Research Article of American Journal of Agricultural Research
Impact of application of rock phosphate (RP) inoculated with phosphate solubilizing fungi (PSF) as a fertilizer on total nitrogen (TN), total organic carbon (TOC) and microbial count (MC) in clay soil
Hefnawy M. A., Gharieb M. M., Omima A. Eissa, Asmaa M. A. Ammar*
Botany Dept. Faculty of Science, Menoufia University, Egypt.
In this study the effect of four treatments of phosphate fertilizers (NS, RP, SP & RP inoculated with PSF (A. niger & A. fumigatus) using three doses (RD, 1/2 RD&2RD) on TN, OC and MC of Nile delta clay soil and its impact on some growth parameters of phasoleous vulgaris was monitored. The Results indicated that OC content of clay soil was positively affected by the amendment of soil with inoculated RP. Inoculation of RP with A.niger culture increased the OC content of the soil by 4.91%, 18.28%, 14.38% and 19.61% comparing to A.fumigatus, NS, RP and SP respectively. the highest soil organic carbon content obtained when soil was amended with inoculated RP in 1/2RD it increased by 1.02% and 7.232% comparing to the amendment of with RD and 2RD. RP inoculated with A.niger culture clay soil showed the highest TN content when amended with 2RD the presence of phasloeus vulgaris plant it was 19.19%. Different phosphate treatments affected MC in clay soil to reach the highest count when inoculated rock phosphate with the RD as a fertilizer it increased by5.66% & 20.75% case of A.fumigatus & A.niger respectively comparing to NS. The lowest count obtained when SP was used as a fertilizer, it decreased by 26.4% comparing to NS.
Keywords:phosphate solubilizing fungi, phosphate biofertilizers, inoculated rock phosphate, soil organic carbon content, soil total nitrogen content.
How to cite this article:
Hefnawy M. A., Gharieb M. M., Omima A. Eissa, Asmaa M. A. Ammar. Impact of application of rock phosphate (RP) inoculated with phosphate solubilizing fungi (PSF) as a fertilizer on total nitrogen (TN), total organic carbon (TOC) and microbial count (MC) in clay soil. American Journal of Agricultural Research, 2018,3:20. DOI:10.28933/ajar-2018-06-2801
1. Akram, A., Fatima, M., Ali, S., Jilani, G., & Asghar, R. (2007). Growth, yield and nutrients uptake of sorghum in response to integrated phosphorus and potassium management. Pakistan Journal of Botany, 39(4), 1083.
2. American Public Health, A., Taras, M. J., Water Pollution Control, F., & American Water Works, A. (1975). Standard methods for the examination of water and wastewater. Prepared and published jointly by American Public Health Association, American Water Works Association [and] Water Pollution Control Federation. Joint editorial board: Michael J. Taras [et al.]. New York.
3. Assem, S. K., Hussein, E. H., Hussein, H. A., & Basry, M. (2009). Genetic transformation of the Nicotiana protein kinase (NPK1) gene confers osmotic tolerance in Egyptian maize. Australian journal of basic and applied sciences, 3(2), 828-835.
4. Aziz, T., Rahmatullah, M. A., Maqsood, M. A., Tahir, I. A., & MA, C. (2006). Phosphorus utilization by six Brassica cultivars (Brassica juncea L.) from tri-calcium phosphate; a relatively insoluble P compound. Pak. J. Bot, 38(5), 1529-1538.
5. Bacha, C. J., & Vinicios de Carvalho, L. (2014). What Explains the Intensification and Diversification of Brazil’s Agricultural Production and Exports from 1990 to 2012?
6. Bartlett, G., Craze, B, Stone, MJ & Crouch, R (1994). Guidelines for Analytical Laboratory Safety. Department of Conservation & Land Management, Sydney.
7. Black, C. A., Evans, D. D., Ensminger, L. E., White, J. L., Clark, F. E., & Dinauer, R. C. (1965). Methods of soil analysis. Part 2. Chemical and microbiological properties. Madison, Wis.: American Society of Agronomy.
8. Deressa, T., Hassan, R. M., Alemu, T., Yesuf, M., & Ringler, C. (2008). Analyzing the determinants of farmers’ choice of adaptation methods and perceptions of climate change in the Nile Basin of Ethiopia: Intl Food Policy Res Inst.
9. Ditta, A., Muhammad, J., Imtiaz, M., Mehmood, S., Qian, Z., & Tu, S. (2018). Application of rock phosphate enriched composts increases nodulation, growth and yield of chickpea. International Journal of Recycling of Organic Waste in Agriculture, 7(1), 33-40. doi:10.1007/s40093-017-0187-1
10. Elias, F., Muleta, D., & Woyessa, D. (2016). Effects of Phosphate Solubilizing Fungi on Growth and Yield of Haricot Bean (Phaseolus vulgaris L.) Plants (Vol. 8).
