Research Article of American Journal of Agricultural Research
Induce systemic Resistance against root rot and wilt diseases in faba bean as a possible and effective control
Manal, Sayed Mohammed Khalila*; Mohamed Hassan Abdel-Rahem Hassanb; Amer Fayz Mahmoudb and Kadry Mostafa Mohamed Morsya
aPlant Pathology Dept., Faculty of Agric., Assiut Univ., 71526 Assiut, Egypt
bLeguminous Crops and Fodder Dis. Res. Dep., Plant Pathol. Res. Instit., ARC, Giza 12619, Egypt
Root rot and wilt diseases caused by soil borne pathogenic fungi is the most sever disease attacks faba bean plants in New valley Governorate, Egypt. Efficacies of some plant resistance elicitors viz.: salicylic Acid (SA), ascorbic acid (AA), humic acid (HA) and Bion was evaluated as faba bean seed soaking, compared to untreated control treatment under greenhouse and field conditions. Under laboratory conditions, all the tested chemical inducers have no effect or little effect on linear growth of all tested pathogenic fungal isolates. On the other hand, all tested chemical inducers at different concentrations were decreased the root rot and wilt severity. Salicylic acid at 400 and Bion at 1000 ppm recorded the lowest root rot and wilt severity. All chemical inducers individually or in combination with R. leguminosarum significantly decreased root rot and wilt diseases under greenhouse and field conditions as well as increased total yield /feddan under field conditions. The combination between chemical inducers and R. leguminosarum more effective for controlling root rot and wilt diseases and increased seed yield/feddan than individually treatment. Application of SA and Bion + R. leguminosarum recorded the lowest percentage of root rot and wilt severity and the highest plant growth and yield parameter during both growing seasons.
Keywords: Faba bean, Root rot and wilt diseases, Chemical inducers, Seed yield
How to cite this article:
Manal, Sayed Mohammed Khalil; Mohamed Hassan Abdel-Rahem Hassan; Amer Fayz Mahmoud and Kadry Mostafa Mohamed Morsy. Induce systemic Resistance against root rot and wilt diseases in faba bean as a possible and effective control. American Journal of Agricultural Research, 2020,5:92.
1. Abdel-Kader MM, El-Mougy NS, Lashin SM (2011). Essential oils and Trichoderma harzianum as an integrated control measure against faba bean root rot pathogens. J. Plant Prot. Res., 51: 306–313.
2. Abdel-Monaim MF (2013). Improves biocontrol of damping-off and root rot/wilt of faba bean by salicylic acid and hydrogenperoxide. Mycobiology, 41(1): 47-55.
3. Abdel-Monaim MF (2017). Evaluation of the accumulation of pathogenesis related (PR) proteins and phenolic compounds in response to biotic and abiotic elicitors as mechanism for immune response to Fusarium wilt disease in faba bean. J. of Plant Pathol. & Microbiology, 8(2): 1-8.
4. Abdel-Monaim MF and Ismail ME (2010). The use of antioxidants to control root rot and wilt diseases of pepper. Not Sci Biol, 2 (2): 46-55.
5. Abdel-Monaim MF, Ismail ME and Morsy KM (2011). Induction of systemic resistance of benzothiadiazole and humic acid in soybean plants against Fusarium wilt disease. Mycobiol. 39: 290-298.
6. Anderson AJ (1988). Elicitors, the hypersensitive response and phytoalexins. Pages 103-1 10 in: Physiology and Biochemistry of Plant Microbial Interactions. N. T. Keen, T. Kosuge and L. L. Walling, eds. Am. Soc. Plant Physiol. Rockville, MD.
7. Bush DS (1993). Regulation of cytosolic calcium in plants. Plant Physiol., 103:7–13.
8. Chavan JK, Kute LS, Kadam SS (1989). Soybean. In: Salunkhe DD, Kadam SS, editors. CRC handbook of world food legumes. Boca Raton: CRC Press; pp. 223–245.
9. Chen Y, M. De Nobili and T. Aviad. 2004. Stimulatory effects of humic substances on plant growth. In: Magdoff F, Weil RR, editors. Soil organic matter in sustainable agriculture. Boca Raton: CRC Press: 103-130.
10. El-Sayed (Sahar) A, Mousa (Abeer) M (2015). Effect of some algal filtrates and chemical inducers on root-rot incidence of faba bean. Agri. Res. and Tech., 1(1): 1-5.
11. Gomez KA and AA (1984). Gomez Statistical procedures for agricultural research. New York: Wiley Interscience Publication; p. 678
12. Hassan N, Elsharkawy MM, Shivanna MB, Meera MS, Hyakumachi M (2014). Elevated expression of hydrolases, oxidase and lyase in susceptible and resistant cucumber cultivars systemically induced with plant growth-promoting fungi against anthracnose. ACTA AGR SCAND B- SP 64: 155–164.
13. Jarvis WR (1988). Fusarium crown root rot of tomatoes. Phytoprotection. ;69:49–64.
14. Khalil (Manal) SM (2019). Utilizing Some Disease Resistance Inducing Agents and Magnetized Water in the Management of Root Rot and Wilt of Faba Bean. Ph. D. Thesis, Fac. Agric., Assuit University.
15. Lawton K, Friedrich L, Hunt M, Weymann K, Delaney T, Kessmann H, Staub T and Ryals J (1996). Benzothiadiazole induces disease resistance in Arabidopsis by activation of the systemic acquired resistance signal transduction pathway. Plant J. 10:71–82.
16. Martinez C, Baccou JC, Bresson E, Bessac Y, Daniel JF, Jalloul A, Montillet JL, Geiger JP, Assigbetse K, Nicole M (2000). Salicylic acid mediated by the oxidative burst is a key molecule in local and systemic response of cotton challenged by an avirulent race of Xanthomonas campestris pv. malvacearum. Plant Physiol., 122: 757–766.
17. Mazen MM (2004). Resistance Induction Against Diseases of Faba Bean Crop. Ph.D. Thesis, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.
18. Meena B, Marimuthu T, Velazhahan R (2001). Salicylic acid induced resistance in Groundnut against late leaf spot caused by Cercosporidium personatum. J. Mycol. Pl. Pathol. 31, 139–145.
19. Pirasteh Anosheh H, Emam Y, Ashraf M and Foolad MR (2012). Exogenous application of salicylic acid and chlormequat chloride alleviates negative effects of drought stress in wheat. Advanced Studies in Biology, 4 (11): 501 – 520.
20. Sarwar N, Ch MH, Haq I, Jamil FF (2005). Induction of systemic resistance in chickpea against Fusarium wilts by seed treatment with salicylic acid and Bion. Pak. J. Bot.; 37:989–995.
21. Toppan A, Roby D and Esquerre-Tugaye MT (1982). Cell surfaces in plant-microorganism interactions: iii. in vivo effect of ethylene on hydroxyproline-rich glycoprotein accumulation in the cell wall of diseased plants. Plant Physiol. 70(1):82–86.
22. Ward ER, Uknes SJ, Williams SC, Dincher SS, Wiederhold DL, Alexander DC, Ahl-Goy P, Métraux JP, Ryals JA (1991). Coordinate gene activity in response to agents that induce systemic acquired resistance. Plant Cell, 3: 1085-1094.
23. Ye XS, Jarlfors U, Tuzun S, Pan SQ and Kuc J (1992). Biochemical changes in cell walls and cellular responses of tobacco leaves related to systemic resistance to blue mold (Peronospora tabacina) induced by tobacco mosaic virus. Canadian J. of Botany, 70: 49–57