Effects of Dried Centella Asiatica Leaf Meal as a Herbal Feed Additive on the Growth Performance, Heamatology And Serum Biochemistry of Broiler Chicken

Effects of Dried Centella Asiatica Leaf Meal as a Herbal Feed Additive on the Growth Performance, Heamatology And Serum Biochemistry of Broiler Chicken

Alagbe, J.O

University of Abuja, Gwagwalada, Nigeria.

International Journal of Animal Research

A 42 days experiment was carried out to evaluate the effects of dried Centella asiatica leaf meal (CSP) as an herbal feed additive on the growth performance, haematology and serum biochemistry of broiler chicken. A total of 200 Ross 308 day old broiler chicks of mixed sex was divided into four (4) treatment groups of fifty (50) birds, each group was further divided into five replicates each of ten (10) birds. Group A was fed basal diet + 0% CPS (control), group B,C and D were fed basal diet + 2%, 4% and 6% CPS respectively. The basal diet was formulated to meet the nutritional requirements of birds according to NRC (1994). Feed and water was provided unrestricted and a completely randomized design and birds were vaccinated according to the prevailing disease in the environment. The results obtained showed that there were significant (p<0.05) differences among all treatments in the values of final live weight, body weight gain and feed conversion ratio. Diet containing 6% CPS had the highest weight gain of 2072.9 grams, while broilers fed 0% CPS had the lowest weight gain of 1534.7 grams. Supplementation of CPS did not affect (p>0.05) the daily feed intake and mortality rate. All the hematological (PCV, Hb, RBC, MCV, MCH, MCHC, WBC and its differentials) and serum biochemical parameters (Albumin, globulin, total protein, SGPT and SGOT) were not significantly (p>0.05) different among the treatments.
It can be concluded that the inclusion of CPS at 6% enhanced the overall performance of the birds without causing any deleterious effect on the health of the animals.

Keywords: Cantella asiatica leaf meal; Performance; Hematology, Serum biochemistry; Broilers.

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Alagbe, J.O. Effects of Dried Centella Asiatica Leaf Meal as a Herbal Feed Additive on the Growth Performance, Heamatology And Serum Biochemistry of Broiler Chicken. International Journal of Animal Research, 2019; 3:23.