11. Fageria, N., Baligar, V., Moreira, A., & Portes, T. (2010). Dry bean genotypes evaluation for growth, yield components and phosphorus use efficiency. Journal of Plant Nutrition, 33(14), 2167-2181.
12. Greene, J., Bartels, C., Warren-Hicks, W., Parkhurst, B., & Linder, G. (1988). Protocols for short-term toxicity screening of hazardous-waste sites. Retrieved from
13. Gunes, A., Inal, A., Alpaslan, M., & Cakmak, I. (2006). Genotypic variation in phosphorus efficiency between wheat cultivars grown under greenhouse and field conditions. Soil Science and Plant Nutrition, 52(4), 470-478.
14. Hefnawy, M., Gharieb, M., Omima, A. E., & Asmaa, M. (2017). Enhancing of rock phosphate solubility and plant availability in clay soil through inoculation of the rock with phosphate solubilizing fungal cultures and the effect of the amendment of soil with inoculated rock phosphate on phaseolus vulgaris plant growth. Journal of Innovations in Pharmaceutical and Biological Sciences (JIPBS), Vol 4 (1),, 17-24,.
15. Jenkins, D., & Medsker, L. L. (1964). Brucine Method for the Determination of Nitrate in Ocean, Estuarine, and Fresh Waters. Analytical Chemistry, 36(3), 610-612. doi:10.1021/ac60209a016
16. Lützow, M., Kögel-Knabner, I., Ekschmitt, K., Flessa, H., Guggenberger, G., Matzner, E., & Marschner, B. (2007). SOM fractionation methods: Relevance to functional pools and to stabilization mechanisms (Vol. 39).
17. Martin, J. P. (1950). Use of acid rose bengal and streptomycin in the plate method for estimating soil fungi. . Soil Sci., 69, 215–233.
18. McLeod, S. (1973). Studies on wet oxidation procedures for the determination of organic carbon in soils. CSIRO Division of Soils, Notes on Soil Techniques, 73-79.
19. Mickan, B. S., Abbott, L. K., Fan, J., Hart, M. M., Siddique, K. H. M., Solaiman, Z. M., & Jenkins, S. N. (2018). Application of compost and clay under water-stressed conditions influences functional diversity of rhizosphere bacteria. Biology and Fertility of Soils, 54(1), 55-70. doi:10.1007/s00374-017-1238-5
20. Moharana, P. C., Meena, M. D., & Biswas, D. R. (2018). Role of Phosphate-Solubilizing Microbes in the Enhancement of Fertilizer Value of Rock Phosphate Through Composting Technology.
21. Njira, K. O., & Nabwami, J. (2015). A review of effects of nutrient elements on crop quality. African Journal of Food, Agriculture, Nutrition and Development, 15(1), 9777-9793.
22. Prokopy, W. (1992). Determination of Total Phosphorus by Flow Injection Analysis Colorimetry (Acid Persulfate Digestion Method): Quickchem Method 10-115-01-1-F. Lachat Instruments, Loveland, CO, USA.
23. Raliya, R., Tarafdar, J. C., & Biswas, P. (2016). Enhancing the Mobilization of Native Phosphorus in the Mung Bean Rhizosphere Using ZnO Nanoparticles Synthesized by Soil Fungi. J. Agric. Food Chem. Journal of Agricultural and Food Chemistry, 64(16), 3111-3118.
24. Shabnam, R., & Iqbal, M. T. (2016). Understanding phosphorus dynamics on wheat plant under split-root system in alkaline soil. Braz J Sci Technol Brazilian Journal of Science and Technology, 3(1), 1-16.
25. Stevenson, F. J. (1994). Humus chemistry: genesis, composition, reactions. New York: John Wiley and Sons.
26. Testing, A. S. f., & Materials. (1976). Annual book of ASTM standards: American Society for Testing and Materials.
27. Wahid, F., Sharif, M., Steinkellner, S., Khan, M. A., Marwat, K. B., & Khan, S. A. (2016). Inoculation of arbuscular mycorrhizal fungi and phosphate solubilizing bacteria in the presence of rock phosphate improves phosphorus uptake and growth of maize. Pakistan journal of botany., 48(2), 739-748.
28. Youssef, S. M., Riad, G. S., & Abd Elhady, S. A. (2017). Effect of Phosphorus Sources and Arbuscular Mycorrhizal Inoculation on Growth and Productivity of Snap Bean (Phaseolus vulgaris L.). Gesunde Pflanzen, 69(3), 139-148. doi:10.1007/s10343-017-0398-4