Positive Effect of Injectable Platelet Rich Fibrin (i-PRF) on Vital Bone Formation in Graft Reconstruction of the Mandible: A Histologic and Histomorphometric Study


Positive Effect of Injectable Platelet Rich Fibrin (i-PRF) on Vital Bone Formation in Graft Reconstruction of the Mandible: A Histologic and Histomorphometric Study


1Cameron Y. S. Lee, DMD, MD, PHD, MPH, 2Hari Prasad, BS, MDT, MS, 3Cameron C. Y. Lee, DMD, MD, 4Sanjana Prasad, BDS, 5Jon B. Suzuki, DDS, PHD, MBA

1Private Practice in Oral, Maxillofacial and Reconstructive Surgery. Aiea, Hawaii 96701; Clinical Professor of Surgery, Department of Periodontology/Oral Implantology Kornberg School of Dentistry. Temple University, Philadelphia, PA 19140;2Assistant Research Director and Senior Research Scientist. Hard Tissue Research Laboratory. University of Minnesota School of Dentistry. Minneapolis. MN. 55455;3Oral and Maxillofacial Surgery Resident. Harvard Dental School. Boston, MA 02114;4Assistant Professor. Hard Tissue Research Laboratory. University of Minnesota School of Dentistry. Minneapolis, MN.55455; 5Professor Emeritus. Departments of Periodontology/Oral Implantology and Microbiology/Immunology. Temple University School of Dentistry and Medicine. Temple University. Philadelphia, PA 19140


American Journal of Geographical Research and Reviews

In this clinical case series, relative centrifugation force (RCF) and centrifugation time (CT) were reduced to produce the liquid form of platelet rich fibrin (i-PRF). The goal of this study is to histologically evaluate if the liquid form of PRF has a positive effect on vital bone formation in the grafted mandible using mineralized freeze-dried allogeneic bone.

Materials and Methods: Forty-two patients completed immediate bone grafting after a non-restorable endodontically treated molar tooth was extracted in preparation for future implant surgery. Each bone graft was augmented with i-PRF. To process i-PRF, relative centrifugation force (RCF) was reduced from 2,700 RPM to 700 RPM (60g). Centrifugation time was reduced from 12 minutes to 3 minutes. After an average bone graft healing time of 8 to 12 weeks, bone core samples were obtained at the time of implant surgery for histological and histomorphometric analysis.

Results: Forty-two bone cores were harvested at the time of implant surgery for histological and histomorphometric analysis. The liquid form of PRF resulted in greater percentages of vital bone formation (average 87.4 %) compared to the existing published literature.

Conclusion: i-PRF resulted in greater percentages of new vital bone formation compared to the published literature. This could be due to the continuous slow release and delivery of growth factors in i-PRF. Further clinical use of iPRF is needed to evaluate the full potential of bone regeneration.


Key Words: Injectable Platelet Rich Fibrin (i-PRF). Bone formation. Allogeneic bone. Reduction of Relative Centrifugation Force (RCF). Reduction of Centrifugation Time (CT). Histology. Histomorphometric analysis.

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How to cite this article:

Cameron Y. S. Lee, Hari Prasad, Cameron C. Y. Lee, Sanjana Prasad, Jon B. Suzuki. Positive Effect of Injectable Platelet Rich Fibrin (i-PRF) on Vital Bone Formation in Graft Reconstruction of the Mandible: A Histologic and Histomorphometric Study. International Journal of Dental Research and Reviews, 2020, 3:43. DOI: 10.28933/ijdrr-2020-05-2805


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