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Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.

Research Article of American Journal of Microbiology and Immunology Bacteruria Associated Urogenital Infections Amongst Women “a Study On Assymptomatic And Symptomatic Presentation In Benin Metropolis, Nigeria”.  IBEH.N.ISAIAH1 (BMLS, AIMLS, MNAAS) 1Department of Medical Microbiology, University of Benin Teaching Hospital, PMB 111 2Department of Microbiology, Faculty of Life Science, University Of Benin, PMB 1154, Introduction: Symptomatic and Asymptomatic urinary tract infection in women is of great importance to determine the causal and treatment of bacteruria in young women which may lead to disease states. Aim: to determine the occurrence of asymptomatic bacteriuria in young women and its relationship with urinary tract infection Methods: A microbiological analysis of urine was done on 3600 young women in urban Benin metroplolis from July 2009 to July 2010, a total of 1105 microorganism were isolated from both Symptomatic and Asymptomatic young women. Results: From the 1105 isolates from the descending order of occurrences Escherichia coli, Kleibseilla spp, Enterococcus faecalis and Staphylococcus aureus with Acinobacter spp with the least occurrence. Escherichia coli was the commonest cause of Asymptomatic urinary tract infection with 31.6 % and Acinectobacter sp 9.5% with the least occurrence of Asymptomatic urinary tract infections. Conclusion: This study shows that there is a rise in asymptomatic urinary tract infection amongst young women in Benin City. Keywords: BacterIuria, UTI, Asymptomatic ...

Management of Post Bilateral Pontine Haemorrhage with Ayurveda

Case report of Journal of Stroke Research Management of Post Bilateral Pontine Haemorrhage with Ayurveda Kajaria Divya Assistant Professor, Department of Kayachikitsa, C.B.P.A.C.S, New Delhi Background and Purpose. This case report documents the management of residual paralysis due to a rare and fatal disease – hemorrhagic leukoencephalitis with Ayurveda. The purpose of this report is to illustrate the role of Ayurvedic treatment methodology for management of such neurological disorders. Case Description: Mrs. PM was a 31 year old lady who suffered from an attack of acute hemorrhagic leukoencephalitis after her second delivery. She had left hemiplegia with right sided LMN facial palsy with nystagmus and loss of control of posture and whole-limb movements. Some residual ability to use the right hand and fingers remained, provided the trunk and right upper arm were stabilized. Her cognitive power remains undiminished. Computed tomographic scans verified acute bilateral paramedian pontine hemorrhage with intraventricular extension. It also showed chronic infarcts in bilateral external capsules. Discussion. 15 days treatment with Ayurvedic medicines and Panchakarma procedures including Nasya and Tarapan therapies showed significant improvement in nystagmus, postural abnormalities and whole – body movement. Keywords: Nasya, Tarpana, Nystagmus, Ataxia ...

Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR

Research Article of American Journal of Microbiology and Immunology Detection of methicillin resistant and slime factor production of coagulase negative Staphylococcus spp. in bovine clinical mastitis by using PCR S. M. El-Berbawy*; S.M. Sayed*;El-Toukhy, E. I. ** and Amal, A. Megahed*** * Assiut Lab. (Bacteriology Department) ** AHRI (Biotechnology Depart.) Dokki *** Port Said Lab. (Bacteriology Depart.) This study aims to investigate the slime production of Coagulase negative staphylococci (CoNS) isolates by phenotypic method on Congo Red Agar plates (CRA) and Genotypic detection of icaA, icaD and mecA genes by polymerase chain reaction (PCR). Out of 105 milk samples obtained from clinical bovine mastitis, 101samples (96.2%) were positive for bacterial growth. CoNS isolates was detected in 20 isolates with a percentage of 19.8%. Their ability to form biofilm as one of the most important virulence factors of the organisms using Congo Red Agar (CRA) method was investigated in which 13 out of 17 CoNS isolates (76.47%) were found to be slime producers. By PCR, mecA gene was found in threeout of 6 CoNS isolates (50%). Also six (100%) and three (50%) isolates were positive for icaA gene and icaD gene, respectively. In addition one isolate out of the six CoNS isolates (16.67%) was positive for the presence of icaA, icaD and mecA genes and also has the ability to form biofilm. The in vitro activities of CoNS against 11 selected antimicrobial agents referred that the highest resistance rate of CoNS observed to Lincomycin (100%), followed by Cefotaxime (94.41%), Oxacillin (58.82%), Ampicillin (47.06%) and Penicillin (41.18%), while the highest rate of sensitivity observed to Enrofloxacin and Gentamicin (100%, for each), followed by Doxycycline (94.11%).Conclusion, the findings of the present study demonstrated the ability of CoNS isolated from bovine clinical mastitis to form biofilms. This must be considered as an alarming situation, ...

