The plant genome modification by Crispr/Cas9 system is gaining ground. In molecular biology, the use of this indispensable tool works on the basis of target recognition due to the existence of a short protospacer adjacent motif (PAM) that is complementary to the desired strand. The components of the genome-editing tool are guide RNA, Cas9 protein. The mechanistic nature of CRISPR/ Cas9 technology i.e. adaptation, expression and interference lead the desirable mutations in the crop plants. According to the need one can use the different types of programmable DNA targeting and editing platform type. The goal of this review is to provide an idea of the production of stress-tolerant crops. The molecular perception from structural and mechanistic research grant a groundwork for rational engineering for the production of plants that can withstand different kinds of stress.
Alcohol use is part of many cultural, religious and social practices, and provides perceived pleasure to many users. The aim of this project was to develop mini scale alcohol production, which would be very useful for those who focus for many developing countries including Ethiopia, developing this project have been valuable for such sector for those who had an idea to develop new alcoholic beverage project professionals. The paper tried to evaluate the economic performance of minimum level alcohol production, grow to in terms of productivity, profitability, capacity utilization, marketing and the socio-economic contribution of the project. Those, the total capital investments found from this study analysis estimated to be total 34, 673,529.41 birr and the total production cost is 4520833.3birr/yr. The Company will get a net income of 1,376,650,000 birr/year. The repayment for the investment cost will be paid off over the next 4.6 years. The project provides for efficient utilization of the existing resource, technological advancement, managerial efficiency and incentives to upgrade traditional beverages to large production process scales.
Neurofibromatosis type 1 (NF1) is an autosomal predominant, mucocutaneous and inclination disorder. They include it in the development of benign and malignant tumors. Hereditary change in NF1 prompts changes in the outflow of cytoplasmic protein. 90% of all cases represent NF1 in neurofibromatosis. children tainted with NF1, they distinguish them inside a year.NF1 Patients ordinarily experience the ill effects of the café au laitt, freckling, and skeletal dysplasia. The GTPase actuating protein goes about as the principal job in NF1 quality, which fills in as a negative controlled by authoritative with the RAS protein. The neurofibromatosis type 1 quality is a 287-kilo premise of chromosome 17q11.2. In NF1 patients, the dermatologists distinguished the basic cutaneous highlights. Cutaneous neurofibroma has a variable pace of development during life expectancy. The threatening fringe nerve sheath tumor is plexiform neurofibromas. Palpebral plexiform neurofibroma is mono-sidelong. We have analyzed inherent dysplasia real criteria for NF1. The more exact technique to analyze NF1 in the patient is radiological indicative. An entire body MRI can test the interior nerve sheath tumor. They have announced that the research center analytic is extremely troublesome; However , it has done the RNA and ELISA tests somewhat. The treatment of NF1 patients is exceptionally troublesome because of the adequacy of the organ of the body. There is a biopsy, medical procedure, and radiotherapy performed for NF1 patients.
Current practices in Greek broiler farms as related to the technical status of the establishments and equipment
The aim of this study was to describe breeding and managerial practices performed in two main categories of broiler farms as related to their technical status i.e. years of functioning, facilities, equipment, etc. One hundred thirty two farms, randomly selected, sited in regions which represent more than 70% of the Greek broiler production were examined. A structured questionnaire was used to collect data by personal interviews with the farm managers including biosecurity measures, harvesting system, feeding and watering method, staff, microclimate in broiler house, antimicrobial therapy and information about the establishment and equipment of the farm as well as the genotype, age and population of the flock. First results indicate that biosecurity measures in farms in good technical status perceive more importance (presence of anteroom and foot bath, P≤0.05) than farms in bad status. Moreover, 93.3% of the farms in bad technical status use straw as bedding material in contrast with the 28.2 % of the farms in good status where rice hulls is used (P≤0.05). Both categories of farms rear Ross genotype while Cobb genotype is only reared in farms in good technical status (P≤0.05). Finally, water equipment differs significantly (P≤0.05) between the two farm categories. Nipple drinkers without a tray is used by 40.4% of the farms in good technical status while 58.3% the farms in low level of hygiene have water equipment with nipple drinkers with tray. This study presents preliminary results of a PhD thesis in progress aiming to investigate the prevalence of resistant strains of Escherichia Coli producing ß-lactamases in poultry farms.
