American Journal of Microbiology and Immunology


A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

Research Article of American Journal of Microbiology and Immunology A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions:  E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords: Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2 ...

Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA)

Research Article of American Journal of Microbiology and Immunology Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA) Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) This descriptive study was done on 101 milk samples obtained from clinically mastitic dairy cows in Assiut Governorate, Egypt. Staphylococcus aureus (S. aureus) was the main causative agent of clinical mastitis (34.65%) followed by S. saprophyticus (10.89%), S. intermedius and S. epidermidis (8.91%, for each). The other causative agents (non Staph. Spp.) were identified. Sensitivity test of S. aureus isolates was performed against 11 antimicrobial agents, where found that 21 S. aureus strains (60%) were methicillin resistant S. aureus (MRSA). Ten MRSA strains were subjected for: I- slime-producing factor on Congo Red Agar (CRA) plates phenotypically, as 6 isolates (60%) were positive for slime production. II- PCR which was optimized targeting mecA, icaA and icaD genes, where 5 isolates (50%) were positive for mecA gene. Six isolates (60%) and 8 isolates (80%) were positive for icaA and icaD genes, respectively. Five strains (50%) were positive for both icaA and icaD genes. Also 3 strains (30%) were positive for all mecA, icaA and icaD genes. Conclusion, it was concluded that bovine staphylococcal mastitis was the most predominant issue where S. aureus was the main cause. Detection of mecA gene in S. aureus isolates indicating that several cases suffering from S. aureus mastitis have an MRSA problem. Genotypic determination of mecA gene proved the most reliable method for detection of methicillin-resistant S. aureus. The present work paid an attention to the 3 MRSA strains (30%) were positive to all tested genes rather than slime production as the worst isolated strains all over this study (multidrug resistant, slime producing as well as carrying mecA, icaA and icaD genes). In vitro Enrofloxacin, Gentamicin and Doxycycline the most effective drugs for Staph. spp. clinical mastitis and should be recommended for treatment of such cases of bovine mastitis.    Keywords: Cows, clinical mastitis, methicillin-resistant Staphylococcus aureus, ...

Dr. Aziza Mahrous Mohamed Amer
Dean and Professor of pharmacology Department of Pharmacology – Faculty of Veterinary Medicine, Cairo University.; Fellow of National Academy of Science and Technology 2016; Member of Drug Council Academy of Science and Technology 2016

Dr. Pongsak Rattanachaikunsopon
Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand

Dr. Ying-Yu Jin
Head of Fermentation Team, Center for Nutraceutical and Pharmaceutical Materials, Yongin, Korea; Research Professor, Department of Biomodulation, Myongji University, Yongin, Korea.

Dr. Helen Treichel
Professor, Researcher, Deputy Coordinator of Research and Graduate Studies and Coordinator of the Graduate Program, Environmental Science and Technology at Universidade Federal da Fronteira Sul (UFFS) – Campus Erechim

Dr. Liesel Brenda Gende
Professor of Food Microbiology and Food Chemistry, Department of Chemistry, Faculty of Natural Science, Mar del Plata University, Buenos Aires-Argentina. Researcher at CONICET (National Council of Scientific and Technical Research of Argentina). Fellow of the Research Center on Social Bees. Specialty in apicultural microbiology.

Dr. Akrum Hamdy 
Prof. Poultry Physiology, Department Head of Animal and Poultry Production, Faculty of Agriculture, Minia University, Egypt, Fellow Academy Scientific Research and Technology

Dr. Mohamed Hamed Mohamed Al-Agamy
Professor, Microbiology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Dr. Aditi Singh
Associate Professor, Amity Institute of Biotechnology, Amity Univesity Uttar Pradesh, Lucknow Campus, Malhaur, Gomti Nagar Extension, Lucknow-226028.

Dr. Kantha Deivi Arunachalam 
Professor and Head, Centre for Environmental Nuclear Research, Directorate of Research & Virtual Education, SRM University, Chennai, Tamilnadu., India.

Profa. Dra. Fabiana América Souza 
Universidade de Pernambuco – UPE (Campus Mata Norte), Universidade Federal Rural de Pernambuco – UFRPE, Universidade do Minho – UMinho/Portugal, Fundação de Ensino Superior de Olinda – FUNESO.

Dr.  Parichat Phumkhachorn
Assistant Professor, Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani 34190, THAILAND

Dr. Bamidele RAHEEM 
Researcher, Department of Food and Environmental Sciences, University of Helsinki, Finland.

Dr. Iran ALEMZADEH
Professor, Chemical and Petroleum Engineering Department, Sharif University of Technology, P.O. Box 11155-9468, Tehran IRAN.

Dr. Younes Smani
Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, Infectious Diseases Research Group, University of Seville/CSIC/University Hospitals Virgen del Rocio and Virgen Macarena, Seville, Spain.

Dr. Surabhi Mishra
Resarch Scientist, Dept. of Microbiology, University of Iowa, IA – 52242

Dr. Elsayed Ibrahim Elagamy
Professor of Immunology, Department of Applied Medical Sciences, College of Community (Unaizah), Qassim University, Kingdom of Saudi Arabia.

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1.Ibeh Isaiah Nnanna. Elevated Serum Procalcitonin an Adjunct for Early detection of Infant Tuberculosis in Paediatric HIV/AIDS. American Journal of Microbiology and Immunology, 2016,1:8. DOI: 10.28933/nnanna-ajmi-10-2016 

2.Jamal Bayed Salim.,Ahmed K. Bolad, Hamid Suliman A.. andss Mujeeb A.Kabbashi. The effect of Transfer Factor as Immunotherapy comparing with the effect of BCG in Mice challenged with Mycobacterium tuberculosis. American Journal of Microbiology and Immunology, 2017; 2:1. DOI: 10.28933/ajmi-2017-02-2801 

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American Journal of Microbiology and Immunology

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