Dr. Surabhi Mishra
Dept. of Microbiology
University of Iowa, IA – 52242
Biography & Research Interest
Welcome Dr. Surabhi Mishra to Join the Editorial Board of American Journal of Microbiology and Immunology. Dr. Surabhi Mishra is a Resarch Scientist at Dept. of Microbiology in University of Iowa, IA – 52242
University of Iowa, Iowa City, IA, USA Nov, 2015 – till date
Post Doctoral Research Associate (Advisor: Prof. David Weiss and Prof. Craig Ellermeier) Project title: Investigation of the regulatory elements involved in bistable expression of toxin genes in Clostridium difficile.
University of Iowa, Iowa City, IA, USA Oct, 2014 – Oct, 2015
Post Doctoral Research Associate (Advisor: Prof. Blaise Boles) Project title: Deciphering the mechanism of heparin stimulated biofilm formation by Staphylococcus aureus. Heparin enhances biofilm biomass in S. aureus independent of time of addition in cultures. Heparin mimics eDNA component of biofilm matrix and interacts with a set of eDNA binding proteins that are present on cell surface to enhance biofilm biomass.
University of Illinois Urbana, IL, USA Aug, 2009- Aug, 2012
Post Doctoral Fellow (Advisor: Prof. James Imlay) Project title: Investigation of the basis of obligate anaerobiosis using a model system, Bacteroides thetaiotaomicron. An obligate anaerobe, B. thetaiotaomicron, utilizes distinct set of H2O2 scavenging enzymes for growth under anoxic and oxic conditions, respectively. Rubrerythrin and reverse rubrerythrin act as H2O2 scavenger under anoxic growth, which become inactive upon air – exposure. Catalase and alkylhydroperoxidase (Ahp) function as H2O2 scavengers under aerobic growth conditions. Using H2O2 scavenging deficient mutants of B. thetaiotaomicron and E. coli showed that obligately anaerobic bacteria produce H2O2 at a rate much higher than aerobic bacteria. OxyR regulated stress response is required for survival of air – exposed cells of B. thetaiotaomicron.
Indian Institute of Technology Bombay, India Feb, 2003 – June 2009
Graduate student (Advisor: Prof. Santosh Noronha) Project: Regulation of oxidative stress in Bacillus subtilis and analysis of oxidative stress regulatory gene circuits in bacteria. In a Gram – positive model bacterium, B. subtilis, RecA mediated SOS response and manganese protects the vegetative cells from hypochlorous acid (HOCl) induced oxidative stress. Other oxidative stress defense mechanisms such as PerR and SigB gets induced but fail to provide any protection. A power – law formalism based mathematical model was used to predict the key design features of a stable gene circuit that is involved in the regulation of oxidative stress in bacteria. Stability of the oxidative stress gene circuits is dependent on negative autoregulation. Reversible and irreversible mode of sensing the signal as observed in the case of OxyR and PerR, respectively, affects the efficiency and robustness of the circuit.
Indian Institute of Technology Kharagpur, India July, 2001 – Jan, 2003
M. Tech. (Advisor: Prof. S. Dey and Prof. A. K. Ghosh) Expression of human fibroblast growth factor VIII in tobacco plants The gene encoding for human FGF8 was cloned in a T-DNA binary vector (pCGN series) in Agrobacterium tumefaciens. Integration of FGF8 in tobacco was achieved by: a) particle bombardment of tobacco leaves using plasmid DNA adsorbed on gold particles; b) infecting the tobacco leaves by recombinant A. tumefaciens strain.
University of Calcutta, Kolkata, India Jan, 2000 – Aug, 2000
M. Sc. (Prof. M. B. Choudhary) Project: Structure-function correlation of plant nucleolus. This project involved a comparison of nucleolar number and sizes in plant cells derived from callus and differentiating tissues. The species analyzed were Bacopa monnieri, Nicotiana tabacum, Nigella sativa, and Allium cepa.
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