ANTICANDIDA ACTIVITY OF ETHANOL EXTRACT JERINGAU (Acorus calamus L) RHIZOME AGAINST Candida albicans ISOLATE REMOVABLE FULL DENTURE ACRYLIC


ANTICANDIDA ACTIVITY OF ETHANOL EXTRACT JERINGAU (Acorus calamus L) RHIZOME AGAINST Candida albicans ISOLATE REMOVABLE FULL DENTURE ACRYLIC


Felisha Febriane Balafif, Warta Dewi, Indrati

Department of Oral Biology, Faculty of Dentistry, Universitas Padjadjaran, Indonesia


American Journal of Geographical Research and Reviews

Background: Maintaining denture hygiene by brushing and soaking it in disinfectant can prevent Candida infections. Jeringau (Acorus calamus L) is an herbal plant that its rhizome extract has antifungal activity against Candida albicans.

Aim of study: This study aimed to measure whether the value of minimum inhibitory concentration (MIC50) of ethanol extract rhizome of Jeringau against C.albicans isolates the maxillary removable full denture acrylic.

Materials and methods: Rhizome of jeringau was prepared. In vitro, study was conducted laboratory experimentally with ten samples tested by serial dilution ethanol extract  rhizome of jeringau method with eight different concentrations. They were dropped to 0,1 ml C. albicans and incubated at 37 oC for 24 hours and repeated twice. They were grown on Sabouraud Dextro Agar medium that were incubated and seen its growth.

Results: Inhibition zone extract of rhizome jeringau concentration 100% on plate I (9,4 mm) and plate II (8,85 mm). MIC50 of ethanol extract rhizome of  jeringau is 2,5 mg/ml.

Conclusion: Rhizome of jeringau containing β-asarone which is the major active component of antifungal against C. albicans. It was terpenoid group. Terpenoid caused porin damage. When porin was broken it would reduce the permeability of fungal cell wall resulting in fungal cells would lack of nutrients, thus the growth of fungi was inhibited


Keywords: Candida albicans, ethanol extract, Jeringau

Free Full-text PDF


How to cite this article:
Felisha Febriane Balafif, Warta Dewi, Indrati. ANTICANDIDA ACTIVITY OF ETHANOL EXTRACT JERINGAU (Acorus calamus L) RHIZOME AGAINST Candida albicans ISOLATE REMOVABLE FULL DENTURE ACRYLIC . International Journal of Dental Research and Reviews, 2019, 2:24. DOI:10.28933/ijdrr-2019-07-2205


References:

1. Glick M. Burket’s oral medicine. 12nd Edition. USA: People’s Medical Publishing House; 2015.
2. Millsop JW, Fazel N. Oral candidiosis clinical in dermatology. J Clindermatol 2016;02:022.
3. Puryer James. Denture stomatitis, a clinical update. Dent Update 2016;43:529-535.
4. Bianchi CMPC, Bianchi HA, Tadano T, Paula CR, Hoffmann-Santos HD, Leite, Hahn. Factors related to oral candidiasis in elderly users and non users of removable dental protheses. Rev Inst Med Trop Sao Paulo 2016;58:17.
5. Jonathan PW, Jennifer, Donald. Journal of prosthodontic on complete and removable denture. John. Wiley Blackweel 2018:131-132.
6. Ibrahim I, Ferry J, Prima L, Dinis P. Pengaruh lama perendaman dalam larutan chlorhexidine terhadap perubahan warna resin akrilik heat cured. JMKG 2016;5(1): 7-14.
7. Kumar SN, Aravind SR, Sreelekha TT, Jubi J, Dileep. Asarones from Acorus calamus in combination with azoles and amphotericin B: a novel synergistic combination to compete against human pathogenic Candida spesies in vitro. Appl Biochem Biotechnol 2015.
8. Cappucino JG, Natalie S. Microbiology: a laboratory manual. Pearson 2014.
9. Rajput SB, Mohan S. β-asarone, an active principle of Acorus calamus rhizome, inhibits morphogenesis, biofilm formation and ergosterol biosynthesis in Candida albicans. Phytomedicine J 2013;20:139-142.
10. Hemamalini, Rajarajan, Duraiselvi, Anandhalakshmi. Evaluation of antifungal properties of Acorus calamus L. Inter J of Current Research 2015;7:11825-11828.
11. Rita WS, Retno K, I Made DS. The essential oil contents of jeringau (Acorus calamus L.) rhizomes and their antifungal activity against Candida albicans. Journal of Health Sciences and Medicine 2017; 1:33-38.