occurrence of Asymptomatic urinary tract infections. Conclusion: This study shows that there is a rise in asymptomatic urinary tract infection amongst young women in Benin City. Keywords: BacterIuria, UTI, Asymptomatic ...

Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR

Research Article of American Journal of Microbiology and Immunology Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR S. M. El-Berbawy*; S.M. Sayed*;El-Toukhy, E. I. ** and Amal, A. Megahed*** * Assiut Lab. (Bacteriology Department) ** AHRI (Biotechnology Depart.) Dokki *** Port Said Lab. (Bacteriology Depart.) This study aims to investigate the slime production of Coagulase negative staphylococci (CoNS) isolates by phenotypic method on Congo Red Agar plates (CRA) and Genotypic detection of icaA, icaD and mecA genes by polymerase chain reaction (PCR). Out of 105 milk samples obtained from clinical bovine mastitis, 101samples (96.2%) were positive for bacterial growth. CoNS isolates was detected in 20 isolates with a percentage of 19.8%. Their ability to form biofilm as one of the most important virulence factors of the organisms using Congo Red Agar (CRA) method was investigated in which 13 out of 17 CoNS isolates (76.47%) were found to be slime producers. By PCR, mecA gene was found in threeout of 6 CoNS isolates (50%). Also six (100%) and three (50%) isolates were positive for icaA gene and icaD gene, respectively. In addition one isolate out of the six CoNS isolates (16.67%) was positive for the presence of icaA, icaD and mecA genes and also has the ability to form biofilm. The in vitro activities of CoNS against 11 selected antimicrobial agents referred that the highest resistance rate of CoNS observed to Lincomycin (100%), followed by Cefotaxime (94.41%), Oxacillin (58.82%), Ampicillin (47.06%) and Penicillin (41.18%), while the highest rate of sensitivity observed to Enrofloxacin and Gentamicin (100%, for each), followed by Doxycycline (94.11%).Conclusion, the findings of the present study demonstrated the ability of CoNS isolated from bovine clinical mastitis to form biofilms. This must be considered as an alarming situation, ...

The Accumulation Of Cadmium In Corn At Different Levels Of Soil Ph

Research Article of American Journal of Microbiology and Immunology The Accumulation Of Cadmium In Corn (Zea Mays L.) At Different Levels Of Soil Ph Rabah S. Shareef*1,2, Awang Soh Mamat*3, Zakaria Wahab*4, Ibni Hajar Rukunudin*5 1 College of Education-Al-Qiam, University of Anbar, Iraq. 2 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 3 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 4 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 5 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. A pot experiment was carried out in the plastic greenhouse at the Institute of Sustainable Agrotechnology (INSAT), University Malaysia Perlis in Sungai Chuchuh, Perlis, Malaysia. During season of the cultivation of 2014 investigate the impact of soil pH and cadmium on growth of corn plant (Zea mays L.). Twenty five were arranged in factorial experiments according to the Complete Randomized Design (CRD), with three replicates. Five levels of soil pH were, pH4, pH5.2 (i.e., the original value), pH6, pH7 and pH8 and five levels of cadmium (Cd); Cd 1, Cd 2, Cd 3, Cd 4 and Cd 0 where the amounts (2, 4, 6, 8 mg. kg-1 soil and control treatment without add cadmium) are applied as CdCl2. Thus, the total numbers of pots were 75 pots. The results of this investigation revealed that; The decrease of soil pH led to significant effect to increase concentration of cadmium in root, stem, leaves and grain of corn, where the level of pH 5.2 gave highest concentrations of Cd compared with pH 8. the interactions between soil pH and cadmium led to increase of reduced the accumulation of cadmium in the corn, where it gave the interaction between pH 8 and Cd 0 lowest concentration of cadmium in root, stem, leaves and grain. Keywords: Accumulation, Cadmium, Corn, Soil pH, ...

A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

Research Article of American Journal of Microbiology and Immunology A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions:  E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords: Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2 ...

Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA)

