Global Journal of Molecular Biology


Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX

Research Article of Global Journal of Molecular Biology Hematological parameters and sorological detection of canine hemoparasitoses with SNAP 4DX Teixeira C.L1; Picanço K.R2; Silva F.J3; Alves A.J4; Souza Neto Júnior J.C5 1Zootechnist and Specialization in Clinical and Toxicology Analysis Student; 2Clinical and Toxicology Analysis Specialist; 3Specialization in Clinical and Toxicology Analysis Student - UFPE; 4Full Professor – UFPE; 5Doctor of Veterinary Medicine - UFRN Introduction: The SNAP 4Dx is a rapid test device that uses ELISA (Enzyme-Linked Immunosorbent Assay) technology manufactured by the IDEXX Laboratory (Maine, USA) for rapid diagnosis of hemoparasitosis in dogs. SNAP® ELISA technology utilizes unique conjugate and substrate that amplifies the results. The bidirectional flow process evidences the antigen-antibody binding, providing high sensitivity and reliable results. Objective: evaluation of hematological parameters and serological detection of hemoparasitoses in dogs with Snap 4Dx. Methodology: A cross - sectional, descriptive and observational clinical research was carried out between September 2014 and October 2014 at Pet Dream Veterinary Medical Clinic, located in the city of Recife / PE. The hemogram was performed using a manual technique using blood collected with EDTA. The detection of the antigen of Dirofilaria immitis and the antibodies of Anaplasma phagocytophilum, Anaplasma platys, Borrelia burgdorferi, Ehrlichia canis and Ehrlichia ewingii, was performed with SNAP 4DX Plus device. Results and Discussion: 62 dogs of different races were used, without specifying age or sex, attended at the Clinic. The blood samples were collected by venipuncture cephalic or jugular, according to the size of the animal. The samples were packed in eppendorf microtubes containing EDTA as anticoagulant. The differential count was performed through blood smearing, stained in panopticus, observed with a 100X immersion objective. The rapid test for serological diagnosis showed the following result: Ehrlichia canis (32.25%), Anaplasma platys (6.45%), Dirofilaria immitis (6.45%). Discussion: The results found in the ...

To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes

Research Article of Global Journal of Molecular Biology To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes Melo T.S1; Oliveira C.V.J2; Amaral J.P.D3; Lima G.M.S4; Sena K.X.F.R4 1Student of the Pharmacy Course, UFPE; 2Student of the Postgraduate Program in Biological Sciences, UFPE; 3Student of the Biomedicine Course, UFPE; 4Teacher/Researcher of the Department of Antibiotics, UFPE. Introduction: Streptomyces comprise the genus of actinobacteria most studied, as they have the ability to synthesize a variety of bioactive metabolites, of these many are antibiotics. Streptococcus pyogenes is a gram-positive coccus belonging to Group A of Lancefield. Among streptococci, it is the most relevant clinical agent in infections of the oral cavity due cases of streptococcal pharyngotonsillitis and its sequels: rheumatic fever and glomerulonephritis. They are also related to other pathologies such as endocarditis, septic arthritis, cellulitis, pyoderma and scarlatina. Objective: To evaluate the antimicrobial potential of Streptomyces spp. J181 against clinical isolates of S. pyogenes. Methodology: Blocks of 10 mm diameter obtained from the cultivation of the actinobacteria seeded on ISP-2 agar were transferred to Petri dishes containing 18 mL of blood agar previously seeded with S. pyogenes, then the plates were incubated at 35 ° C ± 1 ° C for 24 hours. After the incubation period, the inhibition halos were read in millimeters. To assess the susceptibility profile, the antibiogram of the bacterial isolates was carried out according to the recommendations of the Clinical & Laboratory Standards Institute. The tests were performed in triplicate, the results expressed in average plus standard deviation and the coefficient of variation adopted was ≤ 10%. Results and Discussion: The susceptibility profile of S. pyogenes isolates didn’t show resistance to the evaluated antibiotics, resulting in sensitivity to therapy commonly adopted in the medical clinic. The lineage of Streptomyces spp. J181 ...

