Comparative analyses of plantain vivoplants responses to different clam shells and Tithonia diversifolia treatments in terms of growth promotion and induced resistance against Mycosphaerella fijiensis

Comparative analyses of plantain vivoplants responses to different clam shells and Tithonia diversifolia treatments in terms of growth promotion and induced resistance against Mycosphaerella fijiensis

C.A. Ewané1,2* and T. Boudjeko1,2

1Laboratory of Phytoprotection and Plant Valorization, Biotechnology Centre, University of Yaoundé 1, Messa-Yaoundé, Cameroon. 2Department of Biochemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon.

The seeds availability and quality are the main constraints for agricultural explosion of plantain productivity in sub-Saharan Africa countries. Plantain vivoplants were generated and submitted to different treatments in the nursery, the plant responses were analysed and compared in other to determine the best treatment influencing the growth promotion and induced resistance to Mycosphaerella fijiensis. Plantain explants and vivoplants were treated with five different treatments: clam shells powder (T1), clam shells and Tithonia diversifolia powder (T2), Tithonia diversifolia flakes (T3), Tithonia diversifolia mulch (T4), Tithonia diversifolia liquid extract (T5). The treatments were applied by their incorporation in the substrate (T1, T2, T3 and T4) or by watering of the whole plant (T5). The germination rate was evaluated and recorded in the greenhouse at the germination and pre-emergence stage, followed by the agromorphological measurements on the vivoplants and their inoculation with Mycosphaerella fijiensis in the shade at the vegetative growth stage. Biochemical analysis was done on the vivoplants leaves tissues. The vivoplants respond positively to all the treatments by a quick germination and emergence, coupled with an important biomarker’s accumulation (total proteins and phenolics). It turns out that the best treatment was T5 (T. diversifolia liquid extract), followed by T4 (T. diversifolia mulch). However, depending on the expected response in the vivoplants, all these treatments have proven to be impactful. Therefore, a combination of Tithonia diversifolia liquid extract (T5) with clams’ shells (T1) could be useful to boost the production at low cost and without chemical inputs of large amount of improved vigorous (clean and less susceptible) planting material, impacting thus the food security and poverty alleviation.

Keywords: plantain (Musa spp.); vivoplants; Tithonia diversifolia; clam shells; Mycosphaerella fijiensis; growth promotion; biofungicide.

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C.A. Ewané and T. Boudjeko. Comparative analyses of plantain vivoplants responses to different clam shells and Tithonia diversifolia treatments in terms of growth promotion and induced resistance against Mycosphaerella fijiensis. American Journal of Agricultural Research, 2020,5:99. DOI: 10.28933/ajar-2020-06-1405


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