Performance, Haematology And Serum Biochemical Parameters Of Growing Grass Cutters (Thyronoyms Swinderianus) Fed Phyllantus Amarus And Pilogstigma Thonngii Leaf Meal Mixture As Partial Replacement For Soya Bean Meal

Performance, Haematology And Serum Biochemical Parameters Of Growing Grass Cutters (Thyronoyms Swinderianus) Fed Phyllantus Amarus And Pilogstigma Thonngii Leaf Meal Mixture As Partial Replacement For Soya Bean Meal

1*Alagbe, J.O., 2 Agubosi, O.C.P and 3 Liu, S.D

1, 3 Sumitra Research Institute 2 University of Abuja

The objective of the present study was to evaluate the performance, haematology and se-rum biochemical parameters of growing grasscutters (Thrynomys swinderianus) fed Phyl-lantus amarus (PAM) and Pilogstigma thonngii leaf meal (PTM) mixture as partial re-placement for soya bean meal. A total of 35 weaned grasscutters of mixed sex between 5-6 weeks with an average weight of 436.1 and 437.0 g were divided into five groups of 7 animals each and randomly assigned to 5 experimental diets each animal served as a repli-cate in a completely randomized design. The dietary treatments include a control diet with no PATML, T2 (10.0% PATML), T3 (20.0 % PATML), T4 (30.0 % PATML) and T5 (40.0 % PATML) respectively. Feed and water were offered ad libitum throughout the experiment which lasted for 12 weeks. The data obtained was used to evaluate the growth, haematological parameters (PCV, RBC, Hb, MCV, MCH , MCHC, WBC and its differentials ), serum biochemical indices (Albumin, globulin, total protein, creatinine, cholesterol, calcium, phosphorus, ALP, AST and ALT) and fatty acid composition. Aver-age weight gain, average daily feed intake and feed conversion ratio were influenced by the dietary treatments (P<0.05). Haematological and serum parameters were significantly (P<0.05) different among the treatments and were within the normal physiological range for grasscutters. It could be concluded that replacement of soya meal with 40 % PATML does not have any deleterious effect on the performance and blood profile of the animal.

Keywords: Weaner grasscutters; Phyllantus amarus; Pilogstigma thonngii; haematology

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Alagbe, J.O., Agubosi, O.C.P and Liu, S.D. Performance, Haematology And Serum Biochemical Parameters Of Growing Grass Cutters (Thyronoyms Swinderianus) Fed Phyllantus Amarus And Pilogstigma Thonngii Leaf Meal Mixture As Partial Replacement For Soya Bean Meal. Advances in Research and Reviews, 2020, 1:13


1. Adeniji, A.A. (2009). Protein and energy require-ments of weaner grasscutters. Animal Nutrition and Feed Technology, 9(1):73-79.
2. Allan, L and Miller, N.D. (1996). Antioxidant, fla-vonoids structure, function and clinical usage. Alternative Medicine and Research, 1:320-329.
3. Alagbe, J.O. (2020). Performance, haematology and serum biochemical parameters of weaner rabbits fed different levels of fermented lagenar-ia brevlifora whole fruit extract. Advances in Re-search and Reviews, 1(5):1-12.
4. Alagbe, J.O., Sharma, D.O and Xing, Liu. (2019). Effect of aqueous Piliostigma thonningii extracts on the haematological and serum biochemical indices of broiler chicken. Noble International Journal of Agriculture and Food Technology, 1(2):62-69.
5. Ajibade, V.A and Fagbohun, E.D.(2010).Physicochemical, Proximate Analysis and Antimicrobial Activities of Methanolic Crude Extract of Tylophora glauca (Bullock.) Journal of Pharmaceutical and Biomedical Sciences, 1(1):7-12.
6. Byanet, O., Adamu, S., Salami, S.O and Obadi-ah, H.I. (2008). Haematological and plasma bio-chemical parameters of the young grasscutter reared in the northern Nigeria. Journal of Cell and Animal Biology, 2(10): 177-181.
