Mineralization of phosphorous by phosphate solubilising bacteria isolated from a vertisol


Mineralization of phosphorous by phosphate solubilising bacteria isolated from a vertisol

Vivek Singh, Bharati Kollah, Santosh Ranjan Mohanty*

Indian Institute of Soil Science, Nabibagh, Bhopal, India 462038


The current experiment unravels P solubilisation potential of soil under long term fertilizer application. Soil samples collected from a 20 years old long term experimental field. Treatments included fallow (no fertilizer, no crop), control (no fertilizer, with crop), 100% N, 100% NP, 100% NPK, and 100% NPK+FYM. P solubilisation potential of soils determined using Ca3(PO4)2 as inorganic insoluble P source. Abundance of total bacteria, phosphate solubilising bacteria (PSB) estimated along with the efficient PSB isolated to evaluate P solubilisation potential using Ca3(PO4)2, rock phosphate and sodium phytate as P sources. P solubilisation rate was highest in 100% NP and lowest in fallow. Abundance of total eubacteria and phosphate solubilizing bacteria (PSB) was high in 100% NP and low in fallow. The 16S rRNA sequences of the isolates were homologues to Paraburkholderia sp. The efficient PSB isolate solubilised Ca3(PO4)2, rock phosphate as well as sodium phytate. Acid phosphatise activity was highest in Ca3(PO4)2 and lowest in sodium phytate. Study concludes that P solubilisation in vertisol under long term fertilizer application is regulated by nutrients, particularly P fractions and abundance of PSB. The PSB solubilise different P sources by reducing pH of medium as well as through acid phosphatase attributes.


Keywords: Phosphorus solubilisation; long term fertilizer; vertisol; bacteria; 16S rRNA

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How to cite this article:
Vivek Singh, Bharati Kollah, Santosh Ranjan Mohanty. Mineralization of phosphorous by phosphate solubilising bacteria isolated from a vertisol. American Journal of Agricultural Research, 2020,5:77.


References:

