American Journal of Microbiology and Immunology


Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.

Research Article of American Journal of Microbiology and Immunology Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.  IBEH.N.ISAIAH1 (BMLS, AIMLS, MNAAS) 1Department of Medical Microbiology, University of Benin Teaching Hospital, PMB 111 2Department of Microbiology, Faculty of Life Science, University Of Benin, PMB 1154, Introduction: Symptomatic and Asymptomatic urinary tract infection in women is of great importance to determine the causal and treatment of bacteruria in young women which may lead to disease states. Aim: to determine the occurrence of asymptomatic bacteriuria in young women and its relationship with urinary tract infection Methods: A microbiological analysis of urine was done on 3600 young women in urban Benin metroplolis from July 2009 to July 2010, a total of 1105 microorganism were isolated from both Symptomatic and Asymptomatic young women. Results: From the 1105 isolates from the descending order of occurrences Escherichia coli, Kleibseilla spp, Enterococcus faecalis and Staphylococcus aureus with Acinobacter spp with the least occurrence. Escherichia coli was the commonest cause of Asymptomatic urinary tract infection with 31.6 % and Acinectobacter sp 9.5% with the least occurrence of Asymptomatic urinary tract infections. Conclusion: This study shows that there is a rise in asymptomatic urinary tract infection amongst young women in Benin City. Keywords: BacterIuria, UTI, Asymptomatic ...

Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR

Research Article of American Journal of Microbiology and Immunology Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR S. M. El-Berbawy*; S.M. Sayed*;El-Toukhy, E. I. ** and Amal, A. Megahed*** * Assiut Lab. (Bacteriology Department) ** AHRI (Biotechnology Depart.) Dokki *** Port Said Lab. (Bacteriology Depart.) This study aims to investigate the slime production of Coagulase negative staphylococci (CoNS) isolates by phenotypic method on Congo Red Agar plates (CRA) and Genotypic detection of icaA, icaD and mecA genes by polymerase chain reaction (PCR). Out of 105 milk samples obtained from clinical bovine mastitis, 101samples (96.2%) were positive for bacterial growth. CoNS isolates was detected in 20 isolates with a percentage of 19.8%. Their ability to form biofilm as one of the most important virulence factors of the organisms using Congo Red Agar (CRA) method was investigated in which 13 out of 17 CoNS isolates (76.47%) were found to be slime producers. By PCR, mecA gene was found in threeout of 6 CoNS isolates (50%). Also six (100%) and three (50%) isolates were positive for icaA gene and icaD gene, respectively. In addition one isolate out of the six CoNS isolates (16.67%) was positive for the presence of icaA, icaD and mecA genes and also has the ability to form biofilm. The in vitro activities of CoNS against 11 selected antimicrobial agents referred that the highest resistance rate of CoNS observed to Lincomycin (100%), followed by Cefotaxime (94.41%), Oxacillin (58.82%), Ampicillin (47.06%) and Penicillin (41.18%), while the highest rate of sensitivity observed to Enrofloxacin and Gentamicin (100%, for each), followed by Doxycycline (94.11%).Conclusion, the findings of the present study demonstrated the ability of CoNS isolated from bovine clinical mastitis to form biofilms. This must be considered as an alarming situation, ...

The Accumulation Of Cadmium In Corn At Different Levels Of Soil Ph

Research Article of American Journal of Microbiology and Immunology The Accumulation Of Cadmium In Corn (Zea Mays L.) At Different Levels Of Soil Ph Rabah S. Shareef*1,2, Awang Soh Mamat*3, Zakaria Wahab*4, Ibni Hajar Rukunudin*5 1 College of Education-Al-Qiam, University of Anbar, Iraq. 2 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 3 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 4 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 5 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. A pot experiment was carried out in the plastic greenhouse at the Institute of Sustainable Agrotechnology (INSAT), University Malaysia Perlis in Sungai Chuchuh, Perlis, Malaysia. During season of the cultivation of 2014 investigate the impact of soil pH and cadmium on growth of corn plant (Zea mays L.). Twenty five were arranged in factorial experiments according to the Complete Randomized Design (CRD), with three replicates. Five levels of soil pH were, pH4, pH5.2 (i.e., the original value), pH6, pH7 and pH8 and five levels of cadmium (Cd); Cd 1, Cd 2, Cd 3, Cd 4 and Cd 0 where the amounts (2, 4, 6, 8 mg. kg-1 soil and control treatment without add cadmium) are applied as CdCl2. Thus, the total numbers of pots were 75 pots. The results of this investigation revealed that; The decrease of soil pH led to significant effect to increase concentration of cadmium in root, stem, leaves and grain of corn, where the level of pH 5.2 gave highest concentrations of Cd compared with pH 8. the interactions between soil pH and cadmium led to increase of reduced the accumulation of cadmium in the corn, where it gave the interaction between pH 8 and Cd 0 lowest concentration of cadmium in root, stem, leaves and grain. Keywords: Accumulation, Cadmium, Corn, Soil pH, ...

A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

Research Article of American Journal of Microbiology and Immunology A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions:  E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords: Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2 ...

Manuscript Title: The title should be a brief phrase.

Author Information: List full names and affiliation of all authors, including Emails and phone numbers of corresponding author.

Abstract: The abstract should be less than 500 words. Following abstract, a list of keywords and abbreviations should be added. The keywords should be no more than 10. Abbreviation are only used for non standard and long terms.

Introduction: The introduction should included a clear statement of current problems.

Materials and Methods: This section should be clearly described.

Results and discussion: Authors may put results and discussion into a single section or show them separately.

Acknowledgement: This section includes a brief acknowledgment of people, grant details, funds

References: References should be listed in a numbered citation order at the end of the manuscript. DOIs and links to referenced articles should be added if available. Abstracts and talks for conferences or papers not yet accepted should not be cited. Examples Published Papers: 

1. Hongmei YANG. A study on the Management Mode of Modern Vocational Education in China. Global journal of Economics and Business Administration, 2018, 3: 10.DOI: 10.28933/gjeba-2018-07-2301 

Tables and figures: Tables should be used at a minimum with a short descriptive title. The preferred file formats for Figures/Graphics are GIF, TIFF, JPEG or PowerPoint.

Publication fees: We do not charge any submission charges, but authors are required to pay publication fees after their manuscripts are accepted for publication. For authors who receive our invitations, please send us the invitation code to receive the special discount.

Proofreading and Publication: A proof will be sent to the corresponding author before publication. Authors should carefully read the proof to avoid any errors and return the proof to the editorial office. Editorial office will publish the article shortly.

Withdrawal Policy: When you want to withdraw an article, please contact us. We will withdraw/retract it from our system after reviewing your request.

Terms of Use/Privacy Policy/Disclaimer/Other Policies: you agree that by using or receiving our services, you have read, understood, and agreed to be bound by all of our terms of use/privacy policy/ disclaimer/ other policies.

About the journal
The journal is hosted by eSciPub LLC. Our aim is to provide a platform that encourages publication of the most recent research and reviews for authors from all countries.


About the publisher
eSciPub LLC is a publisher to support Open Access initiative located in Houston, Texas, USA. It is a member of the largest community of professional publishers in the United States: the Independent Book Publishers Association. It hosts more than 100 Open Access journals in Medicine, Business & Economics, Agriculture, Biological Sciences, Chemistry, Education, Physical Sciences, Sociology, and Engineering and Technology.


Rapid Response Team
Please feel free to contact our rapid response team if you have any questions. Our customer representative will answer your questions shortly.

American Journal of Microbiology and Immunology

Loading