Global Journal of Molecular Biology

Identification and susceptibility of the genus staphylococcus isolated from vegetables and legumes of economic interest

Research Article of Global Journal of Molecular Biology Identification and susceptibility of the genus staphylococcus isolated from vegetables and legumes of economic interest Amaral,J. P. D. l1; Ferreira Neto, J. P2; Magalhães, L. P. M. 3; Lima, C. S. A4; Yara, R. 4; Sena, K. X.F. R. 5 1Student of the Biomedicine Course, UFPE; 2 Student of the Morfotecnology postgraduate program, UFPE; 3Student of the postgraduate program in Pharmaceutical Sciences, UFPE; 4Teacher/Researcher of the Department of Biophysics, UFPE; 5Teacher/Researcher of the Department of Antibiotics, UFPE. Introduction: Microorganisms of Staphylococcus genus are Gram-positive cocci catalase-positive bacteria of clinical interest due to their pathogenicity in humans and animals. Staphylococcus aureus is part of the normal microbiota and can be responsible for suppurations of wounds, abscesses also it may transmit a toxinfection caused by the intake of toxins elaborated by the infectious agent present in foods. Objectives: Isolation and identification of Staphylococcus in products of economic interest cultivated in Pernambuco. Material and Methods: 25 g of the samples (cassava, carrot, coriander and cabbage) were added in 225 mL of 0.1% peptone saline, serial dilutions until 10-3 were made. The dilutions of 10-2 and 10-3 were seeded in Baird Parker medium and the colonies characteristic of the genus Staphylococcus were identified by the catalase, Gram, coagulase, Carbohydrate (Glucose, Trehalose, Ramnose, Mannose, Maltose, Lactose, Xylose, Sucrose and Inositol) and disc test with Polymyxin and Novobiocin. In addition, the Antibiogram of the isolated colonies was performed using the antibiotics Erythromycin, Vancomycin, Clindamycin, Sulfamethoxazole, Oxacillin, Chloramphenicol, Gentamicin and Ciprofloxacin. Results and Discussion: Eight colonies were isolated: cassava (1), coriander (1), carrot (1) and cabbage (5) and submitted to identification tests. Staphylococcus pasteuri, S. saprophyticus, S. warneri (3), S. hominis subsp. hominis and two classified as coagulase negative Staphylococcus. In the antibiogram 50% of the isolates presented resistance ...

Modulation of plant extracts on the resistance of staphylococcus aureus strains to antibiotics

Research Article of Global Journal of Molecular Biology Modulation of plant extracts on the resistance of staphylococcus aureus strains to antibiotics Amaral, J. P. D.1; M agalhães,L. P. M. 2; Oliveira, C. V. J. 3; Lima, C. S.A. 4; R Yara, R. 4; Sena, K. X. F. R 5 1Student of the Biomedicine Course, UFPE; 2Student of the postgraduate program in Pharmaceutical Sciences, UFPE; 3 Student of the postgraduate program in Biological Sciences, UFPE; 4Teacher/Researcher of the Department of Biophysics, UFPE. 5Teacher/Researcher of the Department of Antibiotics, UFPE Introduction:The increase in bacterial resistance has generated the need to increase research for the discovery of new drugs, and medicinal plants are a proven source of bioactive products with great therapeutic potential. Objectives: To analyze the modulating activity of the ethanolic extracts of cumaru, angico, artemisia, terramicina and espinheira in front of strains of Staphylococcus aureus. Methods: Modulating activity was determined by the disc diffusion method, analyzing three strains of multiresistant Staphylococcus aureus with the antibiotics erythromycin, vancomycin, clindamycin, oxacillin, gentamicin, chloramphenicol, cipropofloxacin and cefoxitin. Each antibiotic disk was soaked with 20μL of the extract. The disks were placed on the surface of the culture medium already seeded with the microorganisms and incubated at 35 ° C for 24 hours. The diameters of inhibition halos (HI) were measured in mm and compared to those determined by HI of the antibiotics alone. The increase in HI diameter ≥ 2 mm, synergistic effect; antagonistic effect when the HI diameter was smaller than that of the isolated antibiotic; and, indifferent effect, when the increase in HI diameter <2 mm in relation to the antibiotic alone. Results and Discussion: The crude extracts evaluated were able to modulate the activity of different antibiotics of clinical use by means of synergistic, indifferent or antagonistic actions. For the UFPEDA ...

Use of Glutamine as Supplementation in Oncological Patients in Treatment That Present Mucosite