1. Alori ET, Glick BR, Babalola OO (2017) Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Front Microbiol 8:971
2. Beck MA, Sanchez PA (1994) Soil phosphorus fraction dynamics during 18 years of cultivation on a Typic Paleudult. Soil Sci Soc Am J 58:1424–1431
3. Cosgrove DJ (1963) The chemical nature of soil organic phosphorus. I. Inositol phosphates. Soil Res 1:203–214
4. Drijber RA, Doran JW, Parkhurst AM, Lyon DJ (2000) Changes in soil microbial community structure with tillage under long-term wheat-fallow management. Soil Biol Biochem 32:1419–1430
5. Duncan EW, King KW, Williams MR, et al (2017) Linking soil phosphorus to dissolved phosphorus losses in the Midwest. Agric Environ Lett 2:
6. Eo J, Park K-C (2016) Long-term effects of imbalanced fertilization on the composition and diversity of soil bacterial community. Agric Ecosyst Environ 231:176–182
7. Feng G, Song YC, Li XL, Christie P (2003) Contribution of arbuscular mycorrhizal fungi to utilization of organic sources of phosphorus by red clover in a calcareous soil. Appl Soil Ecol 22:139–148
8. Foltran EC, Rocha JHT, Bazani JH, et al (2019) Phosphorus pool responses under different P inorganic fertilizers for a eucalyptus plantation in a loamy Oxisol. For Ecol Manag 435:170–179
9. Goldstein AH (1995) Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by gram negative bacteria. Biol Agric Hortic 12:185–193
10. Hermans C, Hammond JP, White PJ, Verbruggen N (2006) How do plants respond to nutrient shortage by biomass allocation? Trends Plant Sci 11:610–617
11. Hofmann K, Heuck C, Spohn M (2016) Phosphorus resorption by young beech trees and soil phosphatase activity as dependent on phosphorus availability. Oecologia 181:369–379
12. Horii S, Matsuno T, Tagomori J, et al (2013) Isolation and identification of phytate-degrading bacteria and their contribution to phytate mineralization in soil. J Gen Appl Microbiol 59:353–360
13. Hsu P-CL, O’Callaghan M, Condron L, Hurst MR (2018) Use of a gnotobiotic plant assay for assessing root colonization and mineral phosphate solubilization by Paraburkholderia bryophila Ha185 in association with perennial ryegrass (Lolium perenne L.). Plant Soil 425:43–55
14. Jackson ML (1958) Soil Chemical Analysis. Prentice- Hall, Inc, Englewood, Cliffs, New Jersey
15. Li L, Tang C, Rengel Z, Zhang F (2003) Chickpea facilitates phosphorus uptake by intercropped wheat from an organic phosphorus source. Plant Soil 248:297–303
16. Li W, Angel R, Kim S-W, et al (2016) Impacts of dietary calcium, phytate, and nonphytate phosphorus concentrations in the presence or absence of phytase on inositol hexakisphosphate (IP6) degradation in different segments of broilers digestive tract. Poult Sci 95:581–589
17. Lin T-F, Huang H-I, Shen F-T, Young C-C (2006) The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74. Bioresour Technol 97:957–960
18. Liu Z, Li YC, Zhang S, et al (2015) Characterization of phosphate-solubilizing bacteria isolated from calcareous soils. Appl Soil Ecol 96:217–224
19. Menezes-Blackburn D, Giles C, Darch T, et al (2018) Opportunities for mobilizing recalcitrant phosphorus from agricultural soils: a review. Plant Soil 427:5–16
20. Miao Y, Stewart BA, Zhang F (2011) Long-term experiments for sustainable nutrient management in China. A review. Agron Sustain Dev 31:397–414
21. Nannipieri P, Giagnoni L, Landi L, Renella G (2011) Role of phosphatase enzymes in soil. In: Phosphorus in action. Springer, pp 215–243
22. Roberts TL, Johnston AE (2015) Phosphorus use efficiency and management in agriculture. Resour Conserv Recycl 105:275–281
23. Rodriguez H, Fraga R (1999) Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv 17:319–339
24. Schlesinger WH, Bruijnzeel LA, Bush MB, et al (1998) The biogeochemistry of phosphorus after the first century of soil development on Rakata Island, Krakatau, Indonesia. Biogeochemistry 40:37–55
25. Sharon JA, Hathwaik LT, Glenn GM, et al (2016) Isolation of efficient phosphate solubilizing bacteria capable of enhancing tomato plant growth. J Soil Sci Plant Nutr 16:525–536
26. Shen J, Li R, Zhang F, et al (2004) Crop yields, soil fertility and phosphorus fractions in response to long-term fertilization under the rice monoculture system on a calcareous soil. Field Crops Res 86:225–238
27. Song O-R, Lee S-J, Lee Y-S, et al (2008) Solubilization of insoluble inorganic phosphate by Burkholderia cepacia DA23 isolated from cultivated soil. Braz J Microbiol 39:151–156
28. Stephen J, Shabanamol S, Rishad KS, Jisha MS (2015) Growth enhancement of rice (Oryza sativa) by phosphate solubilizing Gluconacetobacter sp.(MTCC 8368) and Burkholderia sp.(MTCC 8369) under greenhouse conditions. 3 Biotech 5:831–837
29. Tallapragada P, Seshachala U (2012) Phosphate-solubilizing microbes and their occurrence in the rhizospheres of Piper betel in Karnataka, India. Turk J Biol 36:25–35
30. Van Vuuren DP, Bouwman AF, Beusen AH (2010) Phosphorus demand for the 1970–2100 period: a scenario analysis of resource depletion. Glob Environ Change 20:428–439
31. Wang Q, Wang W, He X, et al (2017) Changes in soil properties, X-ray-mineral diffractions and infrared-functional groups in bulk soil and fractions following afforestation of farmland, Northeast China. Sci Rep 7:12829
32. Yoon SJ, Choi YJ, Min HK, et al (1996) Isolation and identification of phytase-producing bacterium, Enterobacter sp. 4, and enzymatic properties of phytase enzyme. Enzyme Microb Technol 18:449–454
33. Zhang ZY, Huang L, Liu F, et al (2016) Characteristics of clay minerals in soil particles of two Alfisols in China. Appl Clay Sci 120:51–60
34. Zhong W, Gu T, Wang W, et al (2010) The effects of mineral fertilizer and organic manure on soil microbial community and diversity. Plant Soil 326:511–522
35. Zhou Z, Hu D, Ren W, et al (2015) Effect of humic substances on phosphorus removal by struvite precipitation. Chemosphere 141:94–99