1. Al-Bayati, F.A (2008). Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oil and methanol extracts. J. Ethnopharmacol. 116: 403-406.
2. Akerreta, S., Calvo, M.I., and Cavero, R. Y (2010). Ethnoveterinary knowledge in Navarra (Iberian Peninsula). J. Ethnopharmacol. 130:369-378.
3. Ahmed, A. A and Bassuony, N.I (2009). Importance of medical herbs in animal feeding. World Journal of Agricultural Science. 5: 456-465.
4. Ablise, M., Leininger Muller., C.D Wong, G. Siest, V. Loppinet and S. Visvikis (2004). Effects of thyme and oregano on growth performance of broilers from 4-42days of age and on microbial counts in crop, small intestine and caecum of broilers. Animal Feed Sci. Tech. 178:198-202.
5. H.A.A. Elagib and A.D.A. Ahmed (2011). Comparative study on haematological values of blood of indigenous chickens in Sudan. Asian Journal of Poultry Science 5(1): 41-45, 2011
6. Liu, Y.G., Yan, G., Chen G and Zhang, J (2009). Efficacy trials of crude extractions from Artemisia Annul. L against Newcastle disease virus in vivo in xinjiang. Mod. Appl. Sci. 3:176-178.
7. Rota, C., J.J Carraminana., Burillo, J and Herrera, A (2004). In vitro antimicrobial activity of essential oil from aromatic plants against selected food pathogens. Journal of Food Protection. 67:1252-1256.
8. Reitman, S. and Frankel, S (1957). A calorimetric method for the determination of serum glutamic oxaloacetate and serum glutamic pyruvic transaminase. Animal Journal of Clinical Pathology. 28-56.
9. Sokovic, M., Glamoclija, P.D., Bric, D (2010). Antibacterial effects of the essential oil of commonly consumed medicinal herbs using an In vitro Model Molecules. 15: 7532-7546.
10. Levic, J., I Cabarkapa., Todorovic, G., S. Pavkov., et al (2011). In vitro antibacterial activity of essential oil from plant family Lamiaceae. Romanian Biotech Lett. 16:6034-6041.
11. Latheef, S.K (2013). Evaluation of immunomodulatory properties of four herbal preparations (Withania somnifera, Tinospora cordifolia, Azadirachta indica and E Care Se Herbal) against chicken infectious anaemia in chicks. Msc Thesis, Deemed University, Indian Veterinary Research Institute, Izatnagar, UP, India.
12. Soetan, K.O., Akinrinde, A.S and AJibade T.O (2013). Preliminary studies on the haematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor) Pg 42-52. Proceedings of 38th Annual Conference of Nigerian Society of Animal Production.
13. Adeyinka, J.N and Bello, H.O (2013).Serum biochemical parameters in clinically healthy dogs in Ibadan. Tropical Veterinarian 16(3-4), 123-120.
14. Doumas, B.T and Briggs, H.G (1972) Serum Albumen Bromocresol Green Binding Standard Methods. Clinical Chemistry (7) 175-179.
15. Duncan, D.B. (1955). Multiple Range and Multiple F-Test Biometrics 11:1-42.
16. Harbone, I. B (1973) A guide to modern techniques to plant analysis. Chapman and hall, New York, USA 2nd Edition.
17. Scott, H.M (1965) Measurement of Albino Acid content of fish meal protein by chick growth assay, P.Sc, pp: 395-65.
18. A.O.A.C (2000). Association of Official Analytical Chemists. Official Methods of Analysis 19th Edition Washington, D.C Pp69-77.
19. Statistical Analysis Systems Institute Inc. (1997). SAS User guide statistic, version 6 edition. Gary, New York, USA.
20. Jain, N.C (1986) Schalms veterinary hematology 4th edition Lea and Febiger, Philadelphia
21. NRC, National Research Council (1994) Nutrient Requirement for Poultry (9th red) National Academy Press. Washington D. C, USA.
22. Singh, A. (2012). Managing internal parasites in organic livestock, Organic Agriculture Centre of Canada. http//www.cog.ca/documents/Managinginternalparasitesinorganiclivestock.pdf.
23. Chew Shio Heong., Kaur, Bhupinder.,Nurul Huda.,Alias A Karim and Ariffin Fazilah (2011). Effect of fermentation on the composition of Castella asiatica tea. American Journal of Food Tech. 6(7): 581-593.
24. Paramageetham, C., Babu, G.P and Rao, J.S (2004). Somatic enbrayogenesis in Castella asiatica L. an important medicinal and nutraceutical plant of India. Plant Cell Tissue Cult, 79:19-24.