Research Article of American Journal of Microbiology and Immunology Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA) Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) This descriptive study was done on 101 milk samples obtained from clinically mastitic dairy cows in Assiut Governorate, Egypt. Staphylococcus aureus (S. aureus) was the main causative agent of clinical mastitis (34.65%) followed by S. saprophyticus (10.89%), S. intermedius and S. epidermidis (8.91%, for each). The other causative agents (non Staph. Spp.) were identified. Sensitivity test of S. aureus isolates was performed against 11 antimicrobial agents, where found that 21 S. aureus strains (60%) were methicillin resistant S. aureus (MRSA). Ten MRSA strains were subjected for: I- slime-producing factor on Congo Red Agar (CRA) plates phenotypically, as 6 isolates (60%) were positive for slime production. II- PCR which was optimized targeting mecA, icaA and icaD genes, where 5 isolates (50%) were positive for mecA gene. Six isolates (60%) and 8 isolates (80%) were positive for icaA and icaD genes, respectively. Five strains (50%) were positive for both icaA and icaD genes. Also 3 strains (30%) were positive for all mecA, icaA and icaD genes. Conclusion, it was concluded that bovine staphylococcal mastitis was the most predominant issue where S. aureus was the main cause. Detection of mecA gene in S. aureus isolates indicating that several cases suffering from S. aureus mastitis have an MRSA problem. Genotypic determination of mecA gene proved the most reliable method for detection of methicillin-resistant S. aureus. The present work paid an attention to the 3 MRSA strains (30%) were positive to all tested genes rather than slime production as the worst isolated strains all over this study (multidrug resistant, slime producing as well as carrying mecA, icaA and icaD genes). In vitro Enrofloxacin, Gentamicin and Doxycycline the most effective drugs for Staph. spp. clinical mastitis and should be recommended for treatment of such cases of bovine mastitis.    Keywords: Cows, clinical mastitis, methicillin-resistant Staphylococcus aureus, ...

Dr. Aziza Mahrous Mohamed Amer
Dean and Professor of pharmacology Department of Pharmacology – Faculty of Veterinary Medicine, Cairo University.; Fellow of National Academy of Science and Technology 2016; Member of Drug Council Academy of Science and Technology 2016

Dr. Pongsak Rattanachaikunsopon
Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand

Dr. Ying-Yu Jin
Head of Fermentation Team, Center for Nutraceutical and Pharmaceutical Materials, Yongin, Korea; Research Professor, Department of Biomodulation, Myongji University, Yongin, Korea.

Dr. Helen Treichel
Professor, Researcher, Deputy Coordinator of Research and Graduate Studies and Coordinator of the Graduate Program, Environmental Science and Technology at Universidade Federal da Fronteira Sul (UFFS) – Campus Erechim

Dr. Liesel Brenda Gende
Professor of Food Microbiology and Food Chemistry, Department of Chemistry, Faculty of Natural Science, Mar del Plata University, Buenos Aires-Argentina. Researcher at CONICET (National Council of Scientific and Technical Research of Argentina). Fellow of the Research Center on Social Bees. Specialty in apicultural microbiology.

Dr. Akrum Hamdy 
Prof. Poultry Physiology, Department Head of Animal and Poultry Production, Faculty of Agriculture, Minia University, Egypt, Fellow Academy Scientific Research and Technology

Dr. Mohamed Hamed Mohamed Al-Agamy
Professor, Microbiology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Dr. Aditi Singh
Associate Professor, Amity Institute of Biotechnology, Amity Univesity Uttar Pradesh, Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow-226028.

Dr. Kantha Deivi Arunachalam 
Professor and Head, Centre for Environmental Nuclear Research, Directorate of Research & Virtual Education, SRM University, Chennai, Tamilnadu., India.

Profa. Dra. Fabiana América Souza 
Universidade de Pernambuco – UPE (Campus Mata Norte), Universidade Federal Rural de Pernambuco – UFRPE, Universidade do Minho – UMinho/Portugal, Fundação de Ensino Superior de Olinda – FUNESO.

Dr.  Parichat Phumkhachorn
Assistant Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, THAILAND

Dr. Bamidele RAHEEM 
Researcher, Department of Food and Environmental Sciences, University of Helsinki, Finland.

Dr. Iran ALEMZADEH
Professor, Chemical and Petroleum Engineering Department, Sharif University of Technology, P.O. Box 11155-9468, Tehran IRAN.

Dr. Younes Smani
Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, Infectious Diseases Research Group, University of Seville/CSIC/University Hospitals Virgen del Rocio and Virgen Macarena, Seville, Spain.

Dr. Surabhi Mishra
Resarch Scientist, Dept. of Microbiology, University of Iowa, IA – 52242

Dr. Elsayed Ibrahim Elagamy
Professor of Immunology, Department of Applied Medical Sciences, College of Community (Unaizah), Qassim University, Kingdom of Saudi Arabia.

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1.Ibeh Isaiah Nnanna. Elevated Serum Procalcitonin an Adjunct for Early detection of Infant Tuberculosis in Paediatric HIV/AIDS. American Journal of Microbiology and Immunology, 2016,1:8. DOI: 10.28933/nnanna-ajmi-10-2016 

2.Jamal Bayed Salim.,Ahmed K. Bolad, Hamid Suliman A.. andss Mujeeb A.Kabbashi. The effect of Transfer Factor as Immunotherapy comparing with the effect of BCG in Mice challenged with Mycobacterium tuberculosis. American Journal of Microbiology and Immunology, 2017; 2:1. DOI: 10.28933/ajmi-2017-02-2801 

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American Journal of Microbiology and Immunology

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