Study the Role of Combined M2-Pyruvate kinase, Calprotectin and Occult Blood Test as Fecal Biomarkers for Early Detection of Colorectal Cancer


Study the Role of Combined M2-Pyruvate kinase, Calprotectin and Occult Blood Test as Fecal Biomarkers for Early Detection of Colorectal Cancer


Atef Abo El-Soud Ali1, Mohamed Gamal El Helbawy2, Huda Saad Elmehily3, Emad Mohamed Salem4, Ahmed Abo Zaid Ahmed5

1Proffessor of Tropical Medicine(Menoufia University), 2Lecturer of clinical Pathology(Menoufia University) 3Tropical Medicine Department (Menoufia University), 4Head of Gastroenterology department of NJCH ( New Jeddah Clinic Hospital ) KSA, 5Lecturer of Tropical Medicine(Menoufia University)


Open Journal of Gastroenterology and Hepatology

Background and study aim: One of the main requirements of biomarkers for detecting CRC is that it must allow detection of the disease at earlier stages. The current study is designed to investigate the role of combined M2-Pyruvate kinase, Calprotectin, and Fecal occult blood test measurements as fecal diagnostic biomarkers for early detection of colorectal cancer. Patients & Methods: Total number of 72 subjects (48 patients and 24 healthy controls) were included in the study. Patients with cancer colon and patients with organic non-malignant colorectal lesions were recruited from Oncology and Gastroenterology Outpatient Clinics and Inpatient Department Menoufia University Hospital. Results: There was a highly significant difference between studied groups regarding age and gender (P> 0.001), cancer colon was higher among old age (mean ± SD is 58.96±6.3) and males (70.8%), There was a highly significant difference between studied groups regarding Hb, CRP and ESR (P >0.001); lowest Hb level was detected in cancer group (8.7±1.7). On the other hands highest CRP and ESR levels were in cancer group (7.29±4.47) and (14.71±6.19) respectively. There was a significant difference between studied groups regarding platelets (P >0.05); lowest platelet number was detected in cancer group (272.4±66.9). There was non-significant difference between studied groups regarding WBCs (P <0.05); highest WBCs number was detected in cancer group (8.05±2.3), There was a highly significant difference between studied groups regarding colonoscopy (P >0.001), There was a highly significant difference between studied groups regarding M2-Pyruvate kinase and Occult Blood (P >0.001), There was highly significant difference between M2-PK in cancer and control groups (p >0.001), There was significant difference between calprotectin in cancer and control groups (p >0.05), There was highly significant difference between occult blood in cancer and control groups (p >0.001), There was highly significant difference between Combined M2-PK and Calprotectin in cancer and control groups (p >0.001), There was highly significant difference between Combined M2-PK and Occult blood in cancer and control groups (p>0.001), There was highly significant difference between Combined M2-PK, Calprotectin and occult blood in cancer and control groups (p >0.001). Conclusion: Our study confirms that, Colorectal Cancer (CRC) increases with age and it is more common in male, CRP and ESR increase in CRC patients, Fecal M2PK, FC and FOBT in stool increase significantly in CRC patients, Sensitivity, specificity and accuracy of screening for CRC are highest in combination of fecal M2PK, occult blood in stool and FC tests.


Keywords: M2-Pyruvate kinase, Calprotectin, Occult Blood Test, Fecal Biomarkers, Early Detection of Colorectal Cancer

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How to cite this article:
Atef Abo El-Soud Ali, Mohamed Gamal El Helbawy, Huda Saad Elmehily, Emad Mohamed Salem, Ahmed Abo Zaid Ahmed. Study the Role of Combined M2-Pyruvate kinase, Calprotectin and Occult Blood Test as Fecal Biomarkers for Early Detection of Colorectal Cancer .Open Journal of Gastroenterology and Hepatology, 2019, 2:21. DOI: 10.28933/ojgh-2019-11-2706


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