Interactive impacts of stressful factors and adaptive compounds and assessment of the oxidative defense system in multiple-stressed broad bean plants

Interactive impacts of stressful factors and adaptive compounds and assessment of the oxidative defense system in multiple-stressed broad bean plants

M. E. Younis*, A. M. S. Kazamel and M. F. A. El-Sakaan

Botany Department, Faculty of Science, University of Mansoura, Mansoura, Egypt

Field-grown plants may experience multiple abiotic stresses, rather than a single stress. In this study, interactive impacts of stressful factors and adaptive compounds in NaCl- and Cd-stressed broad bean plants were investigated.
Proline as well as H2O2 contents, lipid peroxidation and electrolyte leakage were increased in NaCl- and/or Cd-treated plants, throughout the experiment, whereas sole application of AsA and SA to the unstressed plants led to a decline in the levels of these named parameters. Moreover, a further decline set in upon combination of the adaptive compounds with the stressful factors.
Anthocyanin contents fluctuated in different stress levels and single application of AsA or SA reduced anthocyanins; with additional reductions being induced when these compounds were supplemented with NaCl or Cd. Flavonoids and total phenols were increased in response to NaCl and/or Cd treatment; AsA and SA led to a decline or non-significant changes in flavonoids and total phenols, respectively, whereas the treatment of NaCl- and Cd-stressed plants with AsA or SA induced a marked increase, as compared with the stressed plants. In relation to control plants, all treatments adopted elicited significant increases in AsA and GSH contents.
All activities of the antioxidant enzymes were variably increased, throughout the experiment, in plants treated with NaCl and/or Cd. Application of adaptive compounds to the unstressed plants also led to significant increases in all the determined activities. On the other hand, addition of these compounds to the stressed plants, in general, significantly decreased the activities of the antioxidant enzymes.

Abbreviations: H2O2 hydrogen peroxide; AsA ascorbic acid; GSH glutathione reduced; SA salicylic acid; MDA malondialdehyde; SOD superoxide dismutase; CAT catalase; APX ascorbate peroxidase; GR glutathione reductase; PPO polyphenol oxidase.

Keywords: Vicia faba, Lipid peroxidation, Electrolyte leakage, antioxidants, NaCl, Cd.

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M. E. Younis, A. M. S. Kazamel and M. F. A. El-Sakaan.Interactive impacts of stressful factors and adaptive compounds and assessment of the oxidative defense system in multiple-stressed broad bean plants .International Journal of Communications and Networks, 2019, 2:9


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