The Accumulation Of Cadmium In Corn At Different Levels Of Soil Ph

Research Article of American Journal of Microbiology and Immunology The Accumulation Of Cadmium In Corn (Zea Mays L.) At Different Levels Of Soil Ph Rabah S. Shareef*1,2, Awang Soh Mamat*3, Zakaria Wahab*4, Ibni Hajar Rukunudin*5 1 College of Education-Al-Qiam, University of Anbar, Iraq. 2 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 3 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 4 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. 5 School of Bioprocess Engineering, University Malaysia Perlis, Perlis, Malaysia. A pot experiment was carried out in the plastic greenhouse at the Institute of Sustainable Agrotechnology (INSAT), University Malaysia Perlis in Sungai Chuchuh, Perlis, Malaysia. During season of the cultivation of 2014 investigate the impact of soil pH and cadmium on growth of corn plant (Zea mays L.). Twenty five were arranged in factorial experiments according to the Complete Randomized Design (CRD), with three replicates. Five levels of soil pH were, pH4, pH5.2 (i.e., the original value), pH6, pH7 and pH8 and five levels of cadmium (Cd); Cd 1, Cd 2, Cd 3, Cd 4 and Cd 0 where the amounts (2, 4, 6, 8 mg. kg-1 soil and control treatment without add cadmium) are applied as CdCl2. Thus, the total numbers of pots were 75 pots. The results of this investigation revealed that; The decrease of soil pH led to significant effect to increase concentration of cadmium in root, stem, leaves and grain of corn, where the level of pH 5.2 gave highest concentrations of Cd compared with pH 8. the interactions between soil pH and cadmium led to increase of reduced the accumulation of cadmium in the corn, where it gave the interaction between pH 8 and Cd 0 lowest concentration of cadmium in root, stem, leaves and grain. Keywords: Accumulation, Cadmium, Corn, Soil pH, ...

A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates

Research Article of American Journal of Microbiology and Immunology A contribution on Coliforms causing mastitis in cows with reference to serotypes and virulence factors of E. coli isolates Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) Escherichia coli (E. coli) is the predominant coliform species causing intramammary infections. Where in the present study, E. coli isolates were 18 strains (17.82%) followed by Enterobacter aerogenes 3 strains (2.97%) and Klebsiella pneumoniae one strain (0.99%) from 101 clinical mastitic milk samples of cows. Eighteen E. coli isolates were serotyped to nine different serogroups; O111:H4 (3), O127:H6 (3), O26 (2), O126 (2), O119:H6 (1), O114:H21 (1), O55:H7 (1), O44:H18 (1), O124 (1) and (3) untyped. Virulence tests were performed on the 18 isolated E. coli, it was found that 15 isolates (83.3%) were serum resistant, 13 isolates (72.2%) had Congo Red binding activity, 6 isolates (33.3%) were invasive and one isolate (5.6%) had haemolytic activity. PCR was applied to detect the presence of Shiga like toxin producing E. coli (stx1 and stx2 genes) on the nine different strains (one strain for each serogroup), where stx1 and stx2 were found in 8 (88.9%) and 4 (44.4%) of the nine examined strains, respectively. While stx1 and stx2 genes were found together in 3 strains (33.3%). Conclusions:  E. coli isolates usually posses one or more virulence factors that may help in establishment at the infection site and subsequently causing clinical bovine mastitis. Keywords: Coliforms, E. coli, serotypes, virulence factors, stx1 and stx 2 ...

Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA)

Research Article of American Journal of Microbiology and Immunology Bacteriological study on staphylococcal bovine clinical mastitis with reference to methicillin-resistant Staph. aureus (MRSA) Sayed, S. M. Egypt- Animal Health Research Institute (Assiut Regional Lab., Bacteriology Dept.) This descriptive study was done on 101 milk samples obtained from clinically mastitic dairy cows in Assiut Governorate, Egypt. Staphylococcus aureus (S. aureus) was the main causative agent of clinical mastitis (34.65%) followed by S. saprophyticus (10.89%), S. intermedius and S. epidermidis (8.91%, for each). The other causative agents (non Staph. Spp.) were identified. Sensitivity test of S. aureus isolates was performed against 11 antimicrobial agents, where found that 21 S. aureus strains (60%) were methicillin resistant S. aureus (MRSA). Ten MRSA strains were subjected for: I- slime-producing factor on Congo Red Agar (CRA) plates phenotypically, as 6 isolates (60%) were positive for slime production. II- PCR which was optimized targeting mecA, icaA and icaD genes, where 5 isolates (50%) were positive for mecA gene. Six isolates (60%) and 8 isolates (80%) were positive for icaA and icaD genes, respectively. Five strains (50%) were positive for both icaA and icaD genes. Also 3 strains (30%) were positive for all mecA, icaA and icaD genes. Conclusion, it was concluded that bovine staphylococcal mastitis was the most predominant issue where S. aureus was the main cause. Detection of mecA gene in S. aureus isolates indicating that several cases suffering from S. aureus mastitis have an MRSA problem. Genotypic determination of mecA gene proved the most reliable method for detection of methicillin-resistant S. aureus. The present work paid an attention to the 3 MRSA strains (30%) were positive to all tested genes rather than slime production as the worst isolated strains all over this study (multidrug resistant, slime producing as well as carrying mecA, icaA and icaD genes). In vitro Enrofloxacin, Gentamicin and Doxycycline the most effective drugs for Staph. spp. clinical mastitis and should be recommended for treatment of such cases of bovine mastitis.    Keywords: Cows, clinical mastitis, methicillin-resistant Staphylococcus aureus, ...