7. Ogunsanmi, A.O., Ozegbe, P.C., Ogunjobi, O., Taiwo, V.O and Adu, J.O. (2002). Haematologi-cal, plasma biochemistry and whole blood min-erals of the captive adult African grasscutter. Tropical Veterinarian, 20(1):97-100
8. Opara, M.N., Ike, K.A and Okoli, I.C. (2006). Haematology and plasma biochemistry of the wild adult African grasscutter. Journal of Animal Science, 2(2): 17-22.
9. Ighodaro, I., Agunbiade, S.O., Omale, J.O and Kuti, O.A. (2012). Evaluation of the chemical nu-tritional, antimicrobial and antioxidant vitamin profiles of Piliostigma thonningii leaves. Re-search Journal of medicinal plants 6: 537-543.
10. Alagbe, J.O. (2016). Nutritional evaluation of sweet orange (Citrus sinensis) fruit peel as re-placement for maize in the diet of weaner grass cutters. Scholarly Journal of Agricultural Sci-ence, 6(8): 277-282.
11. Alagbe, J.O and Omokore, E.A. (2019). Effect of replacing soya bean meal with Indigofera zollingeriana leaf meal on the performance and carcass characteristics of growing rabbits. Inter-national Journal of Multidisciplinary Research and Development, 6 (5): 74-77.
12. Asibey, E.O.A. (1974).Wildlife as a source of protein in Africa South of the Sahara. Biological Conservation 6:32-39.
13. Omokore, E.O and Alagbe, J.O. (2019). Efficacy of dried Phyllantus amarus leaf meal as an herb-al feed additive on the growth performance, haematological and serum biochemistry of grow-ing rabbits. International Journal of Academic Research and Development, 4(3):97-104.
14. Adeolu, A.A and Sunday, O.O (2013). Anti-inflammatory and analgesics activities of soft drink extract of Phyllantus amarus in some la-boratory animals. British Biotechnology Journal, 3: 191-204.
15. Meduna, A. J. (2002). Preliminary observations on cane rat feeding and breeding of the Federal College of Forestry, Ibadan. Proceeding of the 27th Annual Conference on Nigerian Society of Animal Production (FUTA), pp 304-305.
16. Wogar, G.S.I and Agwunobi, L.N. (2011). Per-formance and energy requirements for gestating grasscutters fed Agro-industrial by products. Journal of Agricultural Sciences, 4(3): 275-280.
17. Alagbe, J.O and Oluwafemi, R. A. (2019). Per-formance and haematological parameters of broiler chicks given different level of dried lemon grass and garlic extract. Research in: Agricultural and Veterinary Sciences, 3(2): 102-111.
18. Akinpelu, D. A and Obuotor, E.M. (2000).Antibacterial activity of Piliostigma thon-ningii stem bark. Fitoterapia 71: 442-443.
19. Butterworth, A.E (1999). Cell mediated damage in helminthes. Advanced Journal of Parasitology 23:143.
20. Frankič, T., Volje, M., Salobir, I and Rezar, V. (2009). Use of herbs and spices and their ex-tracts in animal nutrition. Acta Agric. Slov. 94:95-102.
21. Gotoh, S., Takennako, O., Vatanabe, K., Kawa-moto, R and Watanabe, T. (2001). Hematological values and parasitic fauna in free ranging Macaca hecki and the Macaca tonkeanai hecki hybrid group of Salawesi Island. Indonesia Pri-mates 6:91-100.
22. Alagbe, J.O. (2013). Performance and blood profile of grasscutters (Thryonomys swinderia-nus) fed wild sunflower leaf meal. International Journal of Science and Research, 5(6): 124-128.
23. A.O.A.C.(2000). Association of Official Analyti-cal Chemists. Official Methods of Analysis 19th Edition Washington, D.C Pages 69-77.
24. Duncan, D.B. (1955). Multiple range and multiple F-test. Biometrics 11(1):1-42.
25. Alagbe, J.O. (2019). Performance and Haemato-Biochemical Parameters of Weaner Rabbits Fed Diets Supplemented with Dried Water Melon Peel (Rind) Meal. Journal of Dairy and Veterinary Sciences 8(4):001-007.