Objective: To establish the effects of long-term use and preventive value of metformin in geriatric women with PCOS. Methods: A search of published review articles was carried out in sites such as NCBI, ScienceDirect, Elsevier, Springer, Wolters Kluwer, among others, using the key words: “polycystic ovary syndrome”, “PCOS”, “Polycystic ovary syndrome and metformin”, “effects of metformin and PCOS“,” insulin resistance and metformin “and” long-term sequelae of polycystic ovarian syndrome“. Results: We retrospectively analyzed data specific to the randomized clinical trial of Pedersen et al. 2017, to determine the impact that treatment had for twelve months with metformin in three specific variables: weight, total cholesterol and HDL. The study was conducted in 40 Caucasian women from 18 to 39 years of age, with a fixed dose of metformin (2 g / day for 12 months), who met the Rotterdam criteria for PCOS in their genetic variant MATE1 with SOP7. Conclusions: It was confirmed that the establishment of twelve-month treatment with metformin in the randomized clinical trial of Pedersen et al. 2017 had a significant effect on the three specific variables: weight, total cholesterol and HDL.
A new proposed translation of the “James ossuary” inscription: Jaqob, son of Jeshua’s brother Joseph or Jaqob, son of Joseph, nephew of Jeshua
In the first part of this paper I recall the long and dramatic story of ossuary owner Mr Oded Golan. In 2003 Mr Golan was accused by the Israel Antiquities Authority (IAA) of the crime of forgery of the second part of the Aramaic inscription on the long side of the ossuary, יעקובבריוסףאחוידישוע , translated by Prof. André Lemaire “James son of Joseph brother of Jeshua”. Between 2007 and 2012, a very detailed case conducted at the New Court in Jerusalem concluded that Mr Oded Golan was NOT GUILTY of forgery. During this case the authenticity of the entire Aramaic inscription was supported by new scientific evidence, including the presence of a biopatina (created by microorganisms living for centuries on the lime box) covering all walls and letters of the inscription. In the second part of this paper I propose a new translation of the inscription. The word “achui d” may have been mistranslated because brother translates to “ach” in Aramaic. Prof. Fitzmyer showed that these same words appear in a verse in the Dead Sea Scrolls found in cave 1, the Genesis Apocryphon (21:34–22:01) (QapGen, written in the 1st century BC). The verse mentions “Lot, son of Abram’s brother”, which corresponds to Genesis 14:12. According to Genesis 11:27, Terah had three sons: Abram, Nahor, and Haran. Haran was Lot’s father, and therefore Lot was Abram’s nephew, i.e. the son of Abram’s brother. This means that “achui d” is the word for “brother” in a genitive case that in English should be translated by name conversion as Lot, son of Abram’s brother Haran. It then follows that the inscription should be translated as Jaqob, son of Jeshua’s brother Joseph. This means that Jeshua and Joseph were brothers, and Jaqob was Jeshua’s nephew.
Neonate with 10q Interstitial Deletion within the Long Arm of Chromosome 10- A Case Report and Literature Review
Introduction: Partial deletion of distal chromosome 10q was first reported in 1978 by Lewandowski1. Interstitial deletions within bands 10q25e10q26.3 are rare. Seven such cases were reported so far2. Patient Information: A term AGA male newborn was delivered at our perinatal center with antenatal diagnosis of unbalanced translocation of chromosomes 10 and 12, and fetal cleft lip and cleft palate. Blood was sent for chromosome analysis using GTG banding method. Baby had facial dysmorphism, left cleft lip, bilateral cleft of soft and hard palate, intact nasal septum, normal ears and micrognathus. Abdominal ultrasound showed absence of right testis in inguinal canal and abdomen (anorchia). Hospital course was unremarkable except for feeding problems requiring feeding team, plastic surgery planned at 2- 3 months of age, and taping of the cleft lip. He went home on day 7. Conclusion: Here, we reported an extremely rare case of a male newborn with an interstitial deletion within the long arm of chromosome 10 between bands 10q25.1 and 10q26.1, with dysmorphic features, along with a few unreported associations (atypical Pierre-Robin sequence and bilateral dorsal horn ventriculomegaly). We added a comprehensive review of literature on chromosome 10q deletions to the case report. We also listed clinical implications of 71 RefSeq and OMIM genes noted in that ~13.3Mb 10q deletion in the Appendix. Earlier detection of both common and rare chromosomal- genetic abnormalities might prepare the family and health care team to plan optimal care to the mother, and baby.
