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Histological structure of Azerbaijan buffalo Uterus

Research Article of International Journal of Animal Research Histological structure of Azerbaijan buffalo Uterus Hamid Karimi1, Negar Mahdavi2, Sahar Shadi3 1-Associate Professor of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Tabriz 2-Veteriinary Histology MS Student, Faculty of Veterinary Medicine, University of Tabriz 3- Veterinarian Asian buffalo is economic animal for variant reasons in different regions of Iran. This animal either is important for reason of resistance against local disease or is important for reason of high production from low quality of food materials. Meanwhile, different and broad genetical, nutritional and structural studies are not performed on buffalo completely. Therefore this ruminant is unknown animal yet. Study of normal structure of genital system of this animal is very important for diagnosis of genital system disorders and enhancement calve and milk production. Melanocytes are a series cell which originated from skin basal layer cells. These cells are placing in skin basal layer and they are producing melanin pigment. 10 healthy buffalo uteruswere selected from Tabriz semiindusterial slaughterhouse. The selected uteruswere fixed by 10% formalin and then tissue sections were provided. Sections were stained by H&E method firstly and then by special staining method for melanocytes. This research results were showed which endometrium, preimetrium and myometrium can be observed in buffalo uterussimilar to other mammals. Because muscularis mucosa is not observed in tunica mucosa of buffalo uterus, lamina properia and tunica submucosa is united. Lamina properia and tunica submucosa is occupied by simple tubular and simple coild tubular gland. Also, based on this research results, many melanocytes is observed in Lamina properia and tunica submucosa of Azerbayjan buffalo. Existence melanocytes in buffalo uterusis not reported till this time. This report is the first report of this cell in this organ. Keywords: Buffalo, Histology, Melanocyte, Uterus ...

Isolation, Identification and Antimicrobial resistance profile of staphylococcus aureus and occurrence of methicillin resistant S. aureus isolated from mastitic lactating cows

Research Article of International Journal of Animal Research Isolation, Identification and Antimicrobial resistance profile of staphylococcus aureus and occurrence of methicillin resistant S. aureus isolated from mastitic lactating cows in and around Assosa town, Benishangul Gumuz Region, Ethiopia Yami Asmamaw Aki1, Kibeb Legesse2, Asmelash Tassew2* 1Assosa Regional Veterinary Laboratory, Assosa, Ethiopia 2Addis Ababa University, College of Veterinary Medicine and Agriculture, P.O.Box: 34; Debre Zeit, Ethiopia A cross - sectional study was conducted from November 2016 to May 2017 in Dairy cattle in and around Asossa town in order to estimate the prevalence of mastitis, to identify S. aurues from mastitic lactating cows, to evaluate its antimicrobial resistance pattern and to identify risk factors associated with mastitis. A total of 384 Dairy cows milk samples were collected using purposive sampling techniques. The overall prevalence of mastitis at cow level was 39.32 % with 11.45 % and 27.86 % of clinical and subclinical mastitis, respectively. In this study, the subclinical mastitis was significantly higher than clinical mastitis. For all except Age and parity, the multivariable logistic regression analysis for intrinsic and extrinsic risk factors showed significant difference in the prevalence of mastitis in the study area (P˂0.05). From 151 mastitis infected lactating cows, 436 milk samples were cultured and 22.14% S.aureus were isolated. Presumptively identified S. aureus isolates were subjected to antimicrobial susceptibility test and 63(74.11%) MRSA have been identified from a total of 85 S. aureus using cefoxitin through disk diffusion method. The present result showed a significant association of resistance pattern with S.aureus isolates, particularly to penicillin G (95.55%), Cefoxitin (77.19%), Tetracycline (63.41%), Streptomycin (60.78%), Gentamycin (59.37%), Vancomycin (56.75%), Clindamycine (54.35%) and Bacitracin (53.65%). In this study, 77.19 % S.aureus isolates were found resistant against Cefoxitin. There were also observed multidrug resistance, mainly to Penicillin G, Streptomycin and Tetracycline. The ...

Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia

Research Article of International Journal of Animal Research Isolation, identification and antimicrobial susceptibility of Pasteurella multocida from cattle with hemorrhagic septicemia in Assosa and Bambasi districts, Benishangul Gumuz Regional State, Ethiopia Yami Bote, Kibeb Legesse, Asmelash Tassew Addis Ababa University, College of Veterinary Medicine and Agriculture P.O.Box: 34; Debre Zeit, Ethiopia A cross sectional study was conducted from November 2016 to May 2017 in Bambasi and Assosa districts in Benshangul Guzum Regional State with the objectives of isolation, identification and determination antimicrobial susceptibility profile of Pasteurella multocida isolated from cattle infected with Hemorrhagic Septicemia. The study was also aimed to detect the agent using classical Polymerase chain reaction. Specimens were collected purposively from cattle in areas with outbreak of Hemorrhagic septicemia and a total of 384 swab samples were collected aseptically from cattle and transported to Assosa Regional Veterinary Laboratory using a cold chain system. Specimens were cultured on MacConkey agar and blood agar. Different biochemical tests were also used in order to identify and characterize the agent of the suspected disease. The prevalence of P. multocida was 13 (3.39%) out of 384 samples tested). All the isolates were subjected to the in vitro antimicrobial ssensitivity tests and the result indicated the presence of different degrees of susceptibility and resistance to most of the antibacterial agents. The degree of susceptibility ranges from 15.4% for Tetracycline upto 61.5% for Sterptomycin, and resistance is between 15.4% for Sterptomycin, Clindamycin and Chloramphicol and 69.2% for Tetracycline. Similarly, (60 %) of this discs showed the presence of multidrug resistance. The result of this test clearly indicates that, Sterptomycin, Clindamycin and Chloramphicol could be prospective drugs of choice. Analysis of PCR assay revealed the presence of P. multocida serotype B2. In conclusion, the presence of P. multocida serotype B2 in the selected areas can be ...