25. Agbabiaka, L.A., Madubuike, F.N and Esonu, B.O (2012). Performance, carcass and organ weight characteristics of broiler finisher fed varying levels of tigernut meal as a dietary supplement. British Journal of Poultry Sciences. 1(2): 11-17, 2012.
26. Tarek M. Shafey., Ibrahim M. Al-Ruqaei and Saud I. Almufarij (2013). Effect of feeding Olive leaves extract on the performance, nutrient utilization, small intestine and carcass characteristics of broiler chickens. Journal of Animal and Veterinary Advances. 12(6): 740-746, 2013.
27. Hanan E. Al-Mashhadani (2015). Effect of different levels of turmeric supplementation on broiler performance, carcass characteristics and bacterial count. Egyptian Poultry Science Journal. Vol (35):1(25-39), 2015. http//www.epsaegypt.com
28. Abdi-Hachesoo, B., Talebi, A and Asri-Rezaei (2011). Comparative study on blood profiles of Indigenous and Ross-308 broiler breeders. Global Vet., 7:238-241.
29. Kececi, T and R. Col (2011). Haematological and biochemical values of the blood of pheasants of different ages. Turkish Journal of Veterinary Animal Science. 35: 149-156.
30. Karangiya1,V. V., H. H. Savsani1., Shrikant Soma Patil1., D. D. Garg1., K. S. Murthy1., N. K. Ribadiya1 and S. J. Vekariya (2016). Effect of dietary supplementation of garlic, ginger and their combination on feed intake, growth performance and economics in commercial broilers.Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.9/March-2016/4.pdf
31. Safa M.A. Eltazi., Mohamed, K. A., and Mukhtar, M.A (2014).Effect of using garlic powder as natural feed additive on performance and carcass quality of broiler chicks. Assiut Vet. Med. J. Vol. 60 No. 141 April 2014.
32. Simaraks, S.O., Chinrasri and W. Aengwanich (2004). Haematological electrolyte and serum biochemistry values of the Thai indigenous chickens (Gallus domesticus) in north eastern Thailand. Sonhklanakarin J. Sci. Technology, 26: 425-430.
33. Quintavalla, F., Bigliardi, E and Bertoni, P (2001). Blood biochemical baseline values in the Ostrich. Universita degli Studi di Parma Annali della Facolta di Med. Vet., 21:61-71. Ladokun, A. O., Yakubu, A., Otite, J.R., Omeje, J.N., Sokunbi, O.A and Onyeji, E (2008). Haematological and serum biochemical indices of naked neck and normally feathered. Nigeria indigenous chickens in a sub humid tropical environment. International Journal of Poultry Science. 7:55-58.
34. Talebi, A., Asri-Rezaei, S., Rozeh-Chai, R and Sahraei, R (2005). Comparative studies on haematological values of broiler strains (Ross, Cobb, Arbo-acre and Arian). International Journal of Poultry Science, 4:573-579.
35. Sarica, S., A. Ciftci., E. Demir, K. Kilinc and Y. Yildirim (2005). Use of an antibiotic growth promoter and two herbal natural feed additives with and without exogenous enzymes in wheat based broiler diets. South African Journal of Animal Science 2005, 35 (1), 2005.
36. Hargano, D., Astuti, Y., Lastari, P and Van Deh Bergh (1999). Centella asiatica : Plant resource of South east Asia Medicinal and Poisonous Plants; Padua L.S.N Bunyapraphatsara and R.H.M.J Lemmens (Eds) Backhuys Publisher Leiden, the Netherlands, Pp 190-194.
37. Mariappan Senthikumar (2018). Preliminary phytochemical screening of bioactive compounds from leaves of Centella asiatica L. Urban. International Journal of Biology Research, Vol 3; Issue 1; 2018; Page No. 57-65.
38. Loganathan, V., Devi Kaniakumari and Selvakumar, P (2017). A study of the physio-chemical and phytochemical parameters of leaves of Mallotus rhamnifolius. International Journal of Pharmacognosy and Phytochemical Research, 2017.
39. Bačkor, M., Kovacik, J., Piovar, J., Pisani, T and Loppi, S (2009). Physiological aspect of cadmium and nickel toxicity in lichens Peltigera rufescens and Cladina arbuscula Subsp. Mitis. Water, Air and Soil Pollution; doi: 10.100/s11270-009-0133-6.
40. Edeoga, H.O., Okwa, D.E., Mbaebie, B.O (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology, 4(7):685-688 (2005).
