International Journal of Animal Research


Molecular study for bovine herpes virus type 1 detection in Iranian cattle

Research Article of International Journal of Animal Research Molecular study for bovine herpes virus type 1 detection in Iranian cattle Rassoul Hashemzehi1, Ayse Kilic2, Arman Akbarpour3, Esmaeil Mahmoudi3, Asghar Arshi4* 1. Department of Genetics, Fars Science & Research Branch, Islamic Azad University, Shiraz, Iran 2. Sivrice Vocational High School, University of Firat, 23119 Elazig-Turkey 3. Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran 4. Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran Bovine herpes virus type 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis, is a DNA virus. This pathogen is represents the most common viral pathogen found in cattle semen. The aim of the present study was to set up a of BHV-1 detection assay in bovine blood in Lorestan province using PCR assay. The blood samples of 285 cattle in Khoramabad, Azna, Aligoodarz, Borujerd and Poldokhtar were collected, total DNA was extracted and the region encoded the gI glycoprotein was amplified by PCR using specific primers. Out of 285 blood samples, 56 (19.64%) were positive for BHV-1 (468 bp). The highest and lowest frequencies of the bacterial infection were observed in Khoramabad and Borujerd cities with 21 and 12%, respectively. The results of this study demonstrated that PCR assay represent an excellent (suitable) alternative or additional tool for BHV-1 isolates detection. Finally the study revealed a high incidence of BHV-1 in the blood of Iranian cattle. Thus all cattle must be tested periodically for BHV-1 infection and antimicrobial drugs, to prevent BHV-1 occurrence in cattle must be used. The cattle must be free BHV-1 infection prior to use. Keywords: Bovine herpes virus type 1, gI gene, PCR, Iran ...

Histological structure of Azerbaijan buffalo Uterus

Research Article of International Journal of Animal Research Histological structure of Azerbaijan buffalo Uterus Hamid Karimi1, Negar Mahdavi2, Sahar Shadi3 1-Associate Professor of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Tabriz 2-Veteriinary Histology MS Student, Faculty of Veterinary Medicine, University of Tabriz 3- Veterinarian Asian buffalo is economic animal for variant reasons in different regions of Iran. This animal either is important for reason of resistance against local disease or is important for reason of high production from low quality of food materials. Meanwhile, different and broad genetical, nutritional and structural studies are not performed on buffalo completely. Therefore this ruminant is unknown animal yet. Study of normal structure of genital system of this animal is very important for diagnosis of genital system disorders and enhancement calve and milk production. Melanocytes are a series cell which originated from skin basal layer cells. These cells are placing in skin basal layer and they are producing melanin pigment. 10 healthy buffalo uteruswere selected from Tabriz semiindusterial slaughterhouse. The selected uteruswere fixed by 10% formalin and then tissue sections were provided. Sections were stained by H&E method firstly and then by special staining method for melanocytes. This research results were showed which endometrium, preimetrium and myometrium can be observed in buffalo uterussimilar to other mammals. Because muscularis mucosa is not observed in tunica mucosa of buffalo uterus, lamina properia and tunica submucosa is united. Lamina properia and tunica submucosa is occupied by simple tubular and simple coild tubular gland. Also, based on this research results, many melanocytes is observed in Lamina properia and tunica submucosa of Azerbayjan buffalo. Existence melanocytes in buffalo uterusis not reported till this time. This report is the first report of this cell in this organ. Keywords: Buffalo, Histology, Melanocyte, Uterus ...

Isolation, Identification and Antimicrobial resistance profile of staphylococcus aureus and occurrence of methicillin resistant S. aureus isolated from mastitic lactating cows

Research Article of International Journal of Animal Research Isolation, Identification and Antimicrobial resistance profile of staphylococcus aureus and occurrence of methicillin resistant S. aureus isolated from mastitic lactating cows in and around Assosa town, Benishangul Gumuz Region, Ethiopia Yami Asmamaw Aki1, Kibeb Legesse2, Asmelash Tassew2* 1Assosa Regional Veterinary Laboratory, Assosa, Ethiopia 2Addis Ababa University, College of Veterinary Medicine and Agriculture, P.O.Box: 34; Debre Zeit, Ethiopia A cross - sectional study was conducted from November 2016 to May 2017 in Dairy cattle in and around Asossa town in order to estimate the prevalence of mastitis, to identify S. aurues from mastitic lactating cows, to evaluate its antimicrobial resistance pattern and to identify risk factors associated with mastitis. A total of 384 Dairy cows milk samples were collected using purposive sampling techniques. The overall prevalence of mastitis at cow level was 39.32 % with 11.45 % and 27.86 % of clinical and subclinical mastitis, respectively. In this study, the subclinical mastitis was significantly higher than clinical mastitis. For all except Age and parity, the multivariable logistic regression analysis for intrinsic and extrinsic risk factors showed significant difference in the prevalence of mastitis in the study area (P˂0.05). From 151 mastitis infected lactating cows, 436 milk samples were cultured and 22.14% S.aureus were isolated. Presumptively identified S. aureus isolates were subjected to antimicrobial susceptibility test and 63(74.11%) MRSA have been identified from a total of 85 S. aureus using cefoxitin through disk diffusion method. The present result showed a significant association of resistance pattern with S.aureus isolates, particularly to penicillin G (95.55%), Cefoxitin (77.19%), Tetracycline (63.41%), Streptomycin (60.78%), Gentamycin (59.37%), Vancomycin (56.75%), Clindamycine (54.35%) and Bacitracin (53.65%). In this study, 77.19 % S.aureus isolates were found resistant against Cefoxitin. There were also observed multidrug resistance, mainly to Penicillin G, Streptomycin and Tetracycline. The ...

Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia

Research Article of International Journal of Animal Research Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts, Benishangul Gumuz Regional State, Ethiopia Yami Bote, Kibeb Legesse, Asmelash Tassew Addis Ababa University, College of Veterinary Medicine and Agriculture P.O.Box: 34; Debre Zeit, Ethiopia A cross sectional study was conducted from November 2016 to May 2017 in Bambasi and Assosa districts in Benshangul Guzum Regional State with the objectives of isolation, identification and determination antimicrobial susceptibility profile of Pasteurella multocida isolated from cattle infected with Hemorrhagic Septicemia. The study was also aimed to detect the agent using classical Polymerase chain reaction. Specimens were collected purposively from cattle in areas with outbreak of Hemorrhagic septicemia and a total of 384 swab samples were collected aseptically from cattle and transported to Assosa Regional Veterinary Laboratory using a cold chain system. Specimens were cultured on MacConkey agar and blood agar. Different biochemical tests were also used in order to identify and characterize the agent of the suspected disease. The prevalence of P. multocida was 13 (3.39%) out of 384 samples tested). All the isolates were subjected to the in vitro antimicrobial ssensitivity tests and the result indicated the presence of different degrees of susceptibility and resistance to most of the antibacterial agents. The degree of susceptibility ranges from 15.4% for Tetracycline upto 61.5% for Sterptomycin, and resistance is between 15.4% for Sterptomycin, Clindamycin and Chloramphicol and 69.2% for Tetracycline. Similarly, (60 %) of this discs showed the presence of multidrug resistance. The result of this test clearly indicates that, Sterptomycin, Clindamycin and Chloramphicol could be prospective drugs of choice. Analysis of PCR assay revealed the presence of P. multocida serotype B2. In conclusion, the presence of P. multocida serotype B2 in the selected areas can be ...

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International Journal of animal research

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