Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia

Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts, Benishangul Gumuz Regional State, Ethiopia

Yami Bote, Kibeb Legesse, Asmelash Tassew

Addis Ababa University, College of Veterinary Medicine and Agriculture P.O.Box: 34; Debre Zeit, Ethiopia

International Journal of Animal ResearchA cross sectional study was conducted from November 2016 to May 2017 in Bambasi and Assosa districts in Benshangul Guzum Regional State with the objectives of isolation, identification and determination antimicrobial susceptibility profile of Pasteurella multocida isolated from cattle infected with Hemorrhagic Septicemia. The study was also aimed to detect the agent using classical Polymerase chain reaction. Specimens were collected purposively from cattle in areas with outbreak of Hemorrhagic septicemia and a total of 384 swab samples were collected aseptically from cattle and transported to Assosa Regional Veterinary Laboratory using a cold chain system. Specimens were cultured on MacConkey agar and blood agar. Different biochemical tests were also used in order to identify and characterize the agent of the suspected disease. The prevalence of P. multocida was 13 (3.39%) out of 384 samples tested). All the isolates were subjected to the in vitro antimicrobial ssensitivity tests and the result indicated the presence of different degrees of susceptibility and resistance to most of the antibacterial agents. The degree of susceptibility ranges from 15.4% for Tetracycline upto 61.5% for Sterptomycin, and resistance is between 15.4% for Sterptomycin, Clindamycin and Chloramphicol and 69.2% for Tetracycline. Similarly, (60 %) of this discs showed the presence of multidrug resistance. The result of this test clearly indicates that, Sterptomycin, Clindamycin and Chloramphicol could be prospective drugs of choice. Analysis of PCR assay revealed the presence of P. multocida serotype B2. In conclusion, the presence of P. multocida serotype B2 in the selected areas can be considered as potential pathogens in causing Hemorrhagic septicemia in cattle especially where there is no any practice of vaccination. Therefore, strict measures like proper vaccination and antibiogram test to select effective drugs should be regularly implemented.

Keywords: Antimicrobial sensitivity test, Benishangul Gumuz Regional State, Cattle Haemorrhagic Septicemia, Isolation, Pasteurella multocida, Polymerase chain reaction

Free Full-text PDF

How to cite this article:
Yami Bote et al., Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts, Benishangul Gumuz Regional State, Ethiopia. International Journal of Animal Research, 2017; 1:4. DOI:10.28933/ijar-2017-09-2301


