Evaluation of lectin extracted from Canavalia brasiliensis on the neuropsychomotor system of murine

Evaluation of lectin extracted from Canavalia brasiliensis on the neuropsychomotor system of murine

Gonçalves, A.L.N1, Souza, A.V.F2, Borges, B.Q.O.V3, Paiva, J.C.G4, Morais, L.A5, Souza, M.B.R6

1Estudante do Curso de Medicina – UNICAP;
2,3,4,5Estudante do Curso de Medicina – UNICAP;
6Docente do Curso de Medicina – UNICAP

International Journal of Biomedical Engineering Research

Introduction: Lectins are a class of proteins extracted from the seeds of Canavalia brasiliensis (Lectin ConBr). Its anti-neoplastic activity has been studied in experimental models. Aim: To evaluate the neuropsychomotor effect of Lectin ConBr in vivo in murine. Methods: Thirty Swiss mice were divided into three groups: GCcontrol (n = 10) administered saline solution; G1experimental (n = 10) 50mg/kg and G2experimental (n = 10) 300mg/kg of Lectin ConBr. The neuropsychomotor analysis was performed by The Open Field Test, 4 and 24 hours after treatment. The statistic was performed by Student’s t-test. Results and Discussion: In the CG, the results obtained after 4h were: ambulation 55.4 ± 16.8; self-cleaning 4.8 ± 1.3; lifting 21.5 ± 3.41 and defecation 0.6 ± 0.5. G1: ambulation 43.4 ± 29.11; self-cleaning 3.4 ± 1.6; lifting 8.0 ± 8.1 and defecation 0.2 ± 0.44. G2: ambulation 63.0 ± 14.58; self-cleaning 1.0 ± 0.7; lifting 12.8 ± 3.1 and defecation 0.2 ± 0.44. The results obtained after 24 hours were: GC: ambulation 62.2 ± 27.9; self-cleaning 2.6 ± 1.5; lifting 15.3 ± 5.8 and defecation 1.4 ± 0.5. G1: ambulation 32.0 ± 15.7; self-cleaning 1.8 ± 1.7; lifting 6.0 ± 6.0; defecation 0.6 ± 0.5. G2: ambulation 62.0 ± 30.1; self-cleaning 0.6 ± 0.5; lifting 16.8 ± 7.3 and defecation 0.4 ± 0.5*. ConBr did not alter the spontaneous movement of the mice after 4 hours, however, the self-cleaning act and the total number of lifting in the treated groups decreased. After 24 hours, it was observed that self-cleaning decreased at the dose of 300mg/kg, lifting was decreased at a dose of 50mg/kg and the number of fecal cakes was decreased at all doses, suggesting anxiolytic action without motor impairment. Conclusion: The results indicate an anxiolytic effect of ConBr on treated animals when compared to the control group.

Keywords: Mannose-Binding Lectin; Muridae; Acute Toxicity

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How to cite this article:
Almeida, L.R.B; Silva Júnior, P.R; Martins, G.V.F; Oliveira, T.K.B; Araújo, D.A.M; Arruda, I.T.S. Evaluation of lectin extracted from Canavalia brasiliensis on the neuropsychomotor system of murine.International Journal of Biomedical Engineering Research, 2018, 1:4. DOI: 10.28933/ijber-2018-05-1804

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