Review Article of Global Journal of Molecular Biology Use of Glutamine as Supplementation in Oncological Patients in Treatment That Present Mucosite Pinheiro, M. M.A 1, Silva, K. B. 2; Vila Nova Filho, S. L 3, Nascimento, G. S 4, Santos, J. P. O. 5 1Nutricionista graduada pela Unifavip DeVry, 2 Nutricionista Residente em Atenção ao Câncer e Cuidados Paliativos – ASCES UNITA, 3Nutricionista Mestrando no Programa de Pós-graduação em Saúde as Criança e do Adolescente do Centro de Ciências da Saúde- UFPE, 4 Enfermeira Residente em Atenção ao Câncer e Cuidados Paliativos, 5 Assistente Social Residente em Atenção ao Câncer e Cuidados Paliativos. Introduction:The treatment used in neoplasias, chemotherapy and radiotherapy, generate a series of side effects that undermine the prognosis of cancer patients. Aiming mainly at nutritional status and mucosal integrity, we found the need for prevention in cases of patients who present with mucositis during anti-neoplastic treatment. Objective: To carry out a systematic review of the available knowledge about glutamine supplementation in the treatment of mucositis in patients undergoing antineoplastic treatment. Method: This is a literature review, selecting articles published between 2013 and 2015, in the Medline and Lilacs databases through Pubmed, with the following descriptors: glutamine and radiotherapy, glutamine and cancer and mucositis. Results: Studies indicate that the tumors that most affect the nutritional status of patients are head and neck neoplasms, esophagus, digestive tract and lung. Considering that the side effects of antineoplastic agents, such as vomiting, nausea, diarrhea, xerostomia, increased basal metabolic rate and mucositis, trigger a decrease in food intake, Leading the patient to malnutrition and cachexia. Mucositis is a result of inflammation of the oral mucosa or gastrointestinal tract. Several studies have evaluated the use of glutamine supplementation during antineoplastic treatment, since it depletes it over time, being related to cachexia, loss of ...

Dr. Hala Abdel Wahab
Ph.D., Université de Paris-Sud, France, Associate Professor, Department of Plant Pathology Head of Lab. “Molecular Diagnostic of Plant Diseases” Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Dr. Shahin Gavanji
Chairman of “WAMS National Council of Iran at World Academy of Medical Sciences (WAMS), Chairman WAMS Oncology Research Group of Iran, 3-Young Researchers and Elite Club, Khorasgan Branch, Islamic Azad University, Khorasgan, Isfahan, Iran, Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan

Dr. Richa Madan Lomash
Research Fellow, Receptor Biology Unit (NINDS), NIH, Bethesda (MD), USA

Dr. Alessandra Pelagalli
School of Veterinary Medicine, University of Naples

Professor, Department Of Chemistry, Sri Vidya Nikethan Engineering College.Tirupathi ,INDIA

Dr Jahwarhar Izuan Bin Abdul Rashid
Department of Chemistry & Biology, Centre for Defence Foundation Studies (CDFS), Defence National University of Malaysia

Dr. Omi Laila Majeed
Division of Basic Sciences and Humanities, Sher-e-Kashmir University of Agricultural Sciences and Technology

Dr. Mohammad Mehdizadeh
PhD of Weed Science, University of Mohaghegh Ardabili

Dr Amanjot Kaur Riar
Brigham and Women’s Hospital- Harvard Medical School, Department of Medicine, Harvard New Research Building USA

Manuscript Title: The title should be a brief phrase.

Author Information: List full names and affiliation of all authors, including Emails and phone numbers of corresponding author.

Abstract: The abstract should be less than 500 words. Following abstract, a list of keywords and abbreviations should be added. The keywords should be no more than 10. Abbreviation are only used for non standard and long terms.

Introduction: The introduction should included a clear statement of current problems.

Materials and Methods: This section should be clearly described.

Results and discussion: Authors may put results and discussion into a single section or show them separately.

Acknowledgement: This section includes a brief acknowledgment of people, grant details, funds

References: References should be listed in a numbered citation order at the end of the manuscript. DOIs and links to referenced articles should be added if available. Abstracts and talks for conferences or papers not yet accepted should not be cited. Examples Published Papers: 

1.Teixeira C.L; Picanço K.R; Silva F.J; Alves A.J; Souza Neto Júnior J.C.Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX.Global Journal of Molecular Biology, 2018, 1:5. DOI: 10.28933/gjmb-2018-05-1805 
2.Melo T.S; Oliveira C.V.J; Amaral J.P.D; Lima G.M.S; Sena K.X.F.R. To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes.Global Journal of Molecular Biology, 2018, 1:4. DOI: 10.28933/gjmb-2018-05-1804 

Tables and figures: Tables should be used at a minimum with a short descriptive title. The preferred file formats for Figures/Graphics are GIF, TIFF, JPEG or PowerPoint.

Publication fee: The authors will be contacted about the publication fee after a manuscript have been accepted.

Proofreading and Publication: A proof will be sent to the corresponding author before publication. Authors should carefully read the proof to avoid any errors and return the proof to the editorial office. Editorial office will publish the article shortly and send a notice to authors with the links of the paper.

Open Access

Global Journal of Molecular Biology is a peer reviewed open access journal publishing research manuscripts, review articles, editorials, letters to the editor in Molecular Biology (Indexing details).

Peer Review

To ensure the quality of the publications, all submitted manuscripts will be peer-reviewed by invited experts in the field. The decisions of editors will be made based on the comments of the reviewers.

Rapid Publication

Time to first decision: within 2 days for initial decision without review, 18 days with review; Time to publication: Accepted articles will be published online within 2 days, and final corrected versions by authors will be accessible within 5 days.  More details....

Rapid Response Team

Please feel free to contact our rapid response team if you have any questions. Our customer representative will answer you shortly.

Global Journal of Molecular Biology