26. Tavares, F.L., Sousa, M.C.C., Santaro, M.L., Barbaro, K.C., Rebecchi, I.M.M and Santos-Martins, I.S. (2004). Changes in haematological, hemostatic and biochemical parameters induced experimentally in rabbits by Loxosceles gaucho spider venom. Journal of Human Experimental Toxicology, 23:477-486.
27. Burkill, H.M. (1994). The useful of West Tropical Africa. 2, 2nd Ed. Royal botanic.
28. Chandan, S., Umesha, S and Balamurugan, V. (2012). Anti leptospiral antioxidant and DNA damaging properties of Eclipta alba and Phyl-lantus amarus. Open Access Scientific Reports, 1(4):1-8.
29. Evi, P.L and Degbeku, K. (2011). Antidiabetic activity of Phyllantus amarus and Thonn on Alloxan induced diabetes in male wister rats. Journal of Applied Sciences 11(6): 2968-2973.
30. Isidorov, V.A., Lech, P., Zolciak, A and Szczep-aniak, L. (2008). Gas chromatographic mass spectrometric investigation of metabolites from the needles and roots of pine seedlings at early stages of pathogenic fungi Armillaria ostoyae at-tack. Trees, 22: 531-542.
31. Manikkoth, S., Dcepa, B., Joy, A.E and Rao, S. (2011). Anticonvulsant activity of Phyllantus am-arus in experimental animal models, 4:144-149.
32. Oluwafemi, F and Debiri, F. (2008). Antimicrobial effect of Phyllantus amarus and Parquetina ni-grescens on Salmonella typhi, African Journal of Biomedical Research, 11:215-219.
33. Omer, H.A.A., El-Nomeary, Y.A.A., El-kady, R.i., Badr, A.M.M., Ali, F.A.F., Ahmed, S and Ibrahim, S.A.M. (2012). Growth performance of rabbits fed diets containing different levels of energy and mixture of some medicinal plants. Journal of Agricultural Science, 4:201-212.
34. Khartoon, S., Rai, V and Rawat, A. (2004). Com-parative pharmacognostic studies of 3 Phyl-lantus spp. Journal of Ethnopharmacology, 104:79-86.
35. Rajeshkumar, N.V., Joy, K.L., Kuttan, G., Rame-sewak, R.S., Nair, M.G and Kuttan, R. (2002). Antitumor and anticarcinogenic activity of Phyl-lantus amarus extract. Journal of Ethnopharma-cology, 81(1):17-22.
36. Prasad, P.D., Kavimani, S., Suba, V., Nudu, T., Sanatorium, T and Nadu, T. (2013). Antimicrobial activity of the root extracts of Phyllantus ama-rus. Journal of Ethnopharmacology, 4(1):1039-1043.
37. Kim, J.E., Lillehoj, H.S., Hong, Y.H., Kim, G.B., Lee, S.H., Lillehoj, E.P and Bravo, D.M. (2015) Dietary Capsicum and Curcuma longa oleoresins increase intestinal microbiome and necrotic en-teritis in three commercial broiler breeds. Res Vet Sci 102:150–158
38. Sofowora, A. (1993). Medicinal plants and tradi-tional medicine. Spectrum Books Ltd, Ibadan, Nigeria, 224-227.
39. Tasheen, M and Mishra, G. (2013). Ethanobotany and diuretic activity of some selected Indian medicinal plants. The Pharma Innovation, 2:112.
40. Zhang, Z. (2014). Bufalin attenuates the stage and metastatic potential of hepatocellular carci-noma in nude mice. Journal of Traditional Medi-cine 12: 57-62.
41. Zubair, M.F., Atolani, O., Ibrahim, S.O., Adebisi, O.O., Hamid, A.A and Sowunmi, R.A. (2016). Chemical constituents and antimicrobial proper-ties of Phyllantus amarus. Bayero Journal of Pure and Applied Sciences, 10(1): 238-246.
