Research Article of International Journal of Nanoparticle Research
Cytotoxicity Studies on Naproxen and Piroxicam Nanoformulations
Sandeep Patnaika,b L.A. Avinash Chunduria,b, Aditya Kurdekarb , Venkataramaniah Kamisettib*
aAndhra Pradesh Medtech Zone Ltd., Vishakhapatnam, India
bLaboratories for Nanoscience and Nanotechnology(LNAN) Research, Department of Physics, Sri Sathya Sai Institute of Higher Learning, Prasanthinilayam, India
Caco-2 cells were used as in vitro models to assess the cell viability characteristics of the carriers Soluplus®, Gelucire 50/13 and PVP K25 and the nanoformulations of Naproxen and Piroxicam. The assessment of cell viability was done using the tetrazolium salt based MTT assay. Gelucire 50/13 and its NFs were observed to have slightly higher cytotoxicity than PVP and Soluplus® and their respective NFs. All the NFs were observed to follow the cytotoxicity trend of the polymers. Our results show that no significant decrease in cell viability was seen until 0.01% concentration of Gelucire 50/13 for 12-h exposure. The NFs as well as the polymers alone had no significant effect on the viability of Caco-2 cells below 0.01% concentrations. The intestine has a protective mucous layer, whereas the cell culture monolayers do not. The intestinal tissues also have more capacity to recover from trauma than the cultured cells. Hence the present NFs can be expected to show lesser cytotoxicity when subjected to in vivo studies.
Keywords: Caco-2 cells, Cytotoxicity, Nanoformulations, Polymers, MTT assay
How to cite this article:
Sandeep Patnaik, L.A. Avinash Chunduri, Aditya Kurdekar, Venkataramaniah Kamisetti.Cytotoxicity Studies on Naproxen and Piroxicam Nanoformulations. International Journal of Nanoparticle Research, 2017; 1:1. DOI: 10.28933/ijnr-2017-11-2501
1. Scherer, W. F., Syverton, J. T. & Gey, G. O. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J. Exp. Med. 97, 695–710 (1953).
2. Norrby, E. & Prusiner, S. B. Polio and nobel prizes: Looking back 50 years. Ann. Neurol. 61, 385–395 (2007).
3. Fogh, J., Fogh, J. M. & Orfeo, T. One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice. J Natl Cancer Inst 59, 221–226 (1977).
4. Sambuy, Y. et al. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol. Toxicol. 21, 1–26 (2005).
5. Arturssona, P., Palm, K. & Luthmanb, K. Caco-2 monolayers in experimental and theoretical drug transport predictions of. (1996).
6. Artursson, P. & Borchardt, R. T. Intestinal drug absorption and metabolism in cell cultures: Caco-2 and beyond. Pharmaceutical Research 14, 1655–1658 (1997).
7. Johnson, S., Nguyen, V. & Coder, D. Assessment of cell viability. Curr. Protoc. Cytom. (2013). doi:10.1002/0471142956.cy0902s64
8, Riss, T. L. et al. Cell Viability Assays. Assay Guid. Man. 1–23 (2004). doi:10.1016/j.acthis.2012.01.006
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