1. Ashraf A, Tariq H, Shah MS, Nadeem S, Manzoor I, Ali S, Ijaz A, Gailani S, Mehboob S. (2011): Characterization of Pasteurella multocida strains isolated from cattle and buffaloes in Karachi, Pakistan. African Journals Microbiology Research; 5(26):4673-4677.
2. Assosa district office of livestock and fishery development,(2013)
3. Bamibasi district office of livestock and fishery development,(2015).
4. Blackall, P., Bojesen, A., Christensen, H. and Bisgaard, M. (2007): Reclassification of [Pasteurella] trehalosi as Bibersteinia trehalosi. Systematic and Evolutionary Micr- obiology, 57, 666−674.
5. Biswas., Shivachndra S.B., Saxena M.K., Kumar A.A., Singh V.P. & Srivastava S.K. (2004): Molecular variability among strains of P. multocida isolated from an outbreak of haemorrhagic septicaemia in India. Veterinary Research and Commu- nication, 28, 287–298.
6. Catry, B. (2005): Pasteurella and Mannheimia species from calves: differentiation and antimicrobial resistance, PhD thesis, Ghent University, Merelbeke, Belgium.
7. Cheesbrough M (2006): District Laboratory Practice in Tropical Countries.Part 2.2nd edition, Cambridge University Press, New York. USA. pp. 45-62.
8. Choudhury K.A., Amin M.M., Rahman A., Ali M.R. (1985): Investigation of natural outbreak of fowl cholera. Bangladesh Vetterinary Journal 19: 49-56.
9. Clinical and laboratory standard institute (CLSI), (2012): Performance standared for antimicrobial susceptibility testing; Tw. Sec. info. Sup. 32:100-522.
10. De Alwis, M. C. L. (1999): Hemorrhagic septicemia. Canberra: Austeralian Center for International Agricaltural.Research. pp. 1–10.
11. De Alwis M.C.L. and Sumanadasa M.A. (1982): Naturally acquired immunity to haemorrhagic septicaemia among cattle and buffaloes in Sri Lanka. Tropical Animal Health and Production, 14, 27-28.
12. De Alwis, M.C.L., Kodituwakku, A.O. and Kodituwakku S. (1976): Hemorrhagic septicemia an analysis of two outbreaks of disease among buffaloes Ceylon veterinary Journals, 24, 18-21.
13. Dereje, A., Tesfaye, S., Tadesse, B. (2013): Isolation and Identification of Mannhemia and Pasteurela species from pneumonic and apparently health cattle and their antibiogram susceptibility pattern in Bedelle district, Western Ethiopia. Journal of Bacteriology Res- earch,Vol. 6 (5), p. 32-41.
14. Dowling, A., J.C. Hodgson, M.P. Dagleish and P.D. Eckersall, (2004): Pathology, Veterinary Immunology, Sale Journal, 100: 197-207.
15. Environmental Agency (2002): The Microbiology of Drinking Water, (2002). Methods for Examination Water and Associated Materials .p.10-14.
16. Ethiopia animal health yearbook, (2011): Summary of disease outbreak reports during 2009-2010 Fiscal Year, PP.41.
17. Garner, G., Saville ,P., Fediaevsky, A. (2003): Manual for the recognition of exotic diseasesof livestock: A reference guide for animal health staff [online] Food and Agriculture Organization of the United Nations [FAO]; Hemorrhagic septicemia.
18. Hawari, A.D., D.S. Hassawi and M. Sweiss (2008): Isolation and Identification of Mannheimia haemolytica and pasteurella multocida in Sheep and Goats using Biochemical Tests and Random Amplified Polymorphic DNA (RAPD) Analysis Journals Biological Science. 8 (7):1251-1254.
19. Heath protection agency (2010): Indole test. National Standard Method BSOP TP19 issue2
20. Katherine, O. (2008): Pasteurella multocida. Pasteurellosis, 7, 26-45.
21. Kehrenberg C., Schulze-Tanzil G., Martel J.-L., Dancla E.C. & Schwarz S. (2001): Antimi- crobial resistance in Pasteurella and Mannheimia: epidemiology and genetic basis. Veterinay Research, 32, 323–339.
22. Kumar, A. A.; Shivachandra, S. B.; Biswas, A.V.; Singh, P.; Singh Vijendra, P., & Srivastava, S. K. (2004): Prevalent serotypes of Pasteurella multocida isolated from different animal and avian species in India. Canadian Journal Veterinary Research Community, 28: 657-667.
23. Michael, W. (2008): Pasteurellosis Transmission Risks between Domestic and Wild Sheep. CAST Commentary. Pp 325.
24. Mulaw, S., Addis, M. and Fromsa, A.(2011): Study on the Prevalence of Major Trypanosomes Affecting Bovine in Tsetse Infested Asosa District of Benishangul Gumuz Regional State, Western Ethiopia. Global Veterinaria .,4:Pp 330-336.
25. National Meterology Service Agency (NMSA, 2007).
26. Ragy, S. (2005): Major Pathogenic Components of Pasteurella multocida and Mannheimia (Pasteurella) haemolytica Isolated From Animal Origin. Veterinary Microbiology, 17, 312-356.
27. Rahman M.H., Shahiduzzaman A.N.M, Haque M.E., Nazir K.H.M.N.H., Rahman (2016): Development of Experimental Oil based Inactivated HS Vaccine from field Isolates of Pasteurella multocida from Cattle in Bangladesh. Inerination Journal Vaccines and Vaccination 2(2) 15406.
28. Refsdal, A.O. (2000): To treat or not to treat:a proper use of hormones and antibiotics. Animals Reproduction Science, 60- 61: 109-119
29. Richard, W. (2009): Outer Membrane Proteomics of Pasteurella multocida Isolates to Identify Putative Host-Specificity Determinants. Bioscience Horizons, 2, Issue1, pp1-12
30. Sheikh M.A., Anzam, M. and Shakoori, A.R. (1996): Observations on haemorrhagic septicaemia in Pakistan livestock. Journal of Veterinary Medicine, Series B, 43, 293-304. Abstract.
31. Shivachandra S.B., Viswas K.N., Kumar A.A. (2011): A review of hemorrhagic septicemia in cattle and buffalo. Animal Health Research Review 12: 67-82.
32. Shivachandra S.B, Kumar A.A., Gautam R., Singh V.P., Chaudhuri P., and Srivastava S.K., (2004a). PCR assay for rapid detection of Pasteurella multocida serogroup-A in morbid tissue materials from experimentally induced Fowl cholera in chicks. Veterinary Journal 168: 349–352.
33. Townsend, K. J., Boyce, J., Chung, A., Frost and Adler, B. (2001): Genetic organization of Pasturella multocida cap loci and development of multiplex capsular PCR typing system. Jouinal Clinical and Microbiology, 39: 924-929.Pasteurella multocida. Journal of Clinical Microbiology, 36, 1096-1100.
34. Townsend K.M, Frost A.J., Lee C.W., Papadimitriou J.M. and Dawkins H.J.S. (1998): Development of PCR assays for species and type specific identification.
35. Zuber, I. (2009): Epidemiological Study of Pasteurellosis in Lambs in Erbil Governorate, Northern Iraq. UDC, 619, 579.84.

Terms of Use/Privacy Policy/ Disclaimer/ Other Policies:
You agree that by using our site, you have read, understood, and agreed to be bound by all of our terms of use/privacy policy/ disclaimer/ other policies (click here for details).

This work and its PDF file(s) are licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.