Population Dietary Variation of Drosophila Melanogaster Associated with Different Yeasts


Population Dietary Variation of Drosophila Melanogaster Associated with Different Yeasts


Matheus Alves Siqueira de Assunção1*, Marcos Vinícius da Silva Alves de Lima1, Maria Eduarda dos Santos Pereira De Oliveira1, Bruna Zirpoli Leite Parisi1, Natália Maria de Souza Castro1, Igor Cesar De Oliveira Cirilo1, Fálba Bernadete Ramos Anjos2

1Graduates of the Biological Sciences course – Bachelor’s Degree, Biological Sciences Center, Federal University of Pernambuco (UFPE), 2Professor of the Federal University of Pernambuco (UFPE).


International Journal of Biomedical Engineering Research

Objective: To analyze the importance of the quality of the growth medium as to the development of Drosophila melanogaster grown in the laboratory, in order to assess changes in physical fitness and in the survival and fertility rates of the studied populations, both of the White and wild strains, according to the yeast. provided as a food resource.

Method: To do this, different yeasts were used as food resources (Saccharomyces cerevisiae, Dekkera bruxelensis and Meyerozyma caribbica) in the standard medium and in order to evaluate the variables, the RING test and analysis of survival and fertility rates were performed.

Results: Through the data, the growth medium with Saccharomyces cerevisiae was that with all analyzed rates were within the expected value and that the individuals were able to complete their life cycle, while the other resources did not obtain the expected values.

Conclusion: The efficiency of Saccharomyces cerevisiae for maintenance medium is confirmed, in addition to this fact, with the intriguing result of the other two yeasts, it is necessary to add different evaluation methods so that the results become increasingly robust.


Keywords: Drosophila melanogaster; growth medium; yeast; resource.


Free Full-text PDF


How to cite this article:
Matheus Alves Siqueira de Assunção, Marcos Vinícius da Silva Alves de Lima, Maria Eduarda dos Santos Pereira De Oliveira, Bruna Zirpoli Leite Parisi, Natália Maria de Souza Castro, Igor Cesar De Oliveira Cirilo, Fálba Bernadete Ramos Anjos.Population Dietary Variation of Drosophila Melanogaster Associated with Different Yeasts. International Journal of Biomedical Engineering Research, 2020, 3:7. DOI: 10.28933/ijber-2020-03-1005


