Research Article of American Journal of Microbiology and Immunology
Prevalence and antibiotic resistance of Campylobacter coli and Campylobacter jejuni in Greek swine farms
Dimitrios Papadopoulos1, Evanthia Petridou1, (†) Georgios Filioussis1, Theofilos Papadopoulos1, Konstantinos Papageorgiou1, Maria Chatzistilianou2, Spyridon K. Kritas1
1Department of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece; 2Clinic of Pediatrics-Immunology and Infectious Disease, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece
Campylobacter species are one of four key global causes of human diarrheal diseases, according to W.H.O. It is considered to be the most common bacterial cause of human gastroenteritis in the world. The objective of this study was to estimate the prevalence of Campylobacter coli (C. coli) and Campylobacter jejuni (C. jejuni) in Greek commercial swine farms, and describe the antimicrobial resistance of the isolated strains. A total of 1,000 rectal swabs (50 per farm) were collected from twenty swine farms in Greece. Ten rectal samples had been randomly collected from each of five age-groups (suckling piglets, nursery pigs, grower pigs, finisher pigs, sows). Isolation of Campylobacter spp. was performed using the ISO 10272-1:2017. A PCR method, based on the amplification of mapAC.jejuni and ceuEC.coli specific genes, was used for identification of the isolated strains. All isolates were tested for their susceptibility against gentamycin, erythromycin, ciprofloxacin, tetracycline and meropenem; EUCAST guidelines were used for the interpretation. The results showed that 16 out of the 20 farms (80%) and 491 (49%) of the samples were positive for Campylobacter spp. Prevalence of C.coli was 38% (95% CI 35.1-41.1) and of C.jejuni 10.9% (95% CI 9,1-13.0). Sows were 1.4 times more likely to be colonized by Campylobacter spp than sucking piglets (p<0.05) while nursery and grower pigs were 2.14 and 2 times more likely to be colonized than sows p<0.001). However, colonization was not associated with farm size. High rates of resistance were recorded for tetracycline (67,3%), while 18,1%, 7,3% and 3.9% of the isolates were resistant in ciprofloxacin, erythromycin and gentamycin respectively. Thirty-two of the isolates (6,52%) were classified as multidrug resistant; resistance to meropenem was not found. Our findings indicate high prevalence of C.coli and C.jejuni in Greek pig farms with high resistant rates to tetracycline and ciprofloxacin; this constitutes a potential reservoir for resistance genes spread to the community.
Keywords: Campylobacter, Antimicrobial Resistance, Minimum Inhibitory Concentration (MIC), Antimicrobial Susceptibility Testing (AST), pigs, zoonosis
How to cite this article:
Dimitrios Papadopoulos, Evanthia Petridou,Georgios Filioussis, Theofilos Papadopoulos, Konstantinos Papageorgiou, Maria Chatzistilianou, Spyridon K. Kritas.Prevalence and antibiotic resistance of Campylobacter coli and Campylobacter jejuni in Greek swine farms. American Journal of Microbiology and Immunology, 2020 5:6. DOI: 10.28933/ajmi-2020-02-2605
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