ABSENCE OF OXIDATIVE STRESS AND SIRTUINS RECRUITMENT ON CARDIAC TISSUE POST STRESS


ABSENCE OF OXIDATIVE STRESS AND SIRTUINS RECRUITMENT ON CARDIAC TISSUE POST STRESS


Ana Elisa T.S. de Carvalho*, Marco A. Cordeiro, Luana S. Rodrigues, Daniela Ortolani, Regina C. Spadari*

Department of Biosciences, Federal University of São Paulo (UNIFESP), Santos, SP, Brazil


Stress has emerged as a factor associated with cardiovascular disease. Catecholamines released during the stress reaction by the sympathetic nerves and the adrenal medulla couple to β1-and β2-adrenoceptors in the cardiomyocytes membrane enhancing heart function in order to attend the organism demand. This might produce excessive reactive oxygen species what may culminate with oxidative stress and progression of several cardiac diseases. Sirtuins have been described as cardioprotective factors and important regulators of the cellular stress response in the heart. The aim of this work is to investigate the putative participation of oxidative stress and sirtuins in the heart of rats submitted to foot shock stress, an experimental model where there is up regulation of β2-adrenoceptors and downregulation of β1-adrenoceptors. The data have shown that in the myocardium of rats submitted to foot shock stress the H2O2 concentration, catalase and superoxide dismutase activity, NAD+/NADH ratio, as well as the protein expression of sirtuins 1 and 3 were not altered. Pharmacological blockade of the β2-adrenoceptors by ICI118,551, did not modify this scenario. It is concluded that foot shock stress does not cause disruptions in oxidative stress or redox state processes in the myocardium, and consequently, sirtuins are not recruited to stress response.


Keywords: oxidative stress, sirtuins, foot shock stress, β2-adrenoceptor

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How to cite this article:
Ana Elisa T.S. de Carvalho, Marco A. Cordeiro, Luana S. Rodrigues, Daniela Ortolani, Regina C. Spadari. Absence of oxidative stress and sirtuins recruitment on cardiac tissue post stress.American Journal of Cardiology Research and Reviews, 2022, 5:19. DOI:10.28933/ajcrar-2021-10-2205


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