42. Alagbe, J.O. (2018). Effect of different levels of dried Delonix regia seed meal on the perfor-mance, haematology and serum biochemistry of growing grasscutters. Agricultural Research and Technology Open Access Journal, 18(4): 12-18
43. Jimoh, F.O and Oladiji, A.T. (2005). Preliminary studies on Piliostigma thonningii seeds: Proxi-mate analysis, mineral composition and phyto-chemical screening. African Journal of Biotech-nology, 4(12): 1439-1442.
44. Oyagade, J.O., Awotoye, O.O., Adewumi, T.J and Thorpe, H.T. (1999). Antibacterial activity of some Nigerian medicinal plants screening. Jour-nal of Biomedical Research 11(3):193-197.
45. Nakayama, N.G., Lindsey, M.L and Michael, L.H. (1993). Inhibition of the infectivity of influenza vi-rus by tea polyphenoids. Antiviral Research 21:289-299.
46. Srinivasan, K. (2005). Spices as influencers of body metabolism: an overview of three decades of research. Journal of Food Research, 38:77-86.
47. Liu, Y., Song, M,, Che, TM., Bravo, D., Petti-grew, J.E. (2012) Anti‑inflammatory effects of several plant extracts on porcine alveolar mac-rophages in vitro. Journal of Animal Science, 90:2774–2783
48. Onu, M.M., Oluwafemi, F and Debiri, F (2004). Effect of graded levels of dietary penicillin on the growth rate and feed conversion of broiler chicks. Journal of Agriculture and Social Re-search, 4(2): 81-89.
49. Olatunji, A.K., Alagbe, J.O and Hammed, M.A. (2015). Effect of varying levels of Moringa olif-era leaf meal on performance and blood profile of weaner rabbits. International Journal of Sci-ence and Research, 5(6): 803-806.
50. Olanipekun, M.K., Adewuyi, D and Adedeji, D.E. (2016). Ethnobotanical importance and phyto-chemical analysis of some selected medicinal plants used in Ado-Ekiti Local Government Area, Ekiti State. Journal of Herbal Medicine Research, 1(3): 0007-0016.
51. Obikaonu, H.O., Okoli, I.C., Opara, M.N., Okoro, V.M.O., Ogbuewu, I.P., Etuk, E.B and Udedbie, A.B.I. (2011). Haematological and serum bio-chemical indices of starter broilers fed neem leaf meal. Online Journal of Animal and Feed Re-search, 1(4):150-154.
52. National Research Council (1994). Nutrient re-quirement of poultry 9th Rev Edn, Washington D.C. National Academy Press.
53. Alinnor, J and Oze, R.(2011). Chemical Evalua-tion of the Nutritive value of Pentaclethra macro-phylla benth (African Oil Bean) Seeds. Pakistan Journal of Nutrition, 10 (4):355 – 359.
54. Akpanyung, E.O. (2005). Proximate and mineral composition of bouillon cubes produced in Ni-geria. Pakistan Journal of Nutrition, 4(5): 327-329.
55. Ibrahim, N.D.G., Abdulrahman, E.M and Ibrahim, G. (2001). Elemental analysis of the leaves of Vernonia amydalina and its biological evaluation in rats. Nigerian Journal of Natural Products and Medicine, 5:13-17.
56. Ross, I.A. (2005). Medicinal plants of the world : chemical constituents, traditional and modern medicinal uses. Humma Press Inc. New Jersey 1: 15-31.
57. Lillehoj, H.S and Lee, K.W. (2012) Immune modu-lation of innate immunity as alternatives to anti-biotics strategies to mitigate the use of drugs in poultry production. Journal of Poultry Science, 91:1286–1291
58. Min, B.R., Hart, S.P., Miller, D., Tomita, G.M., Loetz, E and Sahlu, T. (2005) The effect of graz-ing forage containing condensed tannins on gas-tro‑intestinal parasite infection and milk compo-sition in Angora does. Journal of Veterinary Par-asitology, 130:105–113.