References

1. BASS TM, GRANDISON RC, WONG R, MARTINEZ P, PARTRIDGE L AND PIPER MDW (2007) Optimization of dietary restriction protocols in Drosophila. Gerontol A Biol Sci Med
Sci 62: 1071-1081
2. BERNARDES, L.M.M. Validação de diferentes meios de cultura para estudos da progressão da doença de Alzheimer em Drosophila melanogaster. 2018. Monografia (Obtenção do grau de Bacharel em Biotecnologia) – Curso de Biotecnologia, Universidade Federal de Uberlândia, Minas Gerais, 2018
3. BREWER, 2002, BREWER, C. Conservation education partnerships in schoolyards laboratories: a call back to action. Conserv. Biol., Malden, v. 16, n. 3, p. 577-579, 2002.
4. CHAPMAN, 1998, CHAPMAN, R. F. The insects- Structure and Function. 4th. ed. Nova York: Cambridge University Press, 1998, p.69-93.
5. DANCHIN, E. 2013.Avatars of information: towards an inclusive evolutionary synthesis. Trends Ecol. Evol. 28: 351-358.
6. David, C., 1988, David, J. R.,P. Capy, p. 1988. Genetic variation of Drosophila melanogaster natural populations. Trends Genet. 4: 106-111.
7. Gilchrist & Huey 2004- Gilchrist, A. S. & R. B. Huey. 2004. Plastic and Genetic Variation in Wing Loading as a Function of Temperature Within and Among Parallel Clines in Drosophila subobscura. Integrative and Comparative Biology 44: 461-470.
8. GOMES, R. A. P. L. 2001. “Protocolo – Utilização de Drosophila em Genética: 1a Parte”. Dep. Biologia Vegetal, Faculdade de Ciências da Universidade de Lisboa, 2001.
9. HOFFMANN, A.A., SHIRRIFFS, J 2002. Geographic variation for wing shape in Drosophila serrata. Evolution 56: 1068-1073.
10. http://www.ordembiologos.pt/Publicacoes/Biologias/Droshort%20–%2001Jan01.pdf
11. HUEY, R.B., GILCHRIST, M.L., CARLSON, BERRIGAN, D., SERRA, L. 2000. Rapid evolution of a geographic cline in size in an introduced fly. Science 287: 308- 309.
12. MATA, R.A., McGeoch, M., TIDON, R. 2010. Drosophilids (Insecta, Diptera) as Tools for Conservation Biology. Natureza & Conservação 8: 60-65.
13. MAGWERE T, CHAPMAN T AND PARTRIDGE L (2004) Sex Differences in the Effect of Dietary Restriction on Life Span and Mortality Rates in Female and Male Drosophila Melanogaster. J Gerontol A Biol Sci Med Sci 59A (1):3-9.
14. MATTHEWS R.W. et al. Insects as teaching tools in primary and secondary education. Annu. Rev. Entomol., Palo Alto, v. 42, p. 269-289, 1997.
15. MORAIS, P.B., ROSA, C.A. 2000. Interações entre Drosophila e leveduras em ambientes tropicais. Pp. 321-336.
16. MÜLLER, G.B. 2013.Beyond Spandrels: Stephen J. Gould, EvoDevo, and the Extended Synthesis., pp. 85-99. InG. A. Danieli, A. Minelli &T. Pievani [eds.], Stephen J. Gould: The Scientific Legacy. Springer-Verlag, Milão, Itália.
17. PAPE T, BICKEL D, RUDOLF M (2009) Diptera Diversity: Status, Challenges and Tools. Leiden, Brill Academic Publishers. 459p.
18. PENNISI, E.Modernizing the Modern Synthesis. Science. 2008 321: 196-197.
19. PIGLIUCCI, M.G., MÜLLER, B., eds. 2010. Evolution: The Extended Synthesis. MIT Press, Cambridge, Massachusetts.
20. PIGLIUCCI, M. 2001. Phenotypic Plasticity: Beyond Nature and Nurture. The Johns Hopkins Uiversity Press, Baltimore.
21. PIPER, M.D.W, PARTRIDGE, L. (2007). Dietary restriction in Drosophila: Delayed aging or
experimental artefact? PLoS Genet 3(4): e57.
22. Sambucetti, P., V. Loeschcke & F. M. Norry. 2006. Developmental time and size- related traits in Drosophila buzzatii along an altitudinal gradient from Argentina. Hereditas 143: 77-83.
23. SCHOENER, T. W. The Newest Synthesis: Understanding the Interplay of Evolutionary and Ecological Dynamics. Science. 2011. 331: 426-429.
24. STEENSELS, J., DAENEN, L., MALCORPS, P., DERDELINCKX, G., VERACHTERT, H., VERSTREPEN, K. J. Brettanomyces yeasts — From spoilage organisms to valuable contributors to industrial fermentations. International Journal of Food Microbiology. 2015. 206, 24–38.
25. TENNEKES, H. 2009. The Simple Science of Flight: From Insects to Jumbo Jets. MIT Press, Cambridge, Massachusetts.
26. TIDON, R. Relationships between drosophilids (Diptera, Drosophilidae) and the environment in two contrasting tropical vegetations. Biological Journal of the Linnean Society. 2006. 87: 233-247.
27. WILLI, Y., HOFFMANN, A.A. Microgeographic adaptation linked to forest fragmentation and habitat quality in the tropical fruit fly Drosophila birchii. Oikos. 2012. 121: 1627-1637.
28. WEST-EBERHARD, M. J. 2003. Developmental plasticity and evolution. Oxford Univ.Press, Oxford.
29. XIAO, C., ROBERTSON, R.M. Timing of locomotor recovery from anoxia modulated by the white gene in Drosophila Genetics. 2016 203, 787–797.