Main purpose: This paper aimed to confirm a relationship between chronic hypertrophic tonsillitis (CHT) and bad mouth odour. To this end, detailed identification of the microbial flora inhabiting affected tonsils was carried out. The results obtained might be helpful in specifying indications for tonsillectomy. Materials and method: From among 247 patients with clinically diagnosed CHT, 33 generally healthy individuals aged 18- 40 (10 male and 23 female) were selected. Patients in whom other causes could be the possible reason for their fetor ex ore (halitosis) were not included. Before and 2 to 3 months after tonsillectomy, organoleptic and halimeter testing was undertaken for each patient. A swab was collected from the interior of the enucleated tonsils in a sterile manner, and was inoculated onto surfaces enabling the culture of aerobic and anaerobic bacteria and fungi. A histopathological examination was subsequently performed. Results: Fetor ex ore was initially found in 95% of the patients with CHT. In 90% of cases with confirmed halitosis, after tonsillectomy a significant reduction in its intensity was observed. On average, the concentration of VSC in the patient decreased by approximately 75 ppb (62%), which was statistically significant (p < 0.0001). It was also shown that the concentration of VSC in carriers of anaerobic bacteria, compared to carriers of only aerobic bacteria, was significantly reduced (p < 0.05). The results obtained confirm the role of CHT in the pathogenesis of halitosis. Conclusions: Halitosis in patients with CHT requires a number of laboratory tests and specialist consultations to exclude other possible causes of fetor ex ore. A dental examination plays an important role in the differential diagnosis. Performing targeted microbiological testing to determine the patient’s carrier state for anaerobes should eventually be considered. When these conditions are met, halitosis can be considered an independent indication for tonsillectomy.
Background: Health workers get sick annually because of the characteristics and routine of their work and the peculiarity of dealing with the pain of others. Being sensitive and predisposed to care for the other suffering, involves empathy and compassion. Continuous and prolonged exposure to this labor context can generate stress and exhaustion of the professional, due to prolonged affectation with the pain and suffering of others, which can cause fatigue through compassion. Objective: to reflect about compassion fatigue in the context of health. Methodology: bibliographic review. Results and discussion: Health professionals present a commitment that moves them to relieve and intervene in the pain of the other. When the care is lived exhaustively and chronically, with the excessive use of compassion, it can directly affect the health of the worker, interfering in their quality of life and in the execution of their work, leading to fatigue by compassion. Final considerations: Fatigue of compassion can impact psychologically on health professionals and the ability to tolerate pain and suffering of the patient, interfering negatively in their work. Therefore, it is necessary that more research be developed in the different fields of professional work in health, aiming to stimulate reflections and actions focused on the quality of life of these professionals
Introduction: Communication is a central element in any social situation, be it in the ordinary context of everyday life, or in a moment of illness. When health is compromised, communication should be exemplary between the health professional and the patient, but this is not always what happens. Several aspects emerge in this context, with a sender sending the message to a receiver, which may suffer contextual, cultural and individual influences. Every communication process is susceptible to interference, which is called noise, which can compromise the receiver’s understanding of the message, causing distorted interpretations and inappropriate reactions, which may occur due to the situational and subjective aspects of those involved. Objective: To report situations in the hospital psychological practice that identified interference in communication and the psychologist acted as a mediator, aiming to minimize the suffering of the patient. Methodology: Experience report. Results and Discussion: When communication takes place in a health context, it becomes more delicate and careful, as it involves a condition sometimes of suffering, pain, impaired perceptual abilities, regressive state of the patient, denial of the condition. illness, or pharmacological therapy. In this scenario, these elements compromise the understanding of information and guidance provided for recovery. Depending on what the patient experiences in the hospital environment, they can signal the most diverse messages, ranging from signaling life expectancy to the prospect of death. Cultural, social, emotional and psychological aspects are present, as well as physiological evidence. Common situations of interference in communication, which generated noise and a misunderstanding about the diagnosis, procedures, use of medications, postoperative were: false compliments about the patient’s condition, reinforcement of denial of reality, explanations of partial and fragmented procedures, of treatment steps with excessive use of technical terms, double message about evolution, rough touch, facial expressions and gestures that point to an inconsistency…