59. Wallace, R.J, Arthaud, L., Newbold, C.J. (1994) Influence of Yucca shidigera extract on ruminal ammonia concentrations and ruminal microor-ganisms. Applied Environmental Microbiology 60: 1762–1767.
60. Redondo, L.M., Chacana, P.A., Dominguez, J.E and Miyakawa, M.E.F. (2014) Perspectives in the use of tannins as alternative to antimicrobial growth promoter factors in poultry. Front Micro-biology, 5:118.
61. Hollman, P.C. (2001). Evidence for health bene-fits of plant phenols: Local or systemic effects. Journal of Food Science and Agriculture, 81: 842-852.
62. Faizi, S., Khan, R.A., Mughal, N.R., Malik, M.S., Sajjadi, K.E and Ahmad, A. (2008). Antimicrobial activity of various effect of Polyalthia longifolia : isolation of active principles from the leaves and barriers. Journal of Phytochemistry, 22: 907-912.
63. Ovuru, S.S and Ekweozor, I. K. E. (2004). Hae-matological changes associated with crude oil ingestion in experimental rabbits. African Journal of Biotechnology, 3(6):346-348.
64. Ojewuyi, O.B., Ajiboye, T.O., Adebanjo, E.O., Balogun, A and Mohammed, A.O. (2014). Proxi-mate composition, phytochemical and mineral contents of young and mature Polyalthia longifo-lia Sonn. Leaves. Fountain Journal of Natural and Applied Sciences, 3(1):10-19.
65. A.O.A.C.(2000). Association of Official Analyti-cal Chemists. Official Methods of Analysis 19th Edition Washington, D.C Pages 69-77.
66. Duncan, D.B. (1955). Multiple range and multiple F-test. Biometrics 11(1):1-42.
67. Iyayi.E.A. (1994). Supplemental effect of low and high cyanide cassava on the performance, nutrient digestibility and serum metabolites of growing pigs. Journal of Agricultural Tropics and Sub-tropics. 95:199-205.
68. Isaac, L.J., Abah, G., Akpan, B and Ekaette, I.U. (2013). Hematological properties of different breeds and sexes of rabbits (p.24-27). Proceed-ings of the 18th Annual Conference of Animal Science Association of Nigeria.
69. Shittu, M.D., Adesina, G.O and Eseigbe, S. (2019). Productive performance and blood pro-file of weaner rabbits fed different inclusion lev-els of Ipeomoea asarifolia leaf meal in replace-ment of soyabean meal. Journal of Biotechnolo-gy Research, 5(11):107-112.
70. Adeyemo, G.O., Ologbodo, A.D and Adebiyi, O.A. (2010). The effect of graded levels of die-tary methionine on the hematology and serum biochemistry of broilers. International Journal of Poultry Science, 9(2):158-161.
71. Akporhuarho, P.O. (2011). Effect of crude oil polluted water on the hematology of cockerel reared under intensive system. International Journal of Poultry Science, 10(4):287-289.
72. Alikwe,P.C.N., Faremi, A.Y and Egwaikhide, P.A. (2010). Biochemical evaluation of serum metabo-lites enzymes and hematological indices of broiler chicks fed with varying levels of rumen epithelial scraps in place of fish meal proteins. Research Journal of Poultry Science,6(12):855-857.
73. Azeez, O.I., Ayagbemi, A.A and Oyewale,
74. J.O.(2009). Diurnal fluctuation in haematological parameters of the domestic fowl in the hot hu-mid tropics. International Journal of Poultry Sci-ence, 8(3):247-251.
75. Elagib, H.A.A and Ahmed, A.D.A. (2011). Com-parative study on haematological values of blood of indigenous chickens in Sudan. Asian Journal of Poultry Science, 5:41-45.
76. Oloyede, O.B., Minari, J.B and Mohammad, N.O. (2010). Evaluation of growth characteristics and haematological indices of broiler chicks fed raw and processed Bambara nut seed as a compo-nent of poultry feed, 9(7):6 52-655.

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