Identification and susceptibility of the genus staphylococcus isolated from vegetables and legumes of economic interest

Research Article of Global Journal of Molecular Biology Identification and susceptibility of the genus staphylococcus isolated from vegetables and legumes of economic interest Amaral,J. P. D. l1; Ferreira Neto, J. P2; Magalhães, L. P. M. 3; Lima, C. S. A4; Yara, R. 4; Sena, K. X.F. R. 5 1Student of the Biomedicine Course, UFPE; 2 Student of the Morfotecnology postgraduate program, UFPE; 3Student of the postgraduate program in Pharmaceutical Sciences, UFPE; 4Teacher/Researcher of the Department of Biophysics, UFPE; 5Teacher/Researcher of the Department of Antibiotics, UFPE. Introduction: Microorganisms of Staphylococcus genus are Gram-positive cocci catalase-positive bacteria of clinical interest due to their pathogenicity in humans and animals. Staphylococcus aureus is part of the normal microbiota and can be responsible for suppurations of wounds, abscesses also it may transmit a toxinfection caused by the intake of toxins elaborated by the infectious agent present in foods. Objectives: Isolation and identification of Staphylococcus in products of economic interest cultivated in Pernambuco. Material and Methods: 25 g of the samples (cassava, carrot, coriander and cabbage) were added in 225 mL of 0.1% peptone saline, serial dilutions until 10-3 were made. The dilutions of 10-2 and 10-3 were seeded in Baird Parker medium and the colonies characteristic of the genus Staphylococcus were identified by the catalase, Gram, coagulase, Carbohydrate (Glucose, Trehalose, Ramnose, Mannose, Maltose, Lactose, Xylose, Sucrose and Inositol) and disc test with Polymyxin and Novobiocin. In addition, the Antibiogram of the isolated colonies was performed using the antibiotics Erythromycin, Vancomycin, Clindamycin, Sulfamethoxazole, Oxacillin, Chloramphenicol, Gentamicin and Ciprofloxacin. Results and Discussion: Eight colonies were isolated: cassava (1), coriander (1), carrot (1) and cabbage (5) and submitted to identification tests. Staphylococcus pasteuri, S. saprophyticus, S. warneri (3), S. hominis subsp. hominis and two classified as coagulase negative Staphylococcus. In the antibiogram 50% of the isolates presented resistance ...

Modulation of plant extracts on the resistance of staphylococcus aureus strains to antibiotics

Research Article of Global Journal of Molecular Biology Modulation of plant extracts on the resistance of staphylococcus aureus strains to antibiotics Amaral, J. P. D.1; M agalhães,L. P. M. 2; Oliveira, C. V. J. 3; Lima, C. S.A. 4; R Yara, R. 4; Sena, K. X. F. R 5 1Student of the Biomedicine Course, UFPE; 2Student of the postgraduate program in Pharmaceutical Sciences, UFPE; 3 Student of the postgraduate program in Biological Sciences, UFPE; 4Teacher/Researcher of the Department of Biophysics, UFPE. 5Teacher/Researcher of the Department of Antibiotics, UFPE Introduction:The increase in bacterial resistance has generated the need to increase research for the discovery of new drugs, and medicinal plants are a proven source of bioactive products with great therapeutic potential. Objectives: To analyze the modulating activity of the ethanolic extracts of cumaru, angico, artemisia, terramicina and espinheira in front of strains of Staphylococcus aureus. Methods: Modulating activity was determined by the disc diffusion method, analyzing three strains of multiresistant Staphylococcus aureus with the antibiotics erythromycin, vancomycin, clindamycin, oxacillin, gentamicin, chloramphenicol, cipropofloxacin and cefoxitin. Each antibiotic disk was soaked with 20μL of the extract. The disks were placed on the surface of the culture medium already seeded with the microorganisms and incubated at 35 ° C for 24 hours. The diameters of inhibition halos (HI) were measured in mm and compared to those determined by HI of the antibiotics alone. The increase in HI diameter ≥ 2 mm, synergistic effect; antagonistic effect when the HI diameter was smaller than that of the isolated antibiotic; and, indifferent effect, when the increase in HI diameter <2 mm in relation to the antibiotic alone. Results and Discussion: The crude extracts evaluated were able to modulate the activity of different antibiotics of clinical use by means of synergistic, indifferent or antagonistic actions. For the UFPEDA ...

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Global Journal of Molecular Biology

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