Socio-cultural, Herd Structure and Reproductive Practices of Pastoral Cattle Producers in Adamawa State, Nigeria

Research Article of International Journal of Animal Research Socio-cultural, Herd Structure and Reproductive Practices of Pastoral Cattle Producers in Adamawa State, Nigeria I.  H.  KUBKOMAWA1, M. S. ADAMU1,  M.  A.  OGUNDU2 , I.  C. OKOLI2 and  A. B. I.  UDEDIBIE2 1. Department of Animal Production and Health, Federal Polytechnic, Pmb 35, Mubi, Adamawa State, Nigeria 2. Department of Animal Science and Technology, Federal University of Technology Owerri, Imo State, Nigeria The objectives of the study were to investigate the socio-cultural, herd structure and reproductive practices of pastoralist cattle producers in Nigeria. The study was carried out with the aid questionnaires, oral interview and field observations on 300 respondents spread across the three study LGAs. Pastoral cattle production in Adamawa state was predominated by highly experienced (80 – 85%), married (75 - 88%), male (75 – 90%) Fulani (95 – 65%) Muslims (75 – 80%) aged mostly 31 – 40 years (48 – 55%) and having limited western education. White Fulani (50.00%) was the most common breed in Gombi LGA, while Red Bororo (53.00%) and Adamawa Gudali (50.00%) were the most predominant breeds in Mubi North and Jada LGAs respectively. Most of the pastoralist (40 – 50%) maintained herd size of 41 to 50 heads and reared cattle for multiple purposes such as breeding, milk, meat and traction. Farmers practiced uncontrolled breeding, with bull to cow ratio of 1:10 (75.00% at Mubi north LGA). First mating (50 – 60%) was done between 4 and 5 years, while age at first calving (73 -75%) was mostly 5 – 7 years indicating serious reproductive life wastage. Most pastoralists (55 – 65%) use ethno-veterinary practices to enhance cattle reproductive performance. Calving rates (75 – 85%) were more during late rainy season (LRS), while (90.00%) depended on natural pastures for feeding their cattle. Cattle graze ...

Influence of Contamination of Soil With Heavy Metal on the Growth of Three Herbaceous Plant Species

Research Article of American Journal of Agricultural Research Influence of Contamination of Soil With Heavy Metal on the Growth of Three Herbaceous Plant Species 1C.E., Igwe, 2J.N., Azorji and 2S.K., Iheagwam 1Department of Forestry and Environmental Management, Michael Okpara University of Agriculture, Umudike, Nigeria 2Department of Biological Sciences, Hezekiah University, Umudi, P.M.B 3002 Ishiala Umudi, Imo State, Nigeria. Contemporary literatures are awash with Heavy metal contamination in soils as a source of global environmental concern. Cultivation of crops on these contaminated soils may result in accumulation of heavy metals resulting in possible risks on human health. For this reason, a pot experiment was conducted between May and August 2016 at the green house of the Department of Forestry and Environmental Management, Michael Okpara University of Agriculture, Umudike. The experiment was specifically aimed at evaluating the influence of kaoline mined soil samples on the growth of three herbaceous plant species (Chromolaena odorata, Ipomoea involucrata and Mariscus alternifolius) commonly found at abandoned kaolin mining site at Ohiya in Umuahia South East, Nigeria. The experiment was laid out in a Randomized Complete Block Design with Six (6) replicates. Data were collected on Plant height, Number of leaves, fresh and dry weight. Data were analyzed using Analysis of Variance (ANOVA). Mean separations were done using Fisher LSD at 0.05% probability. Two mean values were subjected to studentized-t-test using statistical analytical system (SAS) software, version 8.0. The result showed that the kaolin mined soil sample significantly affected all the growth parameters measured during the study. Keywords: Soil, Heavy metals, herbaceous plants ...

Prevalence and Drug Resistance Patterns of Staphylococcus aureus in Food Producing Animals, Their Products and Humans

Review Article of International Journal of Bioscience and Medicine Review on the Prevalence and Drug Resistance Patterns of Staphylococcus aureus in Food Producing Animals, Their Products and Humans Fitsum Tessema* Fitsum Tessema, Hawassa Agricultural Research Center, P.O.Box 2126, Hawassa, Ethiopia Staphylococcus aureus is a gram positive bacterium that belongs to coagulase positive staphylococcus. It is widely distributed in the environment and causes diseases due to direct infection or due to the production of toxins by the bacteria. The prevalence of S. aureus ranges from 4% to 83% in different countries of the world from samples of food producing animals and their products. In Ethiopia the lowest and highest prevalence is reported as 4.2% and 48.75%, respectively. All mammals and birds are susceptible to colonization with S. aureus. Staphylococcus aureus can be treated with a wide range of antibiotics but there are efficient and inefficient antibiotics. Antibiotic resistance tests that have been conducted in different countries revealed that S. aureus were highly resistant to Ampicillin, Cloxacillin and Penicillin and less susceptible to Vancomycin and Rifampicin. The emergence of methicillin resistant Staphylococcus aureus in animal and human has become a worldwide problem. Keywords: Staphylococcus aureus, Prevalence, Drug resistance, Food producing animal ...