41. Tasneef Ahmad., Swatantra Bahadar., Singh, Shivshankar Pandey (2013). Phytochemical screening and physiochemical parameters of crude drugs. A brief Review: International Journal of Pharma Research & Review.
42. Umer Qadir, V.I Parkumar et al (2015). Phytochemical screening and In vitro bacterial activity of Anamirata cocculus (Linn) seeds. Journal of King Saud University-Science, 27(2), 97-104.
43. Subhashini, R., Mahadeva –Rao U.S., Sumathi P and Gunalan, G (2010). A comparative phytochemical analysis of cocoa and green tea. Indian Journal of Science and Tech. 3(2): 188-192.
44. Nagamani, J.E., Vidya Sri D., Syeda Hajira Bannu (2014). Antioxidant and antimicrobial properties of Bombax ceiba pentandra seed extract. World Journal of Pharmacy and Pharamaceutical Sciences. 3(12), 692-706.
45. Langhout, P (2000). New additives for broiler chicken. World Poultry 16: 22-27.
46. Adegoke, A. A., Iberi, P.A., Akinpelu, D.A., Aiyegoro, O.A and Mboto, C. I (2010). Studies on phytochemical screening and antimicrobial potential of Phyllanthus amarus against multiple antibiotic resistant bacteria. Int’l Journal of Appl. Res. Nat. Prod. 3(3), 6-12.
47. Deore, S.L., Khadabadi, S.S., Kamdi, K.S., Ingle, V.P., Kawalkar, N.G et al (2009). Anthelminthic activity of Cassia tora. International Journal of Chem. Tech. Res. 1(2), 177-179.
48. Shahid-Ud-Duaula, Anwarul, A.F.M and Basher (2009). Phytochemical screening and plant growth inhibition and antimicrobial activity studies of Xylocarpus granatum. Malaysian Journal of Pharmaceutical Science. 7(1), 9-21.
49. Hassan, M.M., Oyenwale, A.O., Abdullahi, M.S and Okonkwo, E.M (2004). Preliminary phytochemical and antibacterial investigation of crude extract of the bark of Datarium microcarpum. J. Chem. Soc. Nigeria.
50. Mohankumar, M and Lilitha (2015). In vitro anthelminthic activity of Hugonia mystax leaves Linn in Indian Adult Earthworm. Journal of Phramacognosy and Phytochemistry. 3(5), 19-21.
51. Chew, Y.L., Chan, E.W.L., Tan, P.L., Lim, Y.Y and Stanslas, J (2011). Assessment of phytochemical content, polyphenolic composition, antioxidant and antibacterial activities of Leguminosae medicinal plants in Peninsular Malaysia, BMC Complem. Alter. Med, 2011.
52. Senthilkumar, M., Srividhya, V and Mahalakshmi, D (2015). Phytochemical screening of bioactive compounds from Pleurotus ostreatus- A wild edible mushroom. World Journal of Pharmaceutical Research. 1603-1618.
53. Ghosh, A., Das, B.K., Chatterjee, S.K and Chandra, G (2008). Antibacterial analysis and phytochemical analysis of mature Polyalthia longifolia. South Pacific Journal Nat. Sci. 26:68-72.
54. Odebiyi, A and Sofowora, A. E (1978). Phytochemical screening of Nigerian medicinal plant. Part III Lloydia, 41, 234-246.
55. Mangalanayaki, R and Sivaneshwari, B (2018). Antibacterial activity and phytochemical analysis of Morinda tinctoria leaf extracts. International Journal of Biology Research; Page No. 198-201.
56. Suyogya, Shah, Kamana, Ghimire., Amit Kumar Gupta., Priyanka, Pokhrel., Janmajoy, Banerjee., Hermanta Khanal and Mahalaxmi Pradhananga (2017). Evaluation of phytochemical parameters, antibacterial and anthelminthic activity of leaves and bark extracts of plant Bombax ceiba. Jou. Appl. Pham. Res.
57. Sumaiya, Akhter., M.D Towhidul and Tanvir, Hossain (2015). Proximate, phytochemical screening and antioxidant activity of Tagetes erecta leaves. World Journal of Pharmaceutical Research.
58. Jaya Kumar D, Jaya Santhi R (2012). Antioxidant and cytotoxic effects of hexane extracts of Morinda pubcesens leaves in human liver cancer cell line. Asian Pacific Journal of Tropical Medicine.
59. Goyal, R.K., Garud, A and Garud, N (2013). Antimicrobial activity and phytochemical screening of leaves extract of Morinda pubsecens Linn. Jou of Drug Delivery & Therapeutics. 3(6):84-86.
60. Vidyadhar, S., Saidulu, M., Gopal, T.K and Chamundeeswari, Umamaheswara Rao and David Banji (2010). In vitro anthelminthic activity of the whole plant of Enicostemma littorale. Int’l Jou. Appl. Bio & Pharm. Tech.